Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
000
000
000
000
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fa
jalali
1402
2
1
gregorian
2023
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23
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online
1
fulltext
fa
تأثیر شش هفته تمرین استقامتی بر برخی از miRهای مرتبط با کاردیومیوپاتی دیابتی، FBS و شاخص مقاومت به انسولین رتهای نر نژاد ویستار
The Effect of Six Weeks of Endurance Training on Some Mirs Related to Diabetic Cardiomyopathy, FBS and Insulin Resistance Index in Male Wistar Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">مقدمه:</span></span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> عامل اصلی مرگ دیابتیها، بیماریهای قلبی است. تمرین ورزشی سبب بهبود مقاومت به انسولین و کاهش مرگومیر بیماران دیابتی میگردد. هدف این تحقیق تأثیر شش هفته تمرین استقامتی بر برخی از </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">miR</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">های مرتبط با کاردیومیوپاتی دیابتی، </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">FBS</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> و شاخص مقاومت به انسولین رتهای نر نژاد ویستار بود.</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">روشها:</span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> رتهای نر نژاد ویستار با میانگین وزن 200</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">گرم و سن 8 هفته، پس از القاء دیابت بهطور تصادفی در چهار گروه شم، کنترل، تمرین استقامتی و سالم طبقهبندی شدند. تمرین استقامتی بهمدت 6 هفته اجرا شد. نحوۀ بیان ژن با استفاده از </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">PCR-Real Time</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> بررسی شد. آزمونهای آماری این پژوهش </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">One Way Anova</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">T</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> همبسته بود. دادهها با روش </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">GapDh</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> و </span></span></span></span><sup><span lang="AR-SA" style="font-family:"B Nazanin"">(</span></sup><sup><span dir="LTR" style="font-family:"Times New Roman","serif"">∆∆</span></sup><sup><span dir="LTR" style="font-family:"Times New Roman","serif"">CT</span></sup><sup><span lang="AR-SA" style="font-family:"B Nazanin"">)- </span></sup><span lang="AR-SA" style="font-family:"B Nazanin"">2 </span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">نرمال شدند.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">یافتهها:</span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> تمرین استقامتی منجر به کاهش معنادار </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">FBS</span></span></span></span> <span lang="AR-SA" style="font-family:"B Nazanin"">(001/0 =</span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="AR-SA" style="font-family:"B Nazanin"">) </span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">و شاخص مقاومت به انسولین </span></span></span></span><span lang="AR-SA" style="font-family:"B Nazanin"">(001/0 =</span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="AR-SA" style="font-family:"B Nazanin"">) </span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">و افزایش معنادار </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">VO2peak </span></span></span></span><span lang="AR-SA" style="font-family:"B Nazanin"">(001/0 =</span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="AR-SA" style="font-family:"B Nazanin"">)</span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> شد. سطح بیان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">miR–373</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">miR-195</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> گروه تمرین در مقایسه با گروه کنترل دیابتی، تغییرات معناداری نداشت </span></span></span></span><span lang="AR-SA" style="font-family:"B Nazanin"">(05/0 =</span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="AR-SA" style="font-family:"B Nazanin"">).</span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> اما اثر تمرین منجر به افزایش بیان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">miR-373 </span></span></span></span> <span lang="AR-SA" style="font-family:"B Nazanin"">(4/0 =</span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="AR-SA" style="font-family:"B Nazanin"">) </span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">و کاهش بیان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">miR-195</span></span></span></span> <span lang="AR-SA" style="font-family:"B Nazanin"">(09/0 =</span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="AR-SA" style="font-family:"B Nazanin"">) </span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">شده بود.<i></i></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">نتیجهگیری: </span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">تمرین هوازی توان هوازی گروه تمرین را بهبود بخشید. ضمناً تمرین منجر به بهبود نسبی عوارض دیابت (افزایش سطح بیان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">miR-373</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> و کاهش سطح بیان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">miR-195</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">) شد. بنابراین توصیه میشود بیماران مبتلا به کاردیومیوپاتی، به سمت تمرین</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="letter-spacing:-.2pt"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">های هوازی گرایش پیدا کنند.</span></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="tab-stops:252.9pt"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Background:</span></b><span style="font-family:"Times New Roman","serif""> <span style="background:white">The main cause of death of diabetic patients is heart disease. Exercise improves insulin resistance and reduces the mortality of diabetic patients. The aim of this research was the effect of six weeks endurance training on some miRs related to diabetic cardiomyopathy, FBS and insulin resistance index in male Wistar rats.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:.65pt">Methods:</span></span></b> <span style="font-family:"Times New Roman","serif"">After induction of diabetes, male Wistar rats with an average weight of 200gr and age of 8 weeks were randomly classified into four groups: sham, control, endurance training and healthy. Endurance training was performed for 6 weeks. Gene expression was investigated using Real Time PCR. The statistical tests of this research were One Way Anova and T Correlated<span style="letter-spacing:-.2pt">. The data were normalized by GapDh method and 2<sup>-(∆∆CT)</sup>.</span></span><sup><span style="font-size:12.0pt"><span style="letter-spacing:-.2pt"></span></span></sup></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Results:</span></b><span style="font-family:"Times New Roman","serif""> <span style="letter-spacing:-.2pt">Endurance training leads to a significant decrease in FBS (P: 0.00) and insulin resistance index (P: 0.00) and</span> there was a significant increase in VO2peak (P: 0.001). The expression levels of miR-373 and miR-195 in the exercise group did not change significantly compared to the diabetic control group (P > 0.05). But the effect of training led to an increase in the expression of miR-373 (P: 0.4) and a decrease in the expression of miR-195 (P: 0.9).</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Conclusion:</span></b><span style="font-family:"Times New Roman","serif""> Aerobic exercise improved the aerobic capacity of the exercise group. In addition, exercise led to the relative improvement of diabetes complications (increasing the expression level of miR-373 and decreasing the expression level of miR-195). Therefore, it is recommended that patients with cardiomyopathy should focus on aerobic exercises.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><span style="font-family:"Times New Roman","serif""></span></span></span></span></span>
ورزش, تمرین استقامتی, کاردیومیوپاتی دیابتی, میکرو RNAها
Exercise, Endurance Training, Diabetic Cardiomyopathies, MicroRNAs
125
136
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1925-1&slc_lang=fa&sid=1
Majid
Kashef
مجید
کاشف