Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
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Medical Sciences
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2020
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اثر هشت هفته تمرین تناوبی شدید (HIIT) و مصرف کافئین بر سطوح پلاسمایی IL-2 و IL-4 موشهای صحرایی نر دیابتی
EFFECT OF EIGHT-WEEK HIGH INTENSITY INTERVAL TRAINING (HIIT) AND CAFFEINE INTAKE ON SERUM LEVELS OF INTERLEUKIN 2 AND 4 IN MALE DIABETIC WISTAR RATS
عمومى
General
پژوهشي
Research
<strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">مقدمه: </span></span></strong><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">سایتوکاینها از عوامل مرتبط با سیستم ایمنی هستند. تولید سایتوکاینهای پیش التهابی در بیماران دیابتی افزایش مییابد و با انجام تمرینات شدید ورزشی نیز </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تحت تأثیر قرار میگیرد</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">. پژوهش حاضر به بررسی پاسخ</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> <span style="color:black;">شاخص </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">پیش</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">التهابی اینترلوکین 2 </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">(IL<sub>-</sub>2)</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">و</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> <span style="color:black;">شاخص ضدالتهابی اینترلوکین 4 (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IL<sub>-</sub>4</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) به یک دوره تمرین تناوبی شدید (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HIIT</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) و مصرف کافئین </span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">در رتهای دیابتی شده با استرپتوزوتوسین (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">STZ</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) میپردازد.</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"></span></span></span><br>
<strong><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">روشها</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">:</span></span></span></span></strong> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">در یک مطالعهی حیوانی بالینی-مداخلهای</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">50 سر موش صحرایی</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> <span style="color:black;">نر به 5 گروه </span>مساوی </span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">کنترل (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">C</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)، دیابت (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)، دیابت با مکمل (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D+Ca</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)، دیابت با تمرین (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D+T</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)، دیابت با مکمل و تمرین (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D+Ca+T</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)<strong>) </strong></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تقسیم شدند. برنامهی تمرین شامل 8 هفته و هفتهای 5 جلسه (6 تا 12 وهلهی 2 دقیقهای با شدت 90 -85 درصد سرعت ما</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">کس</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">یمم) بود که هر هفته</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">پنج روز </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">mg/kg</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">70 کافئین</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بهصورت هیدراته تزریق شد. 48 ساعت پس از آخرین جلسهی تمرینی، فاکتورهای ایمونولوژیک </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">سرم</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">شامل</span></span></span> <span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IL-2</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IL-4</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> <span style="color:black;">اندازهگیری شد</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ند. تحلیل آماری متغیرها با آزمونهای</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">One-way</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">ANOVA</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Tukey</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> در سطح معناداری</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> (</span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P<0.05</span></span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">.)<span style="color:black;"> انجام گردید.</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"></span></span></span></span><br>
<strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">یافتهها:</span></span></strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> نتایج تحقیق نشان داد که گروه </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D+Ca</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> بهطور معناداری دارای کمترین میانگین غلظت </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IL<sub>2</sub></span></span></span> <span style="font-family:B Lotus;"><span style="font-size:12.0pt;">نسبت به گروه </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> بود (</span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">001/0</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P=</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)</span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">. </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IL-4</span></span></span> <span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بیشترین غلظت را در دو گروه </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D+Ca</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D+Ca+T</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> داشت (</span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">001/0</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P=</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)</span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">. ولی این تفاوتها در دو بین گروه </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D+T</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D+Ca+T</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> نسبت به هم معنادار نبود (</span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">67/0</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P=</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)</span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">.</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"></span></span></span><br>
<strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">نتیجهگیری:</span></span></strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> بهنظر میرسد کافئین بر بهبود وضعیت </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IL-2</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IL-4</span></span></span> <span style="font-family:B Lotus;"><span style="font-size:12.0pt;">در رتهای مبتلابه</span></span> <span style="font-family:B Lotus;"><span style="font-size:12.0pt;">دیــابت ناشی از استرپتوزوتوسین نقشی مؤثر دارد ولی تمرینات</span></span> <span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HIIT</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">در این مورد موثر نیست.</span></span><strong><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"></span></span></span></strong>
<strong>Background:</strong> Cytokines are immune related factors. Production of pro inflammatory cytokines in diabetic patients is increased, which is exacerbated by vigorous training. This study investigates the response of serum IL-2 and IL-4 to eight-week high Intensity Interval Training (HIIT) with caffeine intake on inflammatory and anti-inflammatory responses in diabetic rats.<span dir="RTL"></span><br>
<strong>Methods:</strong> In a clinical-interventional animal study, 50 male rats were randomly assigned to 5 equal groups (control group (C), diabetes group (D), diabetes + caffeine group (D+Ca), diabetes + training group (D+T), diabetes + training + caffeine group (D+Ca+T)) and subjected to 8 weeks of caffeine supplementation (70 mg / kg of caffeine powder was injected five days each week) and 8 weeks of 5 sessions per week with 6 to 12 times, 2-min intervals with intensity of 85-90% of maximal speed. 48 hours after the last training session, immunological factors including IL-2 and IL-4 were measured. Statistical analysis of variables was performed using One-way ANOVA and Tukey tests at the significant level (P<0.05).<br>
<strong>Results:</strong> The results showed that (D+Ca) group had the lowest IL2 in comparison with (D) group (P= 0.001). IL-4 had the highest in (D+Ca) and (D+Ca+T) groups (P = 0.001). But these differences were not statistically significant between (D+Ca) and (D+Ca+T) groups (P= 0.67).<br>
<strong>Conclusion:</strong> It seems that caffeine to play a role in improving the status of IL-2 and IL-4 in streptozotocin-induced diabetes, but HIIT exercises are not effective in this regard.
تمرین تناوبی شدید, کافئین, دیــابت ناشی از استرپتوزوتوسین, اینترلوکین 2, اینترلوکین 4
High Intensity Interval Training (HIIT), Caffeine, Streptozotocin-induced diabetes, Interleukin-2, Interleukin- 4
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