fa تاثیر 8 هفته تمرین ترکیبی بر مقادیر BDNF هیپوکمپ مغز، عملکرد شناختی (Cognitive function)، گلوکز و انسولین رت‌های دیابتی شده با استرپتوزوتوسین تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="tab-stops:right 165.45pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">مقدمه</span></span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">:</span></span></span></b><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">پروتئین<b> </b></span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">BDNF</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> مهم</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">‌</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">ترین نروتروفین است که در عملکرد شناختی نقش دارد<span style="background:white">. </span>دیابت می‌تواند منجر به نقص در عملکرد آن شود و عامل اﺧﺘﻼﻻت ﺷﻨﺎﺧﺘﯽ در ﻫﯿﭙﻮﮐمپ است. هدف از پژوهش حاضر <span style="background:white">بررسی تأثیر 8 هفته تمرین ترکیبی بر مقادیر </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">BDNF</span></span></span></span><span style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black"> هیپوکمپ مغز، عملکرد شناختی (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">Cognitive function</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black">)، گلوکز و انسولین رت‌های دیابتی شده با </span></span></span></span><a name="_Hlk92095801"><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black">استرپتوزوتوسین</span></span></span></span></a> <span lang="FA" style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black">است.</span></span></span></span><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"></span></span></span></b></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 165.45pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">روش‌ها: </span></span></span></b><a name="_Hlk83762234"><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">40 ﺳﺮ رت ﻧﺮ ﻧﮋاد </span></span></span></a><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">اسپراگ‌داولی</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> به‌طور تصادفی به چهار <a name="_Hlk92093960">گروه سالم‌-بی‌تحرک، سالم-تمرین ترکیبی، دیابت-بی‌تحرک و دیابت-تمرین ترکیبی</a> تقسیم شدند.</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">رت‌های گروه‌های تمرین به‌مدت 8 هفته تمرین ترکیبی انجام دادند. پس از 8 هفته،<span style="background:white"> ارزیابی حافظه آزمودنی‌ها با استفاده از تست عملکرد شناختی و در ماز آبی انجام گرفت. سنجش مقادیر </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">BDNF</span></span></span></span><span style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black"> در هیپوکمپ ازطریق آزمایش وسترن بلات انجام شد. برای تجزیه و تحلیل داده‌ها از آنالیز واریانس یکطرفه و آزمون تعقیبی </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">LSD</span></span></span></span><span style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black"> استفاده شد.</span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 165.45pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black">یافته‌ها:</span></span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">مطالعه‌ی حاضر نشان داد که هشت هفته تمرین ترکیبی منجر به کاهش معنی‌‌داری در میزان قندخون ناشتا در گروه تمرین سالم نسبت به گروه بیماردیابتی ش<a name="_Hlk92375893">د (05/0</a></span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P&le;</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">). </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">کاهش غیرمعنی‌داری در میزان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">BDNF</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> هیپوکمپ مغز در گروه تمرین دیابتی نسبت به گروه دیابت نیز مشاهده شد (05/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P&le;</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">). در ضمن، میزان عملکرد حافظه در گروه‌های تمرین سالم و تمرین دیابتی نسبت به سالم بی‌تحرک و دیابتی بی‌تحرک از نظر معنی‌داری در سطح بالاتری قرار داشت (05/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&le;</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">).</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 165.45pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">نتیجه‌گیری: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">تمرین ترکیبی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">BDNF</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> هیپوکمپ مغز را کاهش می‌دهد و</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">میزان عملکرد شناختی در گروه تمرین نسبت به گروه بی‌تحرک افزایش داشت. در نتیجه می‌توان گفت این نوع تمرینات در بهبود عوارض دیابت نوع دو مؤثر است.</span></span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="tab-stops:right 165.45pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Background</span></span></b><b><span new="" roman="" style="font-family:" times=""><span style="color:black">:</span></span></b> <span new="" roman="" style="font-family:" times="">BDNF protein is the important neurotrophin that is involved in cognitive function. Diabetes can lead to impaired function and is a neurocognitive disorder&#39;s factor in hippocampus. Therefore, the purpose of this study was to evaluate the effect of 8 weeks of </span><a name="_Hlk92375520"><span new="" roman="" style="font-family:" times=""><span style="color:black">concurrent</span></span></a><span new="" roman="" style="font-family:" times=""> training on BDNF levels in the brain hippocampus and cognitive function of streptozotocin-induced diabetic rats.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 165.45pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Methods: </span></span></b><span new="" roman="" style="font-family:" times="">40 of Sprague-Dawn rats were randomly divided into four groups: healthy inactive, non-healthy </span><span new="" roman="" style="font-family:" times=""><span style="color:black">concurrent</span></span><span new="" roman="" style="font-family:" times=""> exercise, diabetic group without exercise, and diabetic</span><span new="" roman="" style="font-family:" times=""><span style="color:black"> concurrent</span></span><span new="" roman="" style="font-family:" times=""> exercise. Rats in the training groups performed </span><span new="" roman="" style="font-family:" times=""><span style="color:black">concurrent</span></span><span new="" roman="" style="font-family:" times=""> training for 8 weeks. After 8 weeks, subjects&#39; memory was assessed using a cognitive function test in a water maze. BDNF levels in the hippocampus were measured by Western blotting. One-way analysis of variance and LSD post hoc test were used to analyze the data.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 165.45pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Findings:</span></span></b><span new="" roman="" style="font-family:" times=""> The present study showed that eight weeks of </span><span new="" roman="" style="font-family:" times=""><span style="color:black">concurrent</span></span><span new="" roman="" style="font-family:" times=""> exercise resulted in a significant reduction in fasting blood sugar in the healthy exercise group compared to the diabetic group. A significant decrease in BDNF levels of the</span> <span new="" roman="" style="font-family:" times="">cerebral hippocampus was also observed in the diabetic exercise group compared to the diabetic group. In addition, the level of memory function in healthy exercise and diabetic exercise groups was significantly higher than healthy control and patient control.</span><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 165.45pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion</span></span></b><b><span new="" roman="" style="font-family:" times="">:</span></b> <span new="" roman="" style="font-family:" times=""><span style="color:black">Concurrent</span></span><span new="" roman="" style="font-family:" times=""> exercise reduces BDNF hippocampus and increased cognitive function in the exercise group compared to the control group. As a result, it can be said that this type of exercise is very effective in improving the complications of type 2 diabetes.</span></span></span></span></span> تمرین ترکیبی, پروتئین BDNF, عملکرد شناختی, هیپوکمپ Concurrent exercise, BDNF protein, Cognitive function, Hippocampus 390 403 http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1689-1&slc_lang=fa&sid=1 Mohsen Omidi محسن امیدی 0000-0002-8665-5811 No Department of Exercise Physiology, Faculty of Education and Psychology, Shiraz University, Shiraz, Iran بخش علوم ورزشی، دانشکده‌ی علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران Mohsen Salesi محسن ثالثی mhsnsls@gmail.com 0000-0003-2969-9102 Yes Department of Exercise Physiology, Faculty of Education and Psychology, Shiraz University, Shiraz, Iran بخش علوم ورزشی، دانشکده‌ی علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران Rasoul Rezaei رسول رضایی 0000-0001-5136-3694 No Department of Exercise Physiology, Faculty of Education and Psychology, Shiraz University, Shiraz, Iran بخش علوم ورزشی، دانشکده‌ی علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران Maryam Koushki Jahromi مریم کوشکی جهرمی 0000-0001-9563-9461 No Department of Exercise Physiology, Faculty of Education and Psychology, Shiraz University, Shiraz, Iran بخش علوم ورزشی، دانشکده‌ی علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران