Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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admin
2345-4008
2345-4016
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fa
jalali
1402
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gregorian
2023
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تأثیر هشت هفته تمرین استقامتی بر سطوح پروتئینهای آپوپتوزی سیتوکروم C، کاسپاز 8 و کاسپاز 3 در بافت کلیۀ موشهای صحرایی نر دیابتی همراه با سندرم ترک مرفین
The Effect of Eight Weeks of Endurance Training on Levels of Apoptotic Proteins of Cytochrome C, Caspase 8 and Caspase 3 in Kidney Tissue of Diabetic Male Rats with Morphine Withdrawal Syndrome
عمومى
General
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">مقدمه: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">اثرات ورزش بر بافت کلیه در سندرم ترک، مبهم و نامشخص است. در این مطالعه، اثر 8 هفته تمرین استقامتی بر سطوح پروتئین سیتوکروم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">C</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">، کاسپاز 8 و کاسپاز 3 در بافت کلیۀ موشهای صحرایی دیابتی و دیابتی همراه با سندرم ترک مورد بررسی قرار گرفت.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">روش ها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> از 32 سر موش صحرایی نر نژاد ویستار در این مطالعۀ تجربی استفاده شد. بعد از القاء دیابت موشهای صحرایی بهصورت تصادفی به 4 گروه 8 تایی تقسیم شدند. شامل: کنترل دیابت (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)، دیابت مرفین (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D.M</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)، دیابت+تمرین استقامتی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D.ET</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)، دیابت مرفین+تمرین استقامتی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D.M.ET</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">). سپس در گروههای معتاد، جهت وابستگی به مرفین از روش خوراکی استفاده شد. گروههای تمرین 8 هفته پروتکل تمرین استقامتی را اجرا نمودند. در پایان مطالعه هم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">G</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> موشهای صحرایی بیهوش، تشریح و کلیۀ آنها جدا و در </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">دمای 70- درجه جهت بررسیهای بعدی نگه</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">داری شدند. سطوح پروتئین شاخصهای این مطالعه توسط کیتهای الایزا اندازهگیری شدند. دادهها با استفاده از آزمون آنوای یک راهه و تعقیبی توکی در سطح معنیداری 05/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">≤</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> تجزیهوتحلیل شدند.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">یافته</span></span></span></b><b><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""></span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">ها: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتایج این مطالعه کاهش معنیدار سطوح پروتئین کاسپاز 3 در گروههای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D.ET, (P=0.000)D.M.ET</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">(</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P=0.000</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) و سیتوکروم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">C</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> در گروههای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D.ET, (P=0.000) D.M.ET</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P=0.000</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) در مقایسه با گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> را نشان داد</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">(05/0</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">≥</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">). همچنین سطوح پروتئین کاسپاز 8 نیز در گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D.M.ET</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P= 0.015</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) در مقایسه با گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> و گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D.M.ET</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">(</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P= 0.038</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) در مقایسه با گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D.M</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> کاهش معنیداری را نشان داد (05/0</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">≥</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">).<span style="background:yellow"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتیجه</span></span></span></b><b><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">گیری: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تمرین استقامتی </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">احتمالاً میتواند </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">بر میزان کاهش آپوپتوز بافت کلیۀ موشهای صحرایی دیابتی و دیابتی سندرم ترک اثر مثبت و محافظتی داشته باشد.<span style="background:lime"></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Background:</span></b><span style="font-family:"Times New Roman","serif""> The effects of exercise on kidney tissue in withdrawal syndrome are unclear. In this study, the effect of 8 weeks of endurance training on the protein levels of cytochrome C, caspase 8 and caspase 3 in the kidney tissue of diabetic and diabetic rats with withdrawal syndrome was investigated.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Methods:</span></b><span style="font-family:"Times New Roman","serif""> 32 male Wistar rats were used in this experimental study. After the induction of diabetes, the rats were randomly divided into 4 groups of 8. Including: diabetes control (D), morphine diabetes (D.M), diabetes + endurance training (D.ET), morphine diabetes+endurance training (D.M.ET). Then, in addicted groups, the oral method was used for morphine dependence. The training groups performed 8 weeks of endurance training protocol. At the end of the study, all rats were anesthetized, dissected and their kidneys were separated and kept at -70 degrees for further investigations. Protein levels of the indicators of this study were measured by ELISA kits. Data were analyzed using one-way ANOVA and Tukey's post hoc test at a significance level of P≤0.05.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Results:</span></b><span style="font-family:"Times New Roman","serif""> The results of this study showed a significant decrease in caspase 3 protein levels in D.ET (P=0.000), D.M.ET (P=0.000) and cytochrome C levels in D.ET (P=0.000), D.M.ET (P=0.000) groups in Comparison with group D. Also, caspase 8 protein levels in D.M.ET group (P=0.015) compared to D group and D.M.ET group (P=0.038) compared to D.M group showed a significant decrease (P≥0.05).</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Conclusion:</span></b><span style="font-family:"Times New Roman","serif""> Endurance training can probably have a positive and protective effect on the reduction of kidney tissue apoptosis in diabetic and diabetic withdrawal syndrome rats.</span></span></span></span>
تمرین استقامتی, آپوپتوز, بافت کلیه, دیابت, سندرم ترک مرفین
Endurance Training, Apoptosis, Kidney Tissue, Diabetes, Morphine Withdrawal Syndrome
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-114-3&slc_lang=fa&sid=1
Behzad
Azadbakht
بهزاد
آزادبخت