1- Apadana Institute of Higher Education
2- Apadana Institute of Higher Education ,m.sherafati@apadana.ac.ir
2- Apadana Institute of Higher Education ,
Abstract: (15 Views)
Background: Endoplasmic reticulum stress leads to unfolded or folded protein response, and ATF4 and CHOP proteins play very important roles in this signalling pathway; Therefore, the aim of this research is the effect of resistance training on the content of ATF4 and CHOP proteins in the left ventricle of the heart of type 2 diabetic rats.
Methods: In this experimental study, 12 two-month-old male SpragueDawley rats were selected and their weight reached an average of 280±20 gr after four weeks. Type 2 diabetes was induced by injecting nicotinamide solutions (110 mg/kg) and streptozotocin (60 mg/kg). The rats were randomly divided into 2 groups, resistance training and diabetic control; Resistance training consisted of 8 weeks and 3 sessions per week of climbing a vertical ladder with an 85-degree slope, one meter long with 26 steps and 2 cm space between each step. To analyze data, independent t-test was used in SPSS version 29 and Graphpad Prism version 10.2.3. A significance level of P≤0.05 is considered.
Results: The content of ATF4 and CHOP proteins after 8 weeks of resistance training showed a significant change compared to the control group in the left ventricle of the heart (P=0.001).
Conclusion: The increase of ATF4 and CHOP proteins can lead to increased cell death of cardiomyocytes in the left ventricle of the heart of type 2 diabetic subjects through increasing the endoplasmic reticulum stress and initiating the unfolded or folded protein response.
Methods: In this experimental study, 12 two-month-old male SpragueDawley rats were selected and their weight reached an average of 280±20 gr after four weeks. Type 2 diabetes was induced by injecting nicotinamide solutions (110 mg/kg) and streptozotocin (60 mg/kg). The rats were randomly divided into 2 groups, resistance training and diabetic control; Resistance training consisted of 8 weeks and 3 sessions per week of climbing a vertical ladder with an 85-degree slope, one meter long with 26 steps and 2 cm space between each step. To analyze data, independent t-test was used in SPSS version 29 and Graphpad Prism version 10.2.3. A significance level of P≤0.05 is considered.
Results: The content of ATF4 and CHOP proteins after 8 weeks of resistance training showed a significant change compared to the control group in the left ventricle of the heart (P=0.001).
Conclusion: The increase of ATF4 and CHOP proteins can lead to increased cell death of cardiomyocytes in the left ventricle of the heart of type 2 diabetic subjects through increasing the endoplasmic reticulum stress and initiating the unfolded or folded protein response.
Keywords: Activating Transcription Factor 4, C/EBP Homologous Protein, Resistance Training, Type 2 Diabetes
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