Modulation of oxidative stress through TNFα/SIRT1/NRF1 signaling pathway and kidney function by aerobic exercise with different slopes in chronic kidney disease (CKD) mice.

goli, zahra; taghian, farzaneh; jalali, khosro

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Modulation of oxidative stress through TNFα/SIRT1/NRF1 signaling pathway and kidney function by aerobic exercise with different slopes in chronic kidney disease (CKD) mice.

Zahra Goli¹

, Farzaneh Taghian ^*

², Khosro Jalali¹

1- , Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran,
2- , Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran, , ft.taghian@gmail.com

Abstract: (16 Views)

Introduction:The purpose of the research was to compare the effect of two types of raeobic exercise with and without incline on the modulation of oxidative stress and kidney function in CKD mice.Methods:The number of 24 two-month-old C57bl6 female mice weighing 25±26.1 grams were randomly divided into four groups:healthy control,CKD-affected mice,CKD and aerobic exercise without incline and CKD and aerobic exercise with incline were divided. In order to induce CKD,they received adenine powder of 0.2% mg/kg for 4 weeks.The training groups performed eight weeks of treadmill training(5 sessions perweek)one group performed with a 20-degree incline and the other group performed with a zero-degree incline.After the last training session, sampling of blood was done to measure blood urea nitrogen and urea and glutathione concentration by ELISA method.The expression of SIRT1,NRF1 and TNF-α genes was measured by qPCR-Real Time technique. For statistical analysis,one way ANOVA test and Tukey's post hoc test and significance level P≤0.05 were used.Findings:The results showed that induction of CKD caused a significant increase in blood urea nitrogen and a significant decrease in glutathione concentration compared to the healthy control group (P<0.05).On the other hand, in the exercise group, there is a significant increase in glutathione concentration and a significant decrease in blood urea nitrogen (P<0.05).The expression level of SIRT1 and NRF1 in the slope training group increased significantly and the expression level of TNF-α decreased significantly (P<0.05).

Keywords: chronic kidney disease(CKD), oxidative stress, non-inclined aerobic exercise, aerobic exercise with incline