Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
000
000
000
000
000
fa
jalali
1401
4
1
gregorian
2022
7
1
22
2
online
1
fulltext
fa
تأثیر هشت هفته تمرین استقامتی، بر میزان GLUT4 میوکارد و آنزیمهای کبدی در موشهای دیابتی نوع دو
The Effect of 8 Weeks Endurance Training on Myocardial GLUT4 and Liver Enzymes in Type 2 Diabetic Rats
تخصصي
Special
كاربردي
Applicable
<span style="font-size:14pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span style="font-style:italic"><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-style:normal">مقدمه:</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> دیابت نوع دو طی سالها علاوه بر این که عوارض جبران ناپذیری در قسمتهای مختلف بدن میگذارد موجب اختلال در عملکرد کبد میشود و خطر افزایش نارسایی قلب را افزایش میدهد ولی بهدلیل عدم بروز علایم ظاهری، کمتر مورد توجه قرار گرفته است، بنابراین هدف از پژوهش حاضر بررسی تأثیر هشت هفته تمرین استقامتی، بر شاخصهای دیابتی و میزان </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">GLUT4</span></span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> قلبی و آنزیمهای کبدی موشهای صحرایی دیابتی نوع دو بود.</span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:14pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span style="font-style:italic"><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-style:normal">روشها:</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> بدین منظور تعداد 24 سر موش صحرایی ویستار (وزن اولیه 20</span></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span calibri="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">±</span></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">220 گرم) به سه گروه هشت تایی تقسیم شدند: گروه اول: کنترل سالم، گروه دوم: دیابتی، گروه سوم: تمرین استقامتی دیابتی. جهت القای دیابت نوع دو ابتدا دو هفته غذای پُرچرب به موشهای صحرایی داده شد سپس استرپتوزوتوسین (تهیه شده از شرکت سیگما آلدریچ) با دوز </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">mg/kg</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">37 در بافر سیترات </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">M</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">1/0 (</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">PH</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">5/4) بعد از شش ساعت ناشتایی بهصورت درون صفاقی تزریق شد برای گروه کنترل سالم همان میزان، بافر تزریق شد، همچنین گروههای تمرین هوازی پنج جلسه در هفته و هشت هفته بر روی نوار گردان موتوردار دویدند. سپس شاخصهای دیابتی، </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">GLUT4</span></span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> میوکارد (وسترن بلات) و آنزیمهای کبدی (الایزا) موشهای صحرایی اندازهگیری شد. </span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:14pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span style="font-style:italic"><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-style:normal">یافتهها:</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> تمرین استقامتی موجب کاهش معنیدار گلوکز خون ناشتا، شاخص مقاومت به انسولین (هر دو 00/0=</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">P</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">) شد. همچنین موجب افزایش معنیدار انسولین (01/0=</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">P</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">) و شاخص حساسیت انسولینی (00/0=</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">P</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">) نسبت به گروه کنترل دیابتی شد. از طرفی میزان </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">GLUT4</span></span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> در گروه دیابتی تمرین افزایش معنی</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">داری داشت (01/0=</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">P</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">)</span></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">.</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> همچنین نتایج نشان داد در مقایسه با گروه کنترل دیابتی، </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">ALT</span></span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> و </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">AST</span></span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> در دیابتی</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">ها با تمرین استقامتی افزایش معنی</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">داری نداشتند (به</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">ترتیب 30/0=</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">P</span></span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> ، 0 5/0=</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">P</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">).</span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:14pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span style="font-style:italic"><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-style:normal">نتیجهگیری:</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> براساس نتایج بهدستآمده به نظر میرسد که تمرین استقامتی ضمن افزایش معنیدار میزان </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">GLUT4</span></span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> قلب موجب کاهش معنیدار شاخصهای دیابتی ازجمله گلوکز ناشتا، شاخص </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal">HOMA-IR</span></span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"> و نیز آنزیمهای کبدی میشود.</span></span></span></span></span></span></span></span></span></span></span>
<span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background:</span></b><span style="font-size:11.0pt"> Over the years type 2 diabetes, in addition to causing irreversible effects on various parts of the body, also causes liver dysfunction and increases the risk of heart failure, but due to the lack of physical symptoms, less attention has been paid. So, the aim of this study was to determine the effect of 8 weeks of endurance training on diabetic indexes and myocardial GLUT4 and liver enzymes of type 2 diabetic rats.</span></span></span><br>
<span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Methods:</span></b><span style="font-size:11.0pt"> A total number of 24 rats (220±20) were divided randomly into three groups; 1-Non-diabetic Control group (NC, n=8). 2-Diabetic Control group (D, n=8) To induce type 2 diabetes, high-fat diets were given to rats for two weeks, then streptozotocin (Aldrich company) at a dose of 37 mg / kg in citrate buffer M0.1 (PH4.5) after 6 hours of intraperitoneal fasting was injected, For the healthy control group, the same amount of buffer was injected. Also, aerobic exercise groups ran 5 times a week and 8 weeks on a motorized treadmill. Diabetic index’s, myocardial GLUT4 (Western blotting) and liver enzymes (ELISA) of rats were measured. </span></span></span><br>
<span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results:</span></b><span style="font-size:11.0pt"> Endurance training significantly decreased fasting blood glucose, insulin resistance index (both P= 0.00). It also caused a significant increase in insulin (P= 0.01) and insulin sensitivity index (P= 0.00) compared to the diabetic control group. On the other hand, the amount of GLUT4 in the training group increased significantly (P= 0.01). The results also showed that in comparison with the diabetic control group, ALT and AST did not increase significantly in diabetics with endurance training (P= 0.30, P= 0.5, respectively). </span></span></span><br>
<span style="font-size:9pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion:</span></b><span style="font-size:11.0pt"> Based on the results, it seems that endurance training significantly increases the level of GLUT4 in the heart and significantly decreased diabetic parameters such as fasting glucose, HOMA-IR index and liver enzymes.</span></span></span>
تمرین استقامتی, شاخص دیابتی, GLUT4, آنزیمهای کبدی, دیابت نوع دو
Endurance training, diabetic indexes, GLUT4, Liver enzymes, Type II diabetes
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-395-2&slc_lang=fa&sid=1
Mahdi
Faramoushi
مهدی
فراموشی
m.faramoushi@tabriziau.ac.ir