Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
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fa
jalali
1402
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gregorian
2023
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نقش تمرین منتخب شنا به همراه عصارۀ لیکورایز بر مسیرهای سیگنالینگ SIRT1 در عضلۀ نعلی موشهای صحرایی نر دیابتی نوع دو
The Role of Licorice Extract and Selected Swimming Exercise on Sirt1 Signaling Pathways in Soleus Muscle of Diabetic Male Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">مقدمه:</span></span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> سیر توئین-1 بهعنوان سنسور متابولیک کلیدی در بافتهای مختلف از جمله عضلۀ اسکلتی و بافت شناخته شده است که میتواند نقش محوری در تنظیم متابولیسم گلوکز و لیپیدها از طریق فعالیت دی استیلاز ایفا کند. هدف از تحقیق حاضر بررسی نقش عصارۀ لیکورایز و تمرین منتخب شنا بر مسیرهای سیگنالینگ </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">SIRT1</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> در عضلۀ نعلی موشهای صحرایی نر دیابتی بود.</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">روشها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">پژوهش از نوع تجربی و بنیادی و روش کار یک کارآزمایی تجربی با ماهیت توصیفی_ تحلیلی است. 50 سر موش خریداری شد و در 5 گروه 10 تایی تقسیم شدند. برنامۀ تمرینی اصلی شامل شنا کردن در تانکر ویژه جوندگان مخزن آب ویژه شنا بهمدت 6 هفته و </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۵</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">جلسه در هفته بود. بهمنظور دیایتی کردن موشها استرپتوزوتوسین تزریق شد. </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۷</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">روز پس از تزریق قند خون موشها اندازهگیری شد و موشهایی که قند خونشان بالاتر از </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">۲۵۰</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">میلیگرم بر دسیلیتر بود بهعنوان نمونۀ دیابتی انتخاب میشوند. به دو گروه از موشهای دیابتی عصارۀ ریشۀ لیکورایز خورانده شد. جهت تجزیهوتحلیل یافتههای تحقیق از آزمون آنالیز واریانس دو راهه و با استفاده از نرمافزار </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">SPSS26</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> استفاده شد.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">یافتهها:</span></span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتایج نشان داد اثر متقابل تمرین شنا و مصرف عصارۀ لیکورایز بر مسیر سیگنالینگ </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">SIRT1</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> از نظر آماری معنیدار است (002/0 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">).</span></span></span><i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"></span></span></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">نتیجهگیری: </span></span></span></span></b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">SIRT1</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> نقش مهمی در کنترل هموستاز گلوکز بازی میکند. در واقع، تحت شرایط دیابتی فعالیت و میزان بیان ژن </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">SIRT1</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> در بافتهای مختلف علیرغم نقش متابولیکی و مهم، کاهش مییابد. تمرین شنا به همراه مصرف مکمل لیکورایز با تنظیم مثبت بیان ژن </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">SIRT1</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> میتواند در پیشگیری و درمان بیماری دیابت مؤثر باشد.</span></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Background:</span></b><span style="font-family:"Times New Roman","serif""> <span style="background:white">Sirtuin-1 is known as a key metabolic sensor in various tissues, including skeletal muscle and tissue, which can play a pivotal role in regulating glucose and lipid metabolism through diacetylase activity. The aim of the present study was to investigate the role of licorice extract and swimming exercise on SIRT1 signaling pathways in the soleus muscle of diabetes male rats.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:.65pt">Methods:</span></span></b> <span style="font-family:"Times New Roman","serif"">The research is of an experimental and fundamental type, and the working method is an experimental work with a descriptive-solution nature. Fifty mice were bought and divided into 5 groups of 10. The main training program consisted of swimming in the rodent tank of the special swimming water tank for 6 weeks and 5 sessions per week. In order to induce diabetes in the mice, streptozotocin was injected. Seven days after the injection, the blood sugar of the mice was measured, and the mice with blood sugar more than 250 ml/dL were selected as diabetes samples. Licorice root extract was given to two groups of rats. In order to analyze, two-way analysis of variance was used using SPSS26 software.</span><sup><span style="font-size:12.0pt"><span style="letter-spacing:-.2pt"></span></span></sup></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Results:</span></b><span style="font-family:"Times New Roman","serif""> <span style="letter-spacing:-.2pt">The results showed that the interaction effect of swimming training and consumption of licorice extract on SIRT1 signaling pathways is statistically significant (P= 0.002).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Conclusion:</span></b><span style="font-family:"Times New Roman","serif""> SIRT1 plays an important role in the control of glucose homeostasis. In fact, under certain conditions, the activity and expression level of SIRT1 gene decreases in different SI tissues due to its important metabolic role. Swimming training along with licorice supplement may be used for prevention and treatment of diabetes by positive regulation of SIRT1 gene expression.</span></span></span></span></span>
عصارۀ لیکورایز, تمرین شنا, مسیرهای سیگنالینگ SIRT1, عضلۀ نعلی, دیابت
licorice extract, Swimming training, SIRT1 signaling pathways, Soleus muscle, Diabetes
318
326
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1980-1&slc_lang=fa&sid=1
Maryam
Taheri
مریم
طاهری