Volume 19, Issue 3 (2-2020)
ijdld 2020, 19(3): 160-169 |
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Fatollahian Z, Monazzami A, Tadibi V, Mostafaei A. MODULATION OF INTERLEUKIN-1Β (IL-1Β ), TUMOR NECROSIS FACTOR–Α (TNF-Α) AND INTERLEUKIN-10 (IL-10 ) GENES EXPRESSION FOLLOWING CONCURRENT TRAINING IN WOMEN WITH TYPE2 DIABETES. ijdld 2020; 19 (3) :160-169
URL: http://ijdld.tums.ac.ir/article-1-5934-en.html
URL: http://ijdld.tums.ac.ir/article-1-5934-en.html
1- Department of Sport Physiology ,Faculty of Sport Science,Razi University, Kermanshah, Iran
2- Department of Sport Physiology ,Faculty of Sport Science,Razi University, Kermanshah, Iran ,monazzami.amirabbas@gmail.com
3- Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
2- Department of Sport Physiology ,Faculty of Sport Science,Razi University, Kermanshah, Iran ,
3- Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract: (2152 Views)
Background: The purpose of this study was to investigate the effect of concurrent training on pre-inflammatory (IL-1β, TNF-α) and anti-inflammatory (IL-10) cytokines gene expression in women with type2 diabetes.
Methods: 18-patients (age30-38, >130 glycemic index) were selected, randomly, and divided into control (n=6) the concurrent training(diabetes, n=6) and the concurrent training (healthy, n=6) groups. The concurrent training protocol consisted of 3 sessions resistance training per week, 8sets with 80% one maximum repetition(
and the endurance training preformed with 30- minutes running (3sets×10 minutes) on a treadmill with 70-80 maximum heart rate(70-80 MHR), immediately. The leukocyte’s IL-1β, TNF-α and IL-10 genes expression determined by the Real time-PCR technique. The quantitative expression of the cytokines gene was calculated using the 2-ΔΔ CT method. The between- groups differences in variables were determined by independent t-test (permutation test) through REST software and independent one-way ANOVA.
Results: The results showed that IL-1β, TNF-α mRNA genes expression reduced significantly after the concurrent training in both the training groups in comparison to the control group (P<0.05). In addition, the results also showed that IL-10 mRNA gene expression was not expressed in leukocytes after the concurrent training in both training groups in comparison to the control group (P>0.05).
Conclusion: In conclusion, the results suggest that the concurrent training modulate IL-1β and TNF-α mRNA genes expression significantly in diabetic women but could not change IL-10 genes expression. This type of exercise training seems to be more effective in reducing the expression of pro-inflammatory cytokines than in enhancing anti-inflammatory cytokines.
Methods: 18-patients (age30-38, >130 glycemic index) were selected, randomly, and divided into control (n=6) the concurrent training(diabetes, n=6) and the concurrent training (healthy, n=6) groups. The concurrent training protocol consisted of 3 sessions resistance training per week, 8sets with 80% one maximum repetition(
and the endurance training preformed with 30- minutes running (3sets×10 minutes) on a treadmill with 70-80 maximum heart rate(70-80 MHR), immediately. The leukocyte’s IL-1β, TNF-α and IL-10 genes expression determined by the Real time-PCR technique. The quantitative expression of the cytokines gene was calculated using the 2-ΔΔ CT method. The between- groups differences in variables were determined by independent t-test (permutation test) through REST software and independent one-way ANOVA.Results: The results showed that IL-1β, TNF-α mRNA genes expression reduced significantly after the concurrent training in both the training groups in comparison to the control group (P<0.05). In addition, the results also showed that IL-10 mRNA gene expression was not expressed in leukocytes after the concurrent training in both training groups in comparison to the control group (P>0.05).
Conclusion: In conclusion, the results suggest that the concurrent training modulate IL-1β and TNF-α mRNA genes expression significantly in diabetic women but could not change IL-10 genes expression. This type of exercise training seems to be more effective in reducing the expression of pro-inflammatory cytokines than in enhancing anti-inflammatory cytokines.
Type of Study: Research |
Subject:
Special
Received: 2020/04/8 | Accepted: 2020/08/19 | Published: 2020/02/29
Received: 2020/04/8 | Accepted: 2020/08/19 | Published: 2020/02/29
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