Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
000
000
000
000
000
fa
jalali
1401
6
1
gregorian
2022
9
1
22
3
online
1
fulltext
fa
تأثیر هشت هفته تمرین تناوبی شدید بر بیان پروتئینهای آنژیواستاتین و کیناز چسبندگی کانونی بطن چپ قلب رتهای مدل دیابتی
The Effect of Eight- Week High-Intensity Interval Training on the Expression of Cardiac Angiostatin and Focal adhesion Kinase Proteins at Left Ventricle in Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> دیابت با تغییر تعادل بین عوامل تحریک کننده و مهار کنندهی آنژیوژنز میتواند باعث افزایش بیماریهای قلبی و عروقی شود. در این مطالعه اثر هشت هفته تمرین تناوبی شدید (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">HIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) بر بیان پروتئین آنژیواستاتین و</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">کیناز چسبندگی کانونی(</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">FAK-1</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) بطن چپ قلب رتهای دیابتی بررسی شد. </span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">روشها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در این مطالعهی تجربی، تعداد 24 سر رت نر ویستار بهطور تصادفی به سه گروه مساوی (8=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">n</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">): کنترل-سالم (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">C</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">)، دیابت (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">D</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) و دیابت-تمرین (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">D+T</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) تقسیم شدند. دیابت با تزریق استرپتوزوتوسین در رتهای نر القا شد. گروه تمرین بهمدت هشت هفته برنامهی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">HIIT</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> را روی تردمیل انجام دادند. در پایان هفتهی هشتم نمونه بافت بطن چپ </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">قلب</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">جمعآوری شد. دادهها با استفاده از آزمون آماری </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ANOVA</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> و آزمون تعقیبی توکی در سطح معنیداری 05/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P<</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> تحلیل شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">یافتهها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> نتایج نشان داد که القای دیابت باعث افزایش بیان آنژیواستاتین (006/0=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">)، گلوکز سرمی (001/0=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) و کاهش بیان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">FAK-1</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> (001/0=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) شد. </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">HIIT</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در رتهای دیابتی باعث کاهش معنیداری در بیان آنژیواستاتین (006/0=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">p</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) و میزان گلوکز (001/0=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) شد. همچنین افزایش معنیداری در بیان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">FAK-1</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">D+T</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> مشاهده شد (001/0=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">). </span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">نتیجهگیری:</span></span></span></b> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">HIIT</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> باعث کاهش در بیان پروتئین آنژیواستاتین و میزان گلوکز و همچنین افزایش بیان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">FAK-1</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> بطن چپ قلب شد. بهنظرمیرسد تمرین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">HIIT</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> با تغییر در این شاخصهای مؤثر بر رگزایی و گلوکز خون میتواند عوارض قلبی عروقی ناشی از دیابت را در رتهای نر کاهش دهد. </span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Background: </span></span></b><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">Diabetes can increase cardiovascular disease by altering the equilibrium between angiogenic stimulants and inhibitors of angiogenesis. In this study, we investigated the effect of High-Intensity Interval Training (HIIT) on angiostatin </span></span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">and focal adhesion kinase<b><span style="background:white"> (</span></b><span style="background:white">FAK-1) in left ventricle cardiomyocytes in diabetic rats.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Methods:</span></span></b><span new="" roman="" style="font-family:" times=""><span style="color:black"> <span style="background:white">In this experimental study, 24 male</span> Wistar rats were randomly divided into three equal groups (n=8): Control-Normal (C), Diabetes (D), and Diabetes-training (D+T). Diabetes was induced by the injection of streptozotocin in male rats. The Training group has performed a HIIT program on a motor-driven treadmill for eight weeks. At the end of eight weeks-- heart tissue samples were collected. Data were analyzed using ANOVA and Tukey post hoc at P<0.05.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results: </span></b><span new="" roman="" style="font-family:" times="">The results showed that induction of diabetes increased the expression of angiostatin (P=0.006), and serum glucose (P=0.001) and decreased FAK-1 (P=0.001). HIIT significantly reduced the level of angiostatin (P=0.006) and glucose (P=0.001) in diabetic rats. There was also a significant increase in FAK-1 in the D+T group (P=0.001).</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><span new="" roman="" style="font-family:" times=""> HIIT decreased the protein expression of angiostatin and glucose and increased FAK-1 in cardiomyocytes. HIIT seems to reduce the vascular complications of diabetes in rats by altering these parameters that affect angiogenesis and blood glucose.</span></span></span></span>
فعالیت ورزشی, آنژیواستاتیک, آنژیوژنیک, دیابت
Exercise, Angiostatic, Angiogenic, Diabetes
133
144
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1328-6&slc_lang=fa&sid=1
Ghasem
Torabi Palat Kaleh
قاسم
ترابی پلت کله