fa تأثیر تمرین تناوبی طولانی‌‌مدت شدید بر محتوای درون سلولی پروتئین‌های MAFbx و MuRF1 در بطن چپ قلب رت‌های مبتلا به دیابت نوع دو عمومى General پژوهشي Research <span style="font-size:11pt"><span style="tab-stops:right 319.15pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">مقدمه:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">پروتئین‌های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MAFbx</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MuRF1</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">عوامل مهم در مسیر </span></span></span><em><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" lotus="" style="font-family:">یوبی‌کوئیتین و مسئول آتروفی عضلانی</span></span></span></em> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">هستند. </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">هدف از این مطالعه، بررسی </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تأثیر تمرین تناوبی طولانی‌‌مدت پُرشدت (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) بر محتوای درون سلولی پروتئین‌های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MAFbx</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MuRF1</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> در بطن چپ قلب رت‌های مبتلا به دیابت نوع دو </span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">است.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 319.15pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">روش‌ها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> در این مطالعه‌ی تجربی، 18 سر رت نر 2 ماهه از نژاد اسپراگ‌داولی با میانگین وزن 20</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="letter-spacing:-.2pt">&plusmn;</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">270 گرم انتخاب شدند. 12 سر از رت‌ها از طریق تزریق درون صفاقی محلول‌های </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">استرپتوزوتوسین <span style="letter-spacing:-.2pt">و نیکوتین‌آمید دیابتی شدند. این </span>رت‌ها <span style="letter-spacing:-.2pt">به روش تصادفی به 2 گروه، تمرین دیابتی و کنترل دیابتی (هر گروه 6 سر) تقسیم شدند؛ یک گروه کنترل سالم (6 سر) نیز در نظر گرفته شد. گروه تمرینی 4 روز در هفته، به‌مدت 8 هفته به </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">HIIT</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> پرداختند</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">.</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> برای تجزیه‌و‌تحلیل داده‌ها از نرم‌افزار </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">SPSS</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> نسخه‌ی 23 و </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">آزمون‌های آنوای یک‌طرفه و تعقیبی توکی استفاده&rlm; شد.</span></span></span><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></b></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:dotted 0cm right blank 319.15pt"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">یافته‌ها: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">محتوای پروتئین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MAFbx</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> به‌دنبال 8 هفته </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">، کاهش معنی‌داری را نشان داد (0001/0</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)؛ آزمون تعقیبی توکی نشان داد این تغییر معنی‌دار بین جفت گروه‌های تمرین دیابتی و کنترل دیابتی و همچنین بین جفت گروه‌های کنترل دیابتی و کنترل سالم است (0001/0</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">). محتوای پروتئین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MuRF1</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> کاهش معنی‌داری را نشان داد (0001/0</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)؛ این تغییر معنی‌دار بین جفت گروه‌های تمرین دیابتی و کنترل دیابتی، گروه‌های تمرین دیابتی و کنترل سالم و همچنین گروه‌های کنترل دیابتی و کنترل سالم بود (0001/0</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">).</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:dotted 0cm right blank 319.15pt"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">نتیجه‌گیری: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">به ‌نظر می‌رسد</span></span></span><b> </b><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">HIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">، با کاهش محتوای </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">پروتئین‌های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MAFbx</span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MuRF1</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">در قلب آزمودنی‌های دیابتی روند آتروفی و اتوفاژی کاردیومیوست‌های را مهار کند.</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="tab-stops:right 319.15pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Background: </span></b><span new="" roman="" style="font-family:" times="">MAFbx and MuRF1 proteins are important factors in the ubiquitin pathway and are responsible for muscle atrophy. The purpose of this study was to investigate </span><span new="" roman="" style="font-family:" times="">the effect of long-term </span><em><span style="background:white">high-intensity interval training (HIIT)</span></em><span new="" roman="" style="font-family:" times=""> on the intracellular content of MAFbx and MuRF1 proteins in the left ventricular of the heart of rats with type 2 diabetes.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 319.15pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.65pt">Methods:</span></span></b><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.65pt"> In this experimental study, 18 rats 2-month-old male Sprague-Dawley rats with a mean weight of 270&plusmn;20 g were selected. 12 rats became diabetic by intraperitoneal injection of </span></span><span new="" roman="" style="font-family:" times="">Streptozotocin</span><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.65pt"> and nicotinamide solutions. These rats were randomly divided into 2 groups: diabetic </span></span><em><span style="background:white">training</span></em> <span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.65pt">and diabetic control; A healthy control group was also considered. The training group practiced HIIT 4 days a week for 8 weeks. Data were analyzed using SPSS software version 23 and one-way ANOVA and Tukey post hoc tests.</span></span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 319.15pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results: </span></b><span new="" roman="" style="font-family:" times="">MAFbx protein content showed a significant decrease after 8 weeks of HIIT (P=0.0001); Tukey post hoc test showed that this change was significant between pairs groups of diabetic training and diabetic control and also between pairs groups of diabetic control and healthy control (P=0.0001). MuRF1 protein content showed a significant decrease (P=0.0001); This was a significant difference between the pairs groups of diabetic training and diabetic control, diabetic training and healthy control groups, as well as diabetic control and healthy control groups (P=0.0001).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 319.15pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion: </span></b><span new="" roman="" style="font-family:" times="">HIIT seems to can inhibit the process of atrophy and autophagy of cardiomyocytes by reducing the content of MAFbx and MuRF1 proteins in the hearts of diabetic subjects.</span></span></span></span></span> تمرین تناوبی پرشدت, بطن چپ, پروتئین آتروفی عضله F-Box, پروتئین عضله انگشت حلقه‌ی-1, دیابت نوع دو High-Intensity Interval Training, Left Ventricle, Muscle Atrophy F-box Protein, Muscle Ring-Finger Protein 1, Type 2 Diabete 175 184 http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1808-1&slc_lang=fa&sid=1 Abdol Nasser Seidi عبدالناصر سیدی 0000-0002-3237-3106 No Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran گروه تربیت بدنی و علوم ورزشی، واحد علی‌آباد کتول، دانشگاه آزاد اسلامی، علی‌آباد کتول، ایران Neda Aghaei Bahmanbeglou ندا آقایی بهمن‌بگلو nedaghaei@gmail.com : 0000-0003-0972-1288 Yes Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran گروه تربیت بدنی و علوم ورزشی، واحد علی‌آباد کتول، دانشگاه آزاد اسلامی، علی‌آباد کتول، ایران Habib Asgharpour حبیب اصغرپور 0000-0002-4116-981X No Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran گروه تربیت بدنی و علوم ورزشی، واحد علی‌آباد کتول، دانشگاه آزاد اسلامی، علی‌آباد کتول، ایران Mozhgan Ahmadi مژگان احمدی 0000-0002-2431-6953 No Department of Physical Education and Sport Science, Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran گروه تربیت بدنی و علوم ورزشی، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران