Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
000
000
000
000
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fa
jalali
1401
4
1
gregorian
2022
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22
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online
1
fulltext
fa
تأثیر تمرینات تناوبی شدید بر بیان ژن FTO در بافت چربی زیر پوستی موشهای صحرایی دیابتی نژاد ویستار
Effect of High Intensity Interval Training on FTO Gene Expression in Subcutaneous Fatty Tissue of Diabetic Wistar Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> ژن مرتبط با تودۀ چربی و چاقی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">FTO</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) با خطر افزایش چاقی و دیابت نوع دو ارتباط مستقیم دارد. هدف این مطالعه بررسی تأثیر 12 هفته تمرین تناوبی شدید بر بیان ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">FTO</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در بافت چربی زیر پوستی موشهای صحرایی دیابتی نوع دو بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">روشها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در این مطالعۀ تجربی 12 سر موش صحرایی نر نژاد ویستار با وزن </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">10</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="color:black">±</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">220 گرم با تزریق </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">نیکوتین آمید-استرپتوزوتوسین به دیابت نوع دو مبتلا شدند و تصا</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">د</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">فی در دو گروه تمرین (6</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">n=</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">)</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">و کنترل (6</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">n=</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">قرار گرفتند. گروه تمرین، برنامۀ تمرینات تناوبی شدید را </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">بهمدت 12 هفته و 5 روز در هفته با دویدن روی تردمیل اجرا کردند.</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">48 ساعت پس از آخرین جلسۀ تمرینی، بیان ژن</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">FTO</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در بافت چربی زیر پوستی،</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">سطح </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">گلوکز</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">خون، </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">انسولین و مقاومت انسولین در هر دو گروه اندازهگیری شدند. تجزیه و تحلیل دادهها با استفاده از آزمون تی مستقل در سطح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P<0.05</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> انجام شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">یافتهها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> پس از مداخلۀ ورزشی، سطح گلوکز خون، مقاومت انسولین و بیان ژن</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">FTO</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">بافت چربی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">در گروه تمرین نسبت به گروه کنترل به میزان معنیداری کاهش یافتند (05/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P<</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">). سطح سرمی انسولین در گروه تمرین نسبت به گروه کنترل به میزان معنیداری افزایش یافت (05/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P<</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">). </span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">نتیجهگیری:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> بهنظر میرسد تمرین تناوبی شدید میتواند سبب </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">کاهش گلوکز خون، مقاومت انسولین و </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">بیان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">FTO</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در بافت چربی رتهای دیابتی نوع دو شود. بهبود کنترل گلیسمیک در رتهای دیابتی ممکن است به کاهش </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">بیان ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">FTO</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در پاسخ به تمرینات تناوبی شدید نسبت داد</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">ه شود</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">. </span></span></span></span></span></span></span></span>
<span style="font-size:12pt"><span style="font-family:" times=""><span style="color:black"><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background:</span></span></span></b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Fat mass and obesity-associated gene (FTO) <em>is directly associated</em><i> </i>with increased<i> <em>risk of obesity and </em></i>type 2 diabetes mellitus (T2DM)<em>. </em>The purpose of this study was to investigate the effect of 12 weeks of high intensity interval training (HIIT) on FTO expression of subcutaneous fat tissue in T2DM rats.</span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="font-family:" times=""><span style="color:black"><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Methods:</span></span></span></b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> In this experimental study, twelve males Wistar rats (220±10 g) with T2DM induced by streptozotocin-nicotinamide injection were randomly divided into exercise (n=6) and control (n=6) groups. The</span></span></span> <span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">training group performed the HIIT protocol on a treadmill for 12 weeks and 5 days per week. FTO expression in subcutaneous fatty tissue, blood glucose level, insulin and insulin resistance were measured 48 hours after the last training session. Data were analyzed by independent T test </span></span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">at </span></span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P< 0.05.</span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="font-family:" times=""><span style="color:black"><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Results:</span></span></span></b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Blood glucose level, insulin resistance and FTO expression in fat tissue decreased significantly in training groups compared to control group after the exercise intervention (P˂0.05). Serum insulin increased significantly in training groups compared to control group (P˂0.05).</span></span></span><span dir="RTL" lang="FA" style="font-size:14.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-autospace:none"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion: </span></span></b><span new="" roman="" style="font-family:" times=""><span style="color:black">It seems that HIIT can decrease glucose, insulin resistance and FTO expression of fat tissue of T2DM rats. Improved glycemic control in diabetic rats might be attributed to reduced FTO expression in response to HIIT.</span></span><span dir="RTL" lang="FA" style="font-size:14.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span>
تمرین تناوبی, دیابت نوع دو, مقاومت انسولین, ژن FTO
Interval training, Type 2 diabetes, Insulin resistance, FTO gene
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81
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1681-1&slc_lang=fa&sid=1
Porya
Mohammadiyan
پوریا
محمدیان