Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
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000
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fa
jalali
1401
12
1
gregorian
2023
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22
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online
1
fulltext
fa
تأثیر تجویز خوراکی اینترلوکین 2 نوترکیب انسانی بر نشانگرهای مولکولی نوروپاتی دیابتی در مغز مگس سرکه
The Effect of Oral Administration of Recombinant Human Interleukin-2 on Molecular Markers of Diabetic Neuropathy in the Brain of Drosophila Melanogaster
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">مقدمه:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> در این مطالعه، اثر تجویز خوراکی اینترلوکین-2 نوترکیب انسانی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">rhIL-2</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">) بر سطح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">NO</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> و فعالیت </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">AChE</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> مغزی در شرایط هیپرگلایسمی القا شده توسط رژیم غذایی با ساکارز بالا (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">HSD</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">)، بعنوان مدل دیابت نوع دو، در مگس سرکه بررسی شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">روشها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt">در این تحقیق تجربی، مگسهای سرکه بالغ از هر دو جنس (30 عدد در هر گروه) به شش گروه دریافت کنندهی غذای استاندارد (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">ND</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt">)، غذایی با ساکارز بالا (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">HSD</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt">)، دریافت کنندهی </span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">rh</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">IL-2</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt">در غلظتهای 01/0 و 1/0 نانوگرم در میلیلیتر بهعلاوه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">ND</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt"> و دریافت کنندهی </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">rhIL-2</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt">در غلظتهای 01/0 و 1/0 نانوگرم در میلیلیتر بهعلاوه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">HSD</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt"> تقسیمبندی شدند. مگسها بهمدت سه هفته روی این محیطهای کشت پرورش داده شدند. مغز مگسهای سرکه استخراج، هموژن و سطح گلوکز، </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">NO</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt"> و فعالیت </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">AChE</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt"> در انتهای آزمایش</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt">ها سنجیده شد.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">یافتهها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> سطح گلوکز، فعالیت کولین استراز و سطح نیتریک اکساید در هموژنای مغز مگسهای سرکه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">HFD</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> در مقایسه با گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">ND</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> افزایش یافت. وزن بدن مگسهای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">HSD</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> در مقایسه با </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">ND</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> کاهش یافت. تجویز </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">rhIL-2</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> در هر دو دوز همراه با </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">HSD</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> بهطور قابل توجهی از بروز این تغییرات پیشگیری کرد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">نتیجهگیری:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">این یافتهها نشان میدهد که </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">rhIL-2</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> تا حدی از نوروپاتی دیابتی در مگسهای سرکه جلوگیری میکند. بهنظر میرسد که اثرات پیشگیرانه این ترکیب از طریق سازِکارهای وابسته به نیتریک اکساید و استیل کولین استراز در مغز واسطهگری میشود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:95%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><span lang="FA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Background:</span></b><span style="font-family:"Times New Roman",serif"> In this study, the effect of oral administration of recombinant human interleukin-2 (rhIL-2) on brain NO level and AChE activity in hyperglycemic conditions induced by high-sucrose diet (HSD), as a type-2 diabetes model, was investigated in Drosophila melanogaster.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Methods:</span></b><span style="font-family:"Times New Roman",serif"> In this experimental research, adult fruit flies of both sexes (30 in each group) were divided into the six groups: receiving normal diet (ND); high-sucrose diet (HSD); ND with rhIL-2 at 0.01 and 0.1 ng/ml; and HSD with rhIL-2 at 0.01 and 0.1 ng/ml. Flies were bred on these culture media for three weeks. At the end of the experiments, the brains of the flies were extracted, homogenized, and glucose, NO, and AChE activity levels were measured by the kit.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Results:</span></b><span style="font-family:"Times New Roman",serif"> Glucose level, AChE activity and NO level increased in brain homogenate of HFD flies compared to ND group. The body weight of HSD flies was reduced compared to the ND. Administration of rhIL-2 along with HFD significantly prevented these changes.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Conclusion:</span></b><span style="font-family:"Times New Roman",serif"> These findings suggest that rhIL-2 partially prevents diabetic neuropathy in Drosophila. It seems that the preventive effects of this compound are mediated through mechanisms dependent on nitric oxide and acetylcholinesterase in the brain.</span></span></span></span></span><br>
اینترلوکین-2 نوترکیب انسانی, نوروپاتی دیابتی, مگس سرکه, نیتریک اکساید, استیل کولین استراز
Recombinant Human Interleukin-2, Diabetic Neuropathy, Drosophila Melanogaster, Nitric Oxide, Acetylcholinesterase
399
408
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1824-1&slc_lang=fa&sid=1
Maedeh
Emamjomeh
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امام جمعه