Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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admin
2345-4008
2345-4016
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fa
jalali
1401
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gregorian
2022
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online
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fulltext
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تأثیر همزمان تمرینهای مقاومتی و تزریق سلولهای اجدادی اندوتلیال بر سطوح ایمونوگلوبولینها (IgA, IgM, IgG) در خون رتهای دیابتی القاء شده با استرپتوزوتوسین
Simultaneous Effect of Resistance Training and Endothelial Progenitor Cell Injection on Immunoglobulins (Iga, Igm, Igg) In Muscle Tissue of Streptozotocin-Induced Diabetic Rat
تخصصي
Special
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">دیابت بهعنوان یک بیماری پیشرونده، میتواند منجر به کاهش عملکرد دستگاه ایمنی شود. از اینرو؛ هدف پژوهش حاضر تعیین اثر همزمان تمرینهای مقاومتی و تزریق سلولهای اجدادی اندوتلیال بر ایمونوگلوبولینها </span></span>(IgA, IgM, IgG)<span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> رتهای دیابتی القاء شده با استرپتوزوتوسین انجام بود. </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">روشها: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">30 </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">سر</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">موش</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">صحرایی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">(با سن 6 هفته و میانگین وزنی 20</span></span><b>±</b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">200گرم)، بهصورت تصادفی در گروههایی شامل؛ دیابت+تزریق<sub> </sub>سلولهای بنیادی+تمرین مقاومتی (6=</span></span>n<span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)، دیابت+تمرین مقاومتی (6=</span></span>n<span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)، دیابت+تزریق سلول بنیادی (6=</span></span>n<span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)، دیابتی کنترل (6=</span></span>n<span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) و سالم پایه (6=</span></span>n<span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) تقسیم شدند. جهت </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بررسی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تغییرات ایمونوگلوبینها از روش وسترن بلات استفاده شد. همچنین، </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">برای مقایسه بین و درون گروهی از آزمون آنالیز واریانس دوراهه استفاده شد و برای درک بهتر نتایج نیز، اندازۀ اثر و مقدار فاصلۀ اطمینان 95% آورده شد. </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">یافتهها: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتایج نشان داد </span></span>IgA<span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> (022/0</span></span>P=<span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)، </span></span>IgM<span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> (017/0</span></span>P=<span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)، </span></span>IgG<span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> (045/0</span></span>P=<span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) بین گروهها تغییرات معنیداری داشت. همچنین، اختلاف معنیداری در هر سه متغیر بین گروه دیابت کنترل و گروه دیابت+تمرین مقاومتی+تزریق وجود داشت </span></span>(P≤0/05)<span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">. </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتیجهگیری: </span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">با جمعبندی نتایج تحقیق حاضر احتمالاً میتوان گفت تمرینهای مقاومتی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تزریق</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">همزمان</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">سلولهای</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">اجدادی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">اندوتلیال</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">موجب بهبود وضعیت ایمونوگلوبینها </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">در</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">اثر</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تمرین</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تزریق</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">میشود.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">این</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">یافتهها</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">ادعا</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">میکند</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">که</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تمرین</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">مقاومتی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تزریق</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">میتواند</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بهعنوان</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">یک</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">روش</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">درمانی، موجب بهبود عملکرد دستگاه ایمنی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">ناشی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">از</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بیماری</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">دیابت</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بهکار</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">رود</span></span>.<span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> </span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background:</span></b> <span lang="EN" style="font-size:11.0pt">Diabetes, as a progressive disease, can lead to decreased immune function. therefore, the aim of this study was to determine the simultaneous effect of resistance training and endothelial progenitor cell injection on immunoglobulins (IgA, IgM, IgG) of streptozotocin-induced diabetic rats.</span><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Methods</span></b><span style="font-size:11.0pt"> 30 rats (aged 6 weeks with a mean weight of 200±20 g) were randomly divided into groups including Diabetes + stem cell injection + resistance training (n = 6), diabetes + resistance training (n = 6), diabetes + stem cell injection (n = 6), control diabetes (n = 6) and healthy basal (n = 6) Were divided. Western blotting was used to evaluate the changes in immunoglobulins. Also, two-way analysis of variance was used for comparison between and within the group, and for better understanding of the results, the effect size, and the amount of 95% confidence interval were given.</span><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results: </span></b><span lang="EN" style="font-size:11.0pt">The results showed that IgA (P = 0.022), IgM (P = 0.017), IgG (P = 0.045) had significant changes between groups. Also, there was a significant difference in all three variables between the control diabetes group and the diabetes + resistance training + injection group (P≤0.05)</span><span style="font-size:11.0pt">.</span><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion:</span></b> <span lang="EN" style="font-size:11.0pt">Summarizing the results of the present study, it can probably be said that resistance training and simultaneous injection of endothelial progenitor cells improve the status of immunoglobulins by training and injection. These findings suggest that resistance training and injections can be used as a treatment to improve the function of the immune system due to diabetes.</span><b><span style="font-size:11.0pt"></span></b></span></span></span>
دیابت نوع یک, تمرینهای مقاومتی, سلولهای اجدادی اندوتلیال, سلول درمانی, ایمونوگلوبینها
Type 1 diabetes, Resistance training, Endothelial progenitor cells, Cell therapy, Immunoglobulins
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1733-1&slc_lang=fa&sid=1
Suren
Valafar
سورن
والافر