fa تأثیر هشت هفته تمرین هوازی بر بیان پروتئین‌های سالمندی P‌53 و P16 بافت پانکراس موش‌های دیابتی تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">مقدمه</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">: <a name="_Hlk92324933">هایپرگلیسمی مزمن با افزایش آسیب‌های سلولی ناشی از فشار اکسایشی همراه است</a></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">و موجب افزایش مقاومت انسولینی شده و در سلول‌های بتا </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p53</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p16</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> را نیز افزایش می‌دهد که به القای سالمندی در سلول‌های ترشح کننده انسولین بافت پانکراس منجر می‌شود.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">هدف از این پژوهش </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تأثیر هشت هفته تمرین هوازی بر بیان پروتئین‌های سالمندی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P53</span></span> <span style="font-size:12.0pt"><span b="" lotus="" style="font-family:">&nbsp;و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P16</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> بافت پانکراس موش‌های دیابتی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بود</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">روش‌ها</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">: </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">در این مطالعه، تعداد 15 سر موش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NMRI</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">(22/3 </span></span><span lang="AR-SA" style="font-size:12.0pt">&plusmn;</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> 26 گرم) به‌صورت تصادفی در سه گروه کنترل سالم، کنترل دیابتی و تمرینی دیابتی قرار گرفتند. سپس از راه غذای پُرچرب</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">به‌مدت 5 هفته و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تزریق درون صفاقی استرپتوزوسین دیابتی شدند. پروتکل تمرین </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">هوازی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Vmax</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">60-50%</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)، 5 روز در هفته به‌مدت 8 هفته بود. پس از بی‌هوشی، خون و بافت پانکراس برداشته شد. میزان مقاومت انسولینی، غلظت پروتئین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P53</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P16</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> در </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">سلول‌های بتای پانکراس </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">اندازه‌گیری شد. داده‌ها به روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ANOVA</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">با</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">سطح معنی‌داری 05/0 ارزیابی شدند</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">یافته‌ها</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">: </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">برطبق نتایج هشت هفته تمرین هوازی در موش‌های مبتلا به دیابت نوع دو، </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">کاهش</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">معنی‌دار مقاومت انسولینی (005/0</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)، غلظت پروتئین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P53</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> (</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">002/0=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P16</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> (</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">010/0=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) را در بافت پانکراس به</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">دنبال دارد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتیجه‌گیری</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">: </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تمرین هوازی احتمالاً با کاهش عوامل مربوط به سالمندی سلولی نظیر </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P53</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">&nbsp; و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P16</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> در سلول</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">های بتا موجب </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بهتر شدن</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">حساسیت انسولینی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">و به تأخیر افتادن سالمندی سلولی ناشی از دیابت می‌شود. لذا می‌توان به این نوع فعالیت ورزشی به‌عنوان یک رویکرد درمانی غیرتهاجمی جهت بهبود شرایط این بیماران نگریست. </span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">Background:</span></span></b> <span new="" roman="" style="font-family:" times="">Chronic hyperglycemia is associated with an increase in cellular damage due to oxidative stress</span> <span new="" roman="" style="font-family:" times="">and increases insulin resistance and also increases in p53 and p16 beta cells, leading to the induction of senescence in pancreatic insulin-secreting cells. The aim of this study was the effect of eight weeks of aerobic exercise on the expression of senescence proteins P53 and P16 in the pancreatic tissue of diabetic mice.</span><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In this study, 15 NMRI mice (26.3 &plusmn;3.22 g) were divided into three groups randomly: healthy control, diabetic control and diabetic exercise. They were diabetic by HFD for 5 weeks and intraperitoneal injection of STZ. The </span><span new="" roman="" style="font-family:" times="">aerobic </span><span new="" roman="" style="font-family:" times="">training protocol (50-60% Vmax) was 5 days a week for 8 weeks. After anesthesia, blood and pancreatic tissue were removed. Insulin resistance, P53 and P16 protein concentrations in pancreatic beta cells were measured. Data were analyzed by </span><span new="" roman="" style="font-family:" times="">ANOVA </span><span new="" roman="" style="font-family:" times="">with a significance level of p <0.05.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><span new="" roman="" style="font-family:" times=""> According to the results of eight weeks of aerobic exercise by mice diabetic type 2, a significant decrease in insulin resistance (p = 0.005), protein concentrations of P53 (p = 0.002) and P16 (p = 0.010) in pancreatic tissue was observed.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><span new="" roman="" style="font-family:" times=""> Aerobic exercise may improve insulin sensitivity and delay cellular senescence due to diabetes by reducing cell senescence factors such as P53 and P16 in beta cells. Therefore, this type of exercise can be considered as a therapeutic approach to improve the condition of these patients.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><span style="font-family:"Times New Roman",serif" lang="EN"></span></span></span></span> تمرین هوازی, سالمندی سلولی, P53 , P16 Aerobic Exercise, Cellular Senescence, p53 protein, p16 protein 45 54 http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1729-1&slc_lang=fa&sid=1 Maryam Janbozorgi مریم جان بزرگی 0000-0002-3614-727x No Department of Sport Physiology, University of Tehran, Tehran, Iran گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران Abass ali Gaini عباسعلی گایینی aagaeini@ut.ac.ir 0000-0002-8679-0669 Yes Department of Sport Physiology, University of Tehran, Tehran, Iran گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران Siroos Choobineh سیروس چوبینه 0000-0003-4466-0986 No Department of Sport Physiology, University of Tehran, Tehran, Iran گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران Mohamad reza Tabandeh محمدرضا تابنده 0000-0003-3258-8550 No Department of basic sciences, division of biochemistry and molecular biology, faculty of veterinary medicine, shahid chamran university of ahvaz, ahvaz, Iran گروه علوم پایه، دانشکده‌ی دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران