Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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2345-4008
2345-4016
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fa
jalali
1402
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gregorian
2023
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تأثیر پُلیمورفیسم rs61330082 ژن ویسفاتین بر سطح سرمی ویسفاتین، دیابت نوع دو و مقاومت به انسولین در یک جمعیت ایرانی
The Effect of Visfatin Rs61330082 Gene Polymorphism on Serum Level of Visfatin, Type 2 Diabetes and Insulin Resistance in an Iranian Population
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">مقدمه:</span></span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> دیابت یک بیماری متابولیکی و شایعترین اختلال غدد درونریز است. ویسفاتین یک سیتوکین با اثرات شبه انسولینی است که با دیابت ارتباط دارد. پلیمورفیسم </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">rs61330082</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> در پروموتر ژن ویسفاتین قرار دارد و بیان ژن ویسفاتین را تنظیم میکند. هدف این مطالعه، بررسی ارتباط پلیمورفیسم </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">rs61330082</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> ژن ویسفاتین با متغیرهای بیوشیمیایی و تنسنجی، سطح ویسفاتین، بیماری دیابت و مقاومت به انسولین است.</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">روشها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> این مطالعه مورد-شاهدی بر روی 80 فرد مبتلا به دیابت و 80 کنترل (افراد بدون دیابت) انجام شد. پارامترهای بیوشیمیایی و آنتروپومتریک با روش استاندارد تعیین شدند. سطح انسولین و ویسفاتین با تکنیک الایزا اندازهگیری شد. تعیین ژنوتیپ با روش واکنش زنجیرهای پلیمراز- چندشکلی طول قطعه محدود انجام شد. دادهها با نرمافزار </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">SPSS</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> تجزیه</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">وتحلیل شد. </span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">یافتهها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> در حاملین ژنوتیپ</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">های پلیمورفیسم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">rs61330082</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">، سطح لیپو</span></span></span><em><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="font-style:normal">پروتئین</span></span></span></span></span></em><em> </em><em><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="font-style:normal">با</span></span></span></span></span></em><em> </em><em><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="font-style:normal">چگالی </span></span></span></span></span></em><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span b="" lotus="" style="font-family:">پایین </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">(</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">LDL-C</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) در افراد سالم و میزان قند خون ناشتا در افراد بیمار تفاوت معنیدار نشان داد. ارتباطی بین این پلیمورفیسم و ویسفاتین یافت نشد. تحلیل رگرسیونی ژنوتیپها نشان داد ژنوتیپهای پلیمورفیسم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">rs61330082</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> ارتباطی با بیماری دیابت نوع دو و مقاومت به انسولین ندارد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">نتیجهگیری:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> پلیمورفیسم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">rs61330082</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> با </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">LDL-C</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> در افراد سالم و قندخون ناشتا در بیماران ارتباط دارد.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> پلیمورفیسم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">rs61330082</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> ویسفاتین ارتباطی باسطح ویسفاتین و بیماری دیابت نوع دو و مقاومت به انسولین ندارد.</span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Background:</span></b><span new="" roman="" style="font-family:" times=""> Type 2 diabetes is a metabolic disease and the most common endocrine disorder. Visfatin is a cytokine with insulin-mimetic effects which is associated with diabetes. The rs61330082 polymorphism is located in the promoter region of visfatin gene and regulates visfatin gene expression. The aim of this study is to investigate the association of rs61330082 polymorphism of the visfatin gene with biochemical and anthropometric variables, visfatin level, diabetes and insulin resistance.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> This case‑control study was performed on 80 patients with type 2 diabetes and 80 controls (persons without diabetes). Biochemical and anthropometric parameters were determined using standard methods. Insulin and visfatin levels were measured by ELISA method. Genotype was determined by polymerase chain reaction-restriction fragment length polymorphism method. The data was analyzed with SPSS software.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><span new="" roman="" style="font-family:" times=""> LDL-C level in healthy people and fasting blood sugar level in patients showed a significant difference in carriers of rs61330082 polymorphism genotypes. No associations was found between visfatin level and single nucleotide polymorphism rs61330082 in the current study. Regression analysis showed that genotypes of rs61330082 polymorphism are not significantly associated with type 2 diabetes and insulin resistance.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Conclusion: </span></b><span new="" roman="" style="font-family:" times="">The rs61330082 polymorphism is associated with LDL-C in healthy subjects and FBS in patients with T2D. Visfatin rs61330082 polymorphism is not associated with visfatin level and type 2 diabetes and insulin resistance.</span></span></span></span></span>
ویسفاتین, پلیمورفیسم تک نوکلئوتیدی, نیکوتین آمید فسفو ریبوزیل ترانسفراز, مقاومت به انسولین
Visfatin, Single Nucleotide Polymorphism, Nicotinamide Phosphoribosyl Transferase, Insulin Resistance
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1910-1&slc_lang=fa&sid=1
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Soltanzadeh
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