Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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admin
2345-4008
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fa
jalali
1402
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gregorian
2024
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fulltext
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اثر تمرینهای تناوبی بر بیان PEPCK در سلولهای کبدی، گلوکز و انسولین در رتهای چاق دیابتی نوع دو
The Effect of Interval Training on PEPCK Expression in Hepatic Tissue, Glucose and Insulin of Obese Rats with Type 2 Diabetes
تخصصي
Special
كاربردي
Applicable
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">مقدمه:</span></span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">با توجه به اهمیت ورزش در پیشگیری و درمان بیماریهای مرتبط با چاقی، مطالعۀ تجربی حاضر با هدف تعیین اثر 6 هفته تمرین تناوبی شدید بر بیان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">PEPCK</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> در بافت کبد همچنین گلوکز و انسولین در رتهای چاق دیابتی نوع دو و مقایسه با گروه چاق غیر دیابتی انجام گرفت.</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">روشها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> 28 سر رت نر ویستار 10 هفتهای (20 </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif"">±</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> 220 گرم) بهواسطۀ 6 هفته رژیم غذایی پُرچرب چاق شدند. سپس 14 رت توسط تزریق درون صفاقی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">mg/Kg</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> 30</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">STZ</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> دیابتی نوع دو شدند. رتهای مورد مطالعه در 4 گروه: 1) چاق (7 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">n</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)، کنترل، 2) چاق تناوبی، 3) دیابتی کنترل، 4) دیابتی تناوبی تقسیم شدند. گروههای تناوبی برای مدت 6 هفته در تمرینهای تناوبی به تعداد 5 جلسه در هفته در قالب 10 تکرار دویدن 40 ثانیهای روی تریدمیل با فواصل استراحتی 2 دقیقهای بین تکرارها شرکت نمودند. در نهایت 48 ساعت پس از آخرین جلسه، سطوح ناشتایی گلوکز، انسولین، بیان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">PEPCK</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> در بافت کبد اندازهگیری و توسط آنوای دو سویه مقایسه شدند.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">یافتهها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> در مقایسه با گروه</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های کنترل، تمرینهای تناوبی در رت</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های دیابتی و غیر دیابتی به کاهش معنی</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">دار گلوکز ناشتا منجر شد (001/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P=</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">). تمرینهای تناوبی همچنین به افزایش انسولین سرم نسبت به گروه کنترل دیابتی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">(006/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P=</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) وکاهش در بیان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">PEPCK</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> نسبت به گروه کنترل دیابتی منجر شد (005/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P=</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">).</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتیجهگیری:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">بهبود گلوکز در پاسخ به تمرینهای تناوبی در رتهای دیابتی نوع دو احتمالاً ریشه در افزایش انسولین و افزایش در بیان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">PEPCK</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> کبدی دارد. اندازهگیری فعالیت یا بیان دیگر آنزیمهای کبدی جهت نتیجهگیری کلی پیشنهاد میشود.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Background:</span></b><span style="font-family:"Times New Roman","serif""> Considering the importance of exercise in the prevention and treatment of diseases related to obesity the objective of this experimental study was to determine the effect of six weeks of intensive interval training on PEPCK expression in liver tissue, glucose and insulin and insulin resistance in obese rats with type 2 diabetes and compared with non-diabetic obese group.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Methods:</span></b> <span style="font-family:"Times New Roman","serif"">28 male Wistar rats aged 10 weeks (220 ± 20 g) were obesed by six weeks of high-fat diet (HFD). Then type 2 diabetes was induced in 14 rats by intraperitoneal injection of STZ (30 mg/kg). Finally, the studied rats were divided into 4 groups (n= 7): 1) control obese, 2) interval obese, 3) control obese diabetic, 4) interval obese diabetic. Interval groups participated in an interval exercise program of five sessions per week for six weeks consists of 10 repetitions of a 40-second run on the treadmill with 2-minute rest (active rest) between repetitions. Finally, 48 hours after the last session, the fasting levels of glucose, insulin, PEPCK expression in liver tissue were measured and compared by two-way ANOVA.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Results:</span></b> <span style="font-family:"Times New Roman","serif"">Compared with control groups, interval training in diabetic and obese rats resulted in significant decrease of fasting glucose (P= 0.001). Interval training also led to an increase in serum insulin compared to the diabetic control group (P= 0.006) and a decrease in PEPCK expression compared to the diabetic control group (P= 0.005).</span><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Conclusion:</span></b> <span style="font-family:"Times New Roman","serif"">Improved glucose response to interval training in type 2 diabetic rats may be rooted in increase insulin with decrease in hepatic PEPCK expression. Measurement of activity or expression of other liver enzymes is suggested for general conclusion.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span></span>
تمرین تناوبی, دیابت نوع دو, مقاومت انسولین, بیان ژنهای گلوکونئوژنیک
Interval training, Type 2 Diabetes, Insulin Resistance, Gluconeogenic Genes Expression
368
377
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1905-1&slc_lang=fa&sid=1
Fatemeh
Nikseresht
فاطمه
نیک سرشت
fateme_nikseresht@yahoo.com