Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
000
000
000
000
000
fa
jalali
1402
8
1
gregorian
2023
11
1
23
4
online
1
fulltext
fa
بررسی اثر عصارۀ قارچ کامبوجا و سیر بر روی پارامترهای بیوشیمیایی خون موشهای صحرایی سالم و دیابتی
The Effect of Kombucha Mushroom and Garlic Extracts on Blood Biochemical Parameters of Healthy and Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">مقدمه: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">بیماری دیابت با تغییرات هورمونی و بیوشیمیایی همراه بوده و با تولید رادیکالهای آزاد، نقش مهمی در پیدایش بسیاری از آسیبهای متابولیسمی ایفا مینماید. گیاه سیر و قارچ کامبوجا بهدلیل خواص آنتیاکسیدانی نقش عمدهای در پیشگری و درمان عوارض ناشی از استرسهای اکسیداتیو اعمال مینمایند. مطالعۀ حاضر با هدف بررسی اثر عصارۀ قارچ کامبوجا و سیر بر روی پارامترهای بیوشیمیایی خون موشهای صحرایی سالم و دیابتی انجام شد.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">روشها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">40 سر موش صحرایی نر نژاد ویستار، به </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۵</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">گروه </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۸</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تایی شامل: گروه کنترل، گروه کنترل منفی( شاهد) (دیابتی، با </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">mg/kg</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">55 استرپتوزوتوسین ب</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">ه</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">صورت درون صفاقی)، گروه تجربی 1 (دیابتی + </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">mg/kg</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۵</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">0 عصارۀ سیر) تجربی </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۲ (</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">دیابتی + </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۵</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">mg/kg</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> قارچ کامبوجا) و گروه تجربی</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۳ (</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">دیابتی + </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">mg/kg</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۵</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">0 قارچ کامبوجا و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">mg/kg</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۵</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">0 عصارۀ سیر) تقسیم شدند. بعد گذشت دو ماه از دیابتی شدن موشها عصاره</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">ها بهصورت زیر جلدی بهمدت 2 هفته تزریق شد. بعد از آن موشها با کتامین و زایلیزین بیهوش شده و خونگیری مستقیم از قلب انجام شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">یافتهها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">گروه </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">کنترل منفی(شاهد)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> نسبت به گروه کنترل دارای گلوکز، تریگلیسرید، کلسترول و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">LDL</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> بالاتر و انسولین و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">HDL</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> پایینتر بود. گروههای درمانی موجب کاهش معنیداری در سطح گلوکز، کلسترل، تری گلیسیرید و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">LDL</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> و افزایش معنیدار </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">HDL</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> و انسولین نسبت به گروه </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">کنترل منفی(شاهد) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">شد (05/0> </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">). بیشترین اثر درمانی را گروه سیر + کامبوجا نشان داد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتیجهگیری:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> عصارۀ ترکیبی کامبوجا و سیر اثرات مطلوبی بر کنترل قند و کاهش کلسترول و </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تری گلیسیرید</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">دارد.</span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Background:</span></b><span style="font-family:"Times New Roman","serif""> <span style="background:white">Diabetes is associated with hormonal and biochemical changes, and with the production of free radicals and oxidative stress, it plays an important role in the occurrence of many metabolic injuries. Garlic plant and kombucha mushroom play a major role in the prevention and treatment of complications caused by oxidative stress due to their antioxidant and anti-apoptotic properties.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:.65pt">Methods:</span></span></b> <span style="font-family:"Times New Roman","serif"">40 male Wistar rats, divided into five groups of eight, including: control (diabetic and receiving citrate buffer), </span>Negative control <span style="font-family:"Times New Roman","serif"">group (diabetic, with 55 mg/kg of streptozotocin intraperitoneally), experimental group 1 (diabetic and receiving 50 mg/kg garlic extract), experimental group 2 (diabetic and receiving 50 mg/kg kombucha mushroom) and experimental group 3 (diabetic and receiving 50 mg/kg kombucha mushroom and 50 mg/kg of garlic extract) were divided. After two months passed after the mice became diabetic, the extracts were injected subcutaneously for two weeks. After that, the rats were anesthetized with ketamine and xylisine, and blood was taken directly from the heart. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Results:</span></b><span style="font-family:"Times New Roman","serif""> The </span>Negative control <span style="font-family:"Times New Roman","serif"">group had higher glucose, triglyceride, cholesterol and LDL and lower insulin and HDL than the control group. The treatment groups of garlic, kombucha and garlic + kombucha caused a significant decrease in glucose, cholesterol, triglyceride and LDL levels and a significant increase in HDL and insulin compared to the </span>Negative control <span style="font-family:"Times New Roman","serif"">group (p < 0.05). The highest therapeutic effect was shown by the garlic + kombuja group. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Conclusion:</span></b><span style="font-family:"Times New Roman","serif""> The combined extract of kombucha and garlic has favorable effects on sugar control and reducing cholesterol and Triglyceride.</span></span></span></span></span><br>
<br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-family:"Times New Roman",serif" lang="EN"></span></b></span></span></span></span>
کامبوجا, سیر, دیابت, گلوکز, کلسترول, تریگلیسیرید
Kombucha, Garlic, Diabetes, Glucose, Cholestrol, Triglyceride
214
224
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-133-8&slc_lang=fa&sid=1
Soleiman
Ghamaridaz
سلیمان
سلیمان قمری داز