Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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admin
2345-4008
2345-4016
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fa
jalali
1402
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gregorian
2023
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تأثیرتمرین تداومی همراه با مکمل رزوراترول بر بیان ژنهای آتروژین-1 و Foxo1 در کاردیومیوسیت ها در موشهای صحرایی نر دیابتی
The Effect of Combination of Continuous Exercise and Resveratrol Supplementation on Foxo-1 and Atrogin-1 Gene Expression in Cardiomyocytes of Diabetic Male Wistar Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">مقدمه</span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">: </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">بیان فاکتورهای رونویسی </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Foxo</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"> در صورت بروز آتروفی دچار افزایش می</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""><span style="color:black"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">شوند که در حالت دفسفریله، از طریق فعالسازی رونویسی لیگازهای یوبی</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""><span style="color:black"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">کوئیتن مانند آتروژین-1 در تخریب پروتئازومی شرکت می</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""><span style="color:black"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">کنند. بنابراین هدف از انجام پژوهش حاضر بررسی تأثیر هشت هفته تمرین تدوامی با مکمل رزوراترول بر بیان ژن</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""><span style="color:black"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">های آتروژین-1 و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="color:black">Foxo1</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"> بافت بطن چپ قلب در موش</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""><span style="color:black"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">های صحرایی دیابتی است.</span></span></span></span><b><span dir="LTR" lang="X-NONE" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="color:black"></span></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span style="color:black"><b><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Lotus"">روشها: </span></span></b><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">در این پژوهش تجربی 25 سر موش صحرایی نر ویستار 8 هفته و وزن 180-250 گرم بهطور تصادفی به 5 گروه شامل: کنترل (5 سر)، دیابت (5 سر)، دیابت-رزوراترول (5 سر)، دیابت-تمرین هوازی (5 سر)، دیابت-رزوراترول-تمرین هوازی (5 سر) تقسیم شدند. پس از القای دیابت از طریق </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">STZ</span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">حیوانات گروه</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">های دیابتی، برنامۀ تمرین شامل 8 هفته تمرین هوازی با شدت 60 تا 75% حداکثر اکسیژن مصرفی اجرا شد. برای تجزیه</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">وتحلیل داده</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">های پژوهش از تحلیل واریانس یکطرفه و آزمون تعقیبی توکی در نرم</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">افزار </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">SPSS</span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">و در سطح آماری (05/0 ></span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">P</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">) استفاده گردید. </span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span style="color:black"><b><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">یافتهها: </span></span></b><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">نتایج نشان داد القای دیابت با </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">STZ</span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">همراه با رژیم غذایی پُرچرب باعث تفاوت معنادار در میزان بیان ژن آتروژین-1 بین دو گروه </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">DM</span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">با گروه </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">ARDM</span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">شد (02/0 =</span></span><span dir="LTR" lang="X-NONE" style="line-height:92%">P</span><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">)، درحالیکه سطح تغییرات بیان ژنی </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">Foxo1</span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">بین گروه </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">ARDM</span></span> <span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">با دیگر گروه</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">ها معنادار بود (001/0= </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">P</span></span><span lang="FA" style="line-height:92%"><span style="font-family:"B Lotus"">).</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتیجهگیری: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">بهنظر می</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">رسد تمرین هوازی تداومی با شدت متوسط با تغییر در بیان ژنی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">Foxo1</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> به همراه کاهش معنادار بیان ژن آتروژین-1می</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تواند بهعنوان یک روش غیردارویی در فرایند درمان بیماران کاردیومایوپاتی دیابتی مؤثر باشد.</span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></b></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Background:</span></b><b> </b><span style="font-family:"Times New Roman","serif"">Expression of FoxO transcription factors whould increase during certain forms of atrophy. In a dephosphorylated state, FoxOs participate in ubiquitin-mediated proteasomal degradation through the <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/transcription-initiation" title="Learn more about transcriptional activation from ScienceDirect's AI-generated Topic Pages"><span style="text-decoration:none"><span style="text-underline:none">transcriptional activation</span></span></a> of E3-ubiquitin ligases such as MAFbx/atrogin-1.<b> </b>Therefore, the study aimed to determine the effect of combination of continuous exercise and resveratrol supplementation on foxo-1 and Atrogin-1 gene expression in the left ventricular tissue of male Wistar rats</span><b><span style="font-family:"TimesNewRomanPSMT","serif"">.</span></b></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Methods: </span></b><span style="font-family:"Times New Roman","serif"">In this study, 25 male Wistar rats with 180-250 g weight were randomly classified into 5 groups, including healthy control (n=5), diabetic control (n=5), diabetic resveratrol (n=5), diabetic-continuous exercise (n=5), and resveratrol+ continuous exercise+ diabetes (n=5). After inducing diabetes</span> <span style="font-family:"Times New Roman","serif"">by intraperitoneal injection of streptozotocin,</span><span style="font-family:"Times New Roman","serif""><span style="color:black"> animals in expremental groups were</span></span> <span style="font-family:"Times New Roman","serif""><span style="color:black">carried out an 8-week exercise program on a treadmill with 60-75% Vo2max. One-way ANOVA and Tukey test with statistical level (P<0.05) was used to compare the differences between groups.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif""><span style="color:black">Results</span></span></b><b><i><span style="font-family:"Times New Roman","serif""><span style="color:black">:</span></span></i></b><span style="font-family:"Times New Roman","serif""><span style="color:black"> The results showed that gene expression of Atrodin-1 were significantly markedly in the ARDM group compared to the DM group (P= 0.02) but the gene expression of<b> </b>foxo-1 only was significantly changed (P= 0.001) in ARDM group to compared with the other groups (P>0.05). </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,"sans-serif""><b><span lang="EN" style="font-family:"Times New Roman","serif"">Conclusion<i>:</i></span></b><span lang="EN" style="font-family:"Times New Roman","serif""> It seems that the Foxo1 gene expression fluctuations along with the significant decrease in the expression of the atherogen-1 gene can be improving the diabetic heart as a non-pharmacological method.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span></span>
تمرین تداومی, آتروژین-1, پروتئین جعبهی سرچنگالی O -1, بطن چپ, دیابت
Continues Exercise, Type 2 diabetes, FOXO1, Atrogin-1, Cardiac Tissue
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1917-1&slc_lang=fa&sid=1
Reza
Salboukhi
رضا
صلبوخی