Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
000
000
000
000
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fa
jalali
1401
6
1
gregorian
2022
9
1
22
3
online
1
fulltext
fa
تأثیر همزمان تمرینات مقاومتی و تزریق سلولهای بنیادی برسطوح گلوکز خون و بیان پروتیین Bax و Bcl2 از شاخصهای آپوپتوز عضلهی اسکلتی در رتهای نر دیابتی القاء شده با STZ
Simultaneous Effect of Resistance Training and Stem Cell Injection on Blood Glucose Levels and Bax and Bcl2 Protein Expression from Markers of Skeletal Muscle Apoptosis in STZ-Induced Diabetic Male Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">مقدمه: </span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">دیابت نوع یک اختلالی است که بر اثر تخریب خود ایمنی سلولهای مولد انسولین پانکراس بهوجود میآید. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">پروتئینهای </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bax</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bcl2</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> نقش مهمی در ایجاد فرایند آپوپتوز دارد.</span></span> <b><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">روشها</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">: </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">در پژوهش حاضر </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تعداد 30 سر رت نر نژاد </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">ویستار از میان رتهای موجود (500 رت) در آزمایشگاه با وزن تقریبی 20</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">±</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> 200 بهطور تصادفی انتخاب شدند.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">آزمودنیها پس از 2 هفته آشنایی با محیط </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">به 5 گروه 6 تایی شامل (دیابت + تزریق، تمرین مقاومتی) و (دیابت + تمرین مقاومتی) و (دیابت +تزریق) و (دیابتی کنترل به جهت کنترل گذرزمان) و (دیابتی سالم) در شرایط یکسان آزمایشگاهی تقسیم شدند و توسـط تزریق درون صفاقی استرپتوزوتوسین (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">stz</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">mg/kg</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> 60) به دیابت نوع یک مبتلا شدند. موشهای صحرایی گروه دیابتی و گروه دیابت + تزریق سلولهای بنیادی بهمدت 5 هفته در مجموع 17 جلسهی تمرین مقاومتی داشتند. تحقیق حاضر از نوع بنیادی و به روش تجربی انجام شد. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">۴۸ </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">ساعت پس از آخرین جلسهی تمرین سطح گلوکز خون و میزان بیان</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">پروتیین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bax</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bcl2</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">در بافت عضلهی به روش الایزا اندازهگیری شد. </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">یافتهها: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتایج تحقیق حاضر نشان داد که بین میانگین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bax</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bcl2</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> گروه تمرین مقاومتی با تزریق همزمان سلولهای بنیادی و گروه تمرین تفاوت معنیداری مشاهده نشد.<b> </b></span></span><b><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتیجهگیری: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتایج تحقیق حاضر نشان داد که اجرای 17 جلسهی تمرین مقاومتی به تنهایی و به همراه تزریق سلولهای بنیادی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بر میزان بیان</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">پروتیین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bax</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bcl2</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> مؤثر بود، اما تمرین به تنهایی و تمرین به همراه سلولهای بنیادی تأثیر معنیداری بر بیان فاکتورهای نامبرده نداشت.</span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Background:</span></b><span new="" roman="" style="font-family:" times=""> Type 1 diabetes is a disorder caused by autoimmune destruction of pancreatic insulin-producing cells. This induction of autoimmunity may be due to genetic and environmental factors. Bax and Bcl2 proteins play an important role in the process of apoptosis. </span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In this study, 30 male Wistar rats weighting approximately 200±20gr were randomly selected from available rats in lab (500). Subjects after 2 weeks of familiarity with the environment were randomly divided into 5 groups of 6, including (diabetes + injection, exercise) and (diabetes + exercise) and (diabetes + injection) and (diabetes control to control the passage of time) and (basic diabetes to Defaults) under the same laboratory conditions and developed type 1 diabetes by intraperitoneal injection of streptozotocin (stz) (60 mg / kg). Rats in the diabetic group and the diabetic group + stem cell injection had a total of 17 sessions of resistance training for 5 weeks. </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><span new="" roman="" style="font-family:" times=""> The results of the present study showed that there was no significant difference between the mean of Bax and Bcl2 in the resistance training group with simultaneous injection of stem cells and the training group.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><span new="" roman="" style="font-family:" times=""> The results of our study showed that performing 17 sessions of resistance training alone with stem cell injection was effective on the expression of Bax protein and Bcl2, but there was no significant difference between the effect of training alone and training with stem cells. </span></span></span></span>
آپوپتوز, دیابت نوع یک, سلولهای بنیادی, تمرین مقاومتی, 2 Bax, Bcl
Apoptosis, Type 1 diabetes, Stem cells, Resistance training, Bax, Bcl2
185
198
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-360-4&slc_lang=fa&sid=1
Masomeh
Sarmadiyan
معصومه
سرمدیان