Volume 15, Number 3 (3-2017)                   ijdld 2017, 15(3): 183-191 | Back to browse issues page


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Teimouri N, Nayeri H. SERUM CYTOKERATIN-18 FRAGMENT LEVELS, PARAOXONASE ACTIVITY AND LIPID PROFILE OF NON-ALCOHOLIC FATTY LIVER IN IRAN. ijdld. 2017; 15 (3) :183-191
URL: http://ijdld.tums.ac.ir/article-1-5382-en.html

1- Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2- Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran , nahidteimouri1400@gmail.com
Abstract:   (2462 Views)

Background: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease is increasing in adults and children worldwide. Obesity, insulin resistance or diabetes type II, hyperlipidemia and hypertriglyceridemia plays a major role in the epidemiology of this disease. Cytokeratin 18 (CK-18) the major intermediate filament protein in the liver is a marker of increased hepatocyte apoptosis. The aim of this study was to determinate CK-18 level as a marker of hepatocyte apoptosis and paraoxonase as a biochemical marker for lipid peroxidation.

Methods: This case–control study was done on 51 subjects with confirmed NAFLD by ultrasound and 30 healthy individuals. CK-18 is proposed as a biomarker alternative cell death. The serum was used for measurement of the apoptosis-associated neo-epitope in the C-terminal domain of CK-18 by the M30-Apoptosense ELISA kit. The M30 detection antibody recognizes a neo-epitope mapped to positions 387 to 396 of CK18, so called CK18-Asp396 that is only revealed after caspase cleavage of the protein and is postulated as a selective biomarker of apoptosis. Serum PON1 activity was assayed using a synthetic substrate. Paraoxon substrate (diethyl-p nitrophenylphosphate), was deliberated using the increase of absorbance at 412 nm at 37 ◦C. 

Results: There were significant differences regarding serum cytokeratin 18 (p=0.005), paraoxonase activity (p=0.03), triglycerides (p=0.04) and low-density lipoprotein (p=0.04) between NAFLD and healthy subjects. Between CK-18 and paraoxonase with the early stages of fatty liver disease are associated.

Conclusion: This study suggests that serum levels of cytokeratin 18 can be useful in predicting non-alcoholic fatty liver disease. Paraoxonase activity (PON1) should be considered a biochemical marker of lipid peroxidation and the need for follow-up in patients with NAFLD

Keywords: Non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), Cytokeratin 18 (CK-18), Paraoxonases

Full-Text [PDF 363 kb]   (970 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/07/20 | Accepted: 2016/01/31 | Published: 2016/04/20

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