Volume 16, Number 1 (8-2017)                   ijdld 2017, 16(1): 9-16 | Back to browse issues page


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Hassanzade S, Raz A, Mansouri M, Mirzaeezadeh Z, Larijani B, hashemi M et al . ASSESSMENT OF THE RELATIONSHIP BETWEEN (THR399ILE) POLYMORPHISM OF TLR4 GENE WITH DIABETIC FOOT ULCER IN TYPE 2 DIABETIC PATIENTS REFERRED TO THE CLINIC OF DIABETES & METABOLIC DISEASES. ijdld. 2017; 16 (1) :9-16
URL: http://ijdld.tums.ac.ir/article-1-5494-en.html

1- Islamic Azad University, Tehran Medical Branch, Iran
2- Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran
3- Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
4- Biosensor Research Center, Endocrinology and Metabolism Molecular–Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
5- Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
6- Biosensor Research Center, Endocrinology and Metabolism Molecular–Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran , omidfar@tums.ac.ir
Abstract:   (533 Views)
Background: Type 2 diabetes is a metabolic disorder characterized by high blood sugar levels that can damage nerves. Many organs are affected ،especially the foot that leading to loss of sensation. These factors make favorable conditions for the development of diabetic foot ulcers. Polymorphisms (Thr399Ile) of Toll Like Receptor4 (TLR4) gene due to malfunction of TLR4 protein which plays an important role in immunity. The purpose of this study was to determine the parameters which are affecting the imbalance resulting in chronic inflammation and wound healing. By showing the relationship between single nucleotide polymorphism (Thr399Ile) of TLR4 gene with diabetic foot ulcer we can identify a biomarker for prediction of diabetic foot ulcer.
Methods: This is a case-control study. Single nucleotide polymorphisms of TLR4 gene were genotyped by hit Tetra ARMS PCR technique. In this study, 100 and 120 diabetic patients with and without foot ulcer were selected as the cases and controls, respectively.
Results: The Chi-square test revealed significant difference in frequency of TT, CC and TC alleles of (Thr399Ile) between case and control groups (P=0.021).
Conclusion: According to this study, there is a relationship between single nucleotide polymorphisms (Thr399Ile) of TLR4 gene with diabetic foot ulcer in type 2 diabetes patients
Full-Text [PDF 1588 kb]   (74 Downloads)    
Type of Study: Research | Subject: Special
Received: 2016/04/3 | Accepted: 2016/07/16 | Published: 2017/08/28

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