Volume 16, Issue 6 (10-2017)
ijdld 2017, 16(6): 331-338 |
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Sadeghian far Z, Sheikhi A, Gheibi hayat S M. The Effect Of Garlic Extract’s On Expression Of TNF-α And IFNγ By Peripheral Blood Mononuclear Cells Of Patients With Diabetes Mellitus Type Ii Admitted To Ganjavian Dezful Hospital In 2011-2013. ijdld 2017; 16 (6) :331-338
URL: http://ijdld.tums.ac.ir/article-1-5539-en.html
URL: http://ijdld.tums.ac.ir/article-1-5539-en.html
1- Department of Biology, Tehran Azad University, Tehran, Iran
2- Cellular and Molecular Immunology Research Laboratory and Department of Immunology, Dezful University of Medical Sciences, Dezful, Iran ,sheikhi@queensu.ca
3- Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2- Cellular and Molecular Immunology Research Laboratory and Department of Immunology, Dezful University of Medical Sciences, Dezful, Iran ,
3- Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract: (3576 Views)
Background: Usually excess feeding in stored in the body as fat and leads to overweight. One of the causes of catching type 2 diabetes is obesity. Proinflammatory cytokines such as TNF- α and IFNγ can damageTnf- Α And Ifnγ By Peripheral Blood Mononuclear Cells Of Patients With Diabetes Mellitus Type Ii Admitted To Ganjavian Dezful Hospit insulin signaling in insulin-sensitive tissues. In this study, the effect of garlic extract on the expression of TNF-α and IFNγ secreted by the mononuclear cells was measured.
Methods: After taking peripheral blood from patient with type 2 diabetes, mononuclear cells were extracted by Ficoll Method. Cells were a culture with two different concentration of garlic extract (10% and 30%) for 48 and 24 hours. Then for measuring TNF-α and IFNγ release level, ELISA method was used.
Results: Garlic extract can reduce the amount of TNF-α secretion from cells mononuclear, although the timing and concentration of the extract had no effect on the results. Moreover, garlic extract had no effect on the secretion of IFNγ.
Conclusion: Using garlic extract can be adopted therapeutic strategies aimed at adipose tissue inflammation and insulin resistance that is useful for the prevention and treatment of type 2 diabetes.
Methods: After taking peripheral blood from patient with type 2 diabetes, mononuclear cells were extracted by Ficoll Method. Cells were a culture with two different concentration of garlic extract (10% and 30%) for 48 and 24 hours. Then for measuring TNF-α and IFNγ release level, ELISA method was used.
Results: Garlic extract can reduce the amount of TNF-α secretion from cells mononuclear, although the timing and concentration of the extract had no effect on the results. Moreover, garlic extract had no effect on the secretion of IFNγ.
Conclusion: Using garlic extract can be adopted therapeutic strategies aimed at adipose tissue inflammation and insulin resistance that is useful for the prevention and treatment of type 2 diabetes.
Type of Study: Research |
Subject:
Special
Received: 2016/07/30 | Accepted: 2018/01/15 | Published: 2018/03/6
Received: 2016/07/30 | Accepted: 2018/01/15 | Published: 2018/03/6
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