fa تأثیر تمرین مقاومتی و تزریق سلول‌های بنیادی اندوتلیال بر بتا -اکتین، AKT تام و فسفریله در عضلۀ اسکلتی رت‌های دیابتی نوع یک عمومى General پژوهشي Research <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">مقدمه:</span></span></b><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">دیابت نوع یک با کاهش مویرگی عضلۀ اسکلتی و تنظیم نامناسب مسیرهای آنژیوژنز در عضلۀ اسکلتی همراه است. هدف این پژوهش بررسی تأثیر تمرین مقاومتی و تزریق سلول‌های بنیادی اندوتلیال بر بتا-اکتین، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AKT</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> تام و فسفریله در عضلۀ اسکلتی رت‌های دیابتی نوع یک بود. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">روش‌ها:</span></span></b><b> </b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">در این تحقیق تجربی، </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">36 سر رت صحرایی نر نژاد ویستار <a name="_Hlk90067212">(میانگین سن 6 هفته) </a></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">به </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">شش</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">گروه </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">کنترل (سالم)، کنترل </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">دیابتی پایه</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">، کنترل </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">دیابتی</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">، دیابت+تزریق سلول بنیادی، </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">دیابت+تمرین مقاومتی </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">و دیابت+تزریق سلول بنیادی+تمرین مقاومتی تقسیم شدند. در این مطالعه رت‌ها با استفاده از داروی استرپتوزوتوسین به‌صورت تک دوز 40 میلی‌‌گرم به ازای هرکیلوگرم وزن بدن و به‌صورت درون صفاقی دیابتی شدند. تمرینات مقاومتی شامل بالا رفتن از یک نردبان یک متری با وزنۀ آویزان به دم به‌مدت 17 جلسه اجرا گردید. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تعداد 500 هزار سلول بنیادی مشتق از استخوان توسط دستگاه سل کانتر تزریق شد.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">میزان</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بتا-اکتین، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AKT</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> تام و فسفریله </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بافت عضلۀ اسکلتی </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">رت‌ها به روش وسترن بلاتینگ </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">اندازه‌</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">گیری شد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">یافته‌ها:</span></span></b><b> </b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتایج نشان داد تمرین مقاومتی منجر به افزایش معنی‌دار </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Pho-AKT</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&beta;-actin</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">و </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نسبت </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Pho-AKT/AKT</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">کاهش معنی‌دار </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AKT</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بافت عضلانی رت‌های دیابتی نوع 1 شد </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">(001/0></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تزریق سول‌های بنیادی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">منجر به افزایش معنی‌دار </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Pho-AKT</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">و </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نسبت </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Pho-AKT/AKT</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">و </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تمرینات </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">مقاومتی با ترزیق هم‌زمان</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">سول‌های بنیادی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">منجر به افزایش معنی‌دار </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Pho-AKT</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&beta;-actin</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">و </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">کاهش معنی‌دار </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AKT</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بافت عضلانی رت‌های دیابتی نوع یک شد </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">(001/0></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتیجه</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span meiryo="" style="font-family:" ui="">‌</span></span></b><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">گیری: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">با توجه به نتایج،<b> </b>احتمالاً مداخلۀ </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تمرینات </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">مقاومتی با تزریق هم‌زمان</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">سول‌های بنیادی می‌تواند به تنظیم مسیرهای آنژیوژنز عضلۀ اسکلتی </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">در دیابت نوع یک</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">کمک کند. </span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Background: </span></b><span new="" roman="" style="font-family:" times="">Type 1 diabetes is associated with decreased skeletal muscle capillary and improper regulation of angiogenesis pathways in skeletal muscle. This research intended to study the effect of resistance training and endothelial stem cell injection on &beta;eta-actin, phosphorylated and total AKT of skeletal muscle in type 1 diabetic rats.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods: </span></b><span new="" roman="" style="font-family:" times="">In this experimental study, 36 male Wistar rats (age 6 weeks) were divided into six groups of control (healthy), basal diabetic control, diabetic control, diabetes + stem cell injection, diabetes + resistance training and diabetes + stem cell injection + resistance training. In this study, rats became diabetic intraperitoneally using streptozotocin as a single dose of 40 mg/kg. Resistance exercises including climbing a one-meter ladder with weights hanging from the tail were performed for 17 sessions. 500,000 bone-derived stem cells were injected by a cell counter. The levels of &beta;eta-actin, phosphorylated and total AKT in skeletal muscle tissue </span><span new="" roman="" style="font-family:" times="">of </span><span new="" roman="" style="font-family:" times="">rat were measured by using the Western blotting method. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results: </span></b><span new="" roman="" style="font-family:" times="">The results showed that resistance training led to significant increase in Pho-AKT, &beta;-actin and Pho-AKT/AKT ratio and significant decrease in AKT of muscle tissue </span><span new="" roman="" style="font-family:" times="">in</span> <span new="" roman="" style="font-family:" times="">type 1 diabetic rats (P<0.001). Injection of stem cells leads to significant increase in Pho-AKT and Pho-AKT/AKT ratio and resistance training with simultaneous injection of stem cells leads to significant increase in Pho-AKT, &beta;-actin and significant decrease in Akt of muscle tissue </span><span new="" roman="" style="font-family:" times="">in</span> <span new="" roman="" style="font-family:" times="">type 1 diabetic rats (P<0.001).</span><span dir="RTL" lang="AR-SA" style="font-size:13.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion: </span></b><span new="" roman="" style="font-family:" times="">According to the results, it is possible that the intervention of resistance training with injection of stem cells can help regulate the pathways of skeletal muscle angiogenesis in type 1 diabetes.</span><span dir="RTL" lang="AR-SA" style="font-size:13.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span> تمرین مقاومتی, سلول‌های بنیادی, آنژیوژنز, دیابت نوع یک Resistance training, Stem cells, Angiogenesis, Type 1 diabetes 69 80 http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1703-1&slc_lang=fa&sid=1 Elham Mokhtari الهام مختاری 0000-0002-9136-4664 No Department of Physical Education and Sport Science, Karaj Branch, Islamic Azad University, Karaj, Iran گروه فیزیولوژی ورزشی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران Amir Sarshin امیر سرشین Amsarshin@gmail.com 0000-0003-4994-606X Yes Clinical Care and Health promotion Research Center, Karaj branch, Islamic Azad University, Karaj, Iran مرکز تحقیقات مراقبت‌های بالینی و ارتقای سلامت، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران Foad Feizolahi فواد فیض الهی 0000-0003-1847-9745 No Department of Physical Education and Sport Science, Karaj Branch, Islamic Azad University, Karaj, Iran گروه فیزیولوژی ورزشی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران Eidi Alijani عیدی علیجانی 0000-0002-9862-1096 No Department of Physical Education and Sport Science, Karaj Branch, Islamic Azad University, Karaj, Iran گروه فیزیولوژی ورزشی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران