Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
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000
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fa
jalali
1402
2
1
gregorian
2023
5
1
23
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online
1
fulltext
fa
تأثیر چهار هفته تمرین تناوبی شدید همراه با مکمل یاری کورکومین بر بیان ژنهای IRS-I و IRE-1α در بطن چپ موشهای صحرایی مبتلا به دیابت نوع دو
The Effect of Four Weeks of High Intensity Interval Training with Curcumin Supplement on the Expression of IRS-1 and IRE-1α Genes in the Left Ventricle of Type 2 Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:95%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">مقدمه:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> نقص در عملکرد انسولین با تغییرات اگزوژنی عامل مهم ایجاد اختلال در بافت قلب است. هدف از </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">پزوهش حاضر بررسی تاثیر</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">4 هفته تمرین تناوبی شدید همراه با مکمل یاری کورکومین بر ژن</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">IRS-I</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">IRE-1α</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> در بطن چپ موش</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">های صحرایی مبتلا به دیابت نوع دو بود.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:95%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">روشها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> در مطالعهی تجربی حاضر 30 سر موش نر مبتلا شده به دیابت به پنج گروه 6 تایی تقسیم شدند؛ تمرین تناوبی شدید (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">HIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">)، تمرین تناوبی شدید+ کورکومین (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">S+HIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">)، کنترل دیابتی+ کورکومین (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">S+DC</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">)، کنترل دیابتی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">DC</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">)، کنترل سالم (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">NC</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">). پروتکل تمرین تناوبی شدید، پنج روز در هفته و بهمدت چهار هفته اجرا شد. کورکومین با دوز100 میلی</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">گرم به ازای هر کیلوگرم وزن بدن روزانه گاواژ شد. بیان ژن</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">IRS-1</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">IRE-1α</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> با روش </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">qReal time-PCR</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">و تجزیهوتحلیل دادهها با آزمون تحلیل واریانس یکطرفه در سطح آلفای 05/0 انجام شد.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">یافته</span></span></span></b><b><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times=""></span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">ها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> بیان ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">IRS-1</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> در گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">S+HIIT</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> نسبت به گروه</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">DC (003/0P=)</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">S+DC (001/0P=)</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">و در گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">HIIT</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> نسبت به گروه</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">DC (002/0P=)</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">S+DC (019/0P=)</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">کاهش معناداری داشت. ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">IRE-1α</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">در گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">S+HIIT</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> نسبت به </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">گروههای</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">DC</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">(003/0P=)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">S+DC (001/0P=)</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">HIIT (008/0P=)</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">کاهش معناداری داشت.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">نتیجهگیری:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">به نظر می رسد تمرین تناوبی شدید همراه با مکمل یاری کورکومین با بهبود شرایط متابولیکی و عوامل ژنی می تواند روند آپوپتوز میوکارد در بیماران دیابتی را کاهش دهد.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><span new="" roman="" style="font-family:" times="">Impaired insulin function with exogenous changes is a major cause of heart failure. The purpose of this study was to investigate the effect of four weeks of high intensity interval training with curcumin supplement on the expression of IRS-1 and IRE-1α gene in the Left ventricle of type 2 diabetic rats.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In the present experimental study, 30 male mice with diabetes were divided into five groups of 6; high intensity interval training (HIIT), High intensity interval Training+Curcumin(S+HIIT), Diabetic control+curcumin(S+DC), Diabetic Control (DC), Normal control (NC). The High intensity interval training protocol was performed five days a week for four weeks. Curcumin gavage at a dose of 100 mg/kg was performed daily. The expression of IRS-1 and IRE-1α were measured by the qReal-TimePCR method, and data were analyzed by one-way ANOVA at the alpha level of 0.05.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><span new="" roman="" style="font-family:" times=""> IRS-1 gene expression in the S+HIIT group compared to the DC (P=0.003) and S+DC (P=0.001) groups and in the HIIT group compared to the DC (P=0.002), and S groups +DC had a significant decrease (P=0.019). IRE-1α gene was significantly decreased in S+HIIT group compared to DC (P=0.003), S+DC (P=0.001) and HIIT (P=0.008) groups.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><span new="" roman="" style="font-family:" times=""> It seems that HIIT with curcumin supplement can reduce the process of myocardial apoptosis in diabetic patients by improving metabolic conditions and genetic factors.</span></span></span></span>
آپوپتوز میوسیت, تمرین تناوبی شدید, کاردیومیوپاتی دیابتی, مکمل کورکومین
Myocrdial Apoptosis, Cardiomyopathy, Curcumin Supplement, High Intensity Interval Training
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1536-5&slc_lang=fa&sid=1
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