ASSESSMENT OF THE RELATIONSHIP BETWEEN -1562C>T ALLELE OF MMP9 GENE PROMOTER WITH DIABETIC FOOT ULCER IN TYPE 2 DIABETIC PATIENTS REFERRED TO THE CLINIC OF DIABETES & METABOLIC DISEASES

Shafiee, Sahar; Raz, Abbasali; Adibi, Neda; Mansouri, Masoomeh; Annabestani, Zohreh; Mirzaeezadeh, Zahra; Hashemi, Mehrdad; Omidfar, Kobra

Volume 17, Issue 1 (3-2018) ijdld 2018, 17(1): 49-56 | Back to browse issues page

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Shafiee S, Raz A, Adibi N, Mansouri M, Annabestani Z, Mirzaeezadeh Z, et al . ASSESSMENT OF THE RELATIONSHIP BETWEEN -1562C>T ALLELE OF MMP9 GENE PROMOTER WITH DIABETIC FOOT ULCER IN TYPE 2 DIABETIC PATIENTS REFERRED TO THE CLINIC OF DIABETES & METABOLIC DISEASES. ijdld 2018; 17 (1) :49-56
URL: http://ijdld.tums.ac.ir/article-1-5582-en.html

ASSESSMENT OF THE RELATIONSHIP BETWEEN -1562C>T ALLELE OF MMP9 GENE PROMOTER WITH DIABETIC FOOT ULCER IN TYPE 2 DIABETIC PATIENTS REFERRED TO THE CLINIC OF DIABETES & METABOLIC DISEASES

Sahar Shafiee ^*

¹, Abbasali Raz²

, Neda Adibi²

, Masoomeh Mansouri³

, Zohreh Annabestani⁴

, Zahra Mirzaeezadeh⁵

, Mehrdad Hashemi¹

, Kobra Omidfar⁵

1- slamic Azad University, Tehran Medical Branch, Iran
2- Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran
3- 3. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
4- Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
5- Biosensor Research Center, Endocrinology and Metabolism Molecular–Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Abstract: (9900 Views)

Background: Type 2 diabetes is the most common type of diabetes, and a complex endocrine metabolic disorder that one of its main complications is diabetic foot ulcers. Matrix metalloproteinases (MMPs) are one of the key enzymes in the reconstruction of extracellular matrix which have proteolytic activity. The aim of this research is based on evaluating relationship between -1562 C>T allele at MMP-9 gene promoter with diabetic foot ulcer in type II diabetic patients. If such correlation proves, it can be used as a prognostic biomarker in patients with high-risk.
Methods: This is a case-control study. The single nucleotide polymorphism of -1562C>T allele of MMP9 gene promoter was genotyped by hit Tetra ARMS PCR technique in 100 diabetic patients with foot ulcer grade 1 or 2 as the case group according Wagner classification and in 100 diabetic patients without foot ulcer as the control group. Results: The Chi-square test revealed significant difference in genotype frequency of CC, CT and TT alleles of -1562C>T allele of MMP9 gene promoter between case and control groups (P=0.000).
Conclusion: According to this study, there is a relationship between -1562C>T allele of MMP9 gene promoter with diabetic foot ulcer in type2 diabetes patients. Thus we can introduce this biomarker for evaluation of risk and prognosis of diabetic foot ulcers.