Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
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000
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fa
jalali
1402
8
1
gregorian
2023
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1
fulltext
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اثر تمرین هوازی بر عملکرد سیستم رنین-آنژیوتانسین موضعی و مقاومت به انسولین در عضله گاستروکنمیوس موشهای دیابتی نوع دو
The Effect of Aerobic Exercise on the Function of Local Renin-Angiotensin System and Insulin Resistance in the Gastrocnemius Muscle of Type 2 Diabetic Mice
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">مقدمه:</span></span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"> هدف مطالعۀ حاضر بررسی اثر تمرین هوازی بر عملکرد سیستم رنین-آنژیوتانسین موضعی و مقاومت به انسولین در عضلۀ دوقلو موش</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""><span style="color:black"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">های دیابتی نوع دو بود.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">روشها:</span></span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تعداد 18 سر موش نر نژاد </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">C57BL/6</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">به دو گروه تقسیم شدند: 1- موش</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های تغذیه شده با رژیم غذایی استاندارد بهمدت 12 هفته (گروه کنترل)، 2- موش</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های تغذیه شده با رژیم غذایی پُرچرب بهمدت 12 هفته همراه با تزریق استرپتوزوتوسین (گروه دیابت). موش</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های دیابتی بهطور تصادفی به دو گروه تقسیم شدند: 1- گروه دیابت-بی تحرک، 2- گروه دیابت-تمرین. موش-های تمرینی بهمدت هشت هفته و پنج روز در هفته با سرعت متوسط 19 متر بر دقیقه و مدت 45 دقیقه بر روی نوارگردان تمرین کردند. 24 ساعت پس از آخرین جلسه تمرینی، موش</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">ها قربانی شدند. عضلۀ دو قلو استخراج و برای اندازه</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">گیری بیان ژن</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های آنژیوتانسین 2، گیرندۀ </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">Mass</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">، گیرنده </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">At1</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> و انتقال دهنده گلوکز 4 (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">Glut4</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) در فریز 80- درجۀ سانتیگراد نگهداری شد.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">یافتهها:</span></span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتایج نشان داد بیان ژن گیرندۀ </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">Mass</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">Glut4</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> در موش</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های دیابت-تمرین بهطور معناداری از گروه دیابت-بی</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تحرک بیشتر بود. همچنین، بیان ژن آنژیوتانسین 2، شاخص مقاومت به انسولین و گلوکز خون ناشتا در عضله موش</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های دیابت-تمرین در مقایسه با موش</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های دیابت-بی</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تحرک به</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">طور معناداری کمتر بود. همچنین، بیان گیرنده </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">At1</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> در گروه دیابت-تمرین نسبت به گروه دیابت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">-بی</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تحرک به طور غیرمعناداری کمتر بود.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">نتیجهگیری:</span></span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">یافته</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های مطالعۀ حاضر نقش مهم تمرین ورزشی در بهبود سیستم رنین-آنژیوتانسین موضعی در عضلۀ اسکلتی را نشان میدهد. هشت هفته تمرین هوازی از طریق کاهش بیان آنژیوتانسین 2، گیرنده </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">At1</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> و افزایش سطوح گیرنده </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">Mass</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">، می</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تواند سبب کاهش مقاومت به انسولین عضلانی و بهبود بیماری دیابت نوع دو </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">شود.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Background:</span></b> <span style="font-family:"Times New Roman","serif"">The purpose of this study was to investigate the impact of aerobic exercise on the function of local renin-angiotensin system and insulin resistance in the Gastrocnemius muscle of type 2 diabetic mice.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Methods:</span></b><span style="font-family:"Times New Roman","serif""> The number of 18 male C57BL/6 mice were divided into two groups: 1. Mice fed with a standard diet for 12 weeks (control group), 2. Mice fed with a high-fat diet for 12 weeks with streptozotocin injection (diabetes group). Diabetic mice were randomly divided into two groups: 1. Diabetes-Sedentary group, 2. Diabetes-exercise group. The mice of the training group exercised on the treadmill for eight weeks and five days/week with an average speed of 19 m/min and for 45 minutes. 24 hours after the last training session, the mice were sacrificed. The Gastrocnemius muscle tissue was extracted and stored in -80°C freezer to measure the expression level of angiotensin 2, Mass receptor, At1 receptor and glucose transporter 4 (Glut 4).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Results:</span></b><span style="font-family:"Times New Roman","serif""> The results showed that the expression of Mass and Glut4 receptor in diabetes-exercise mice was significantly higher than diabetes-sedentary group. Also, the expression of angiotensin 2, HOMA-IR, and fasting glucose in the muscle of diabetes-exercise mice were significantly lower vs. diabetes-sedentary mice. Also, the expression of At1 receptor in the diabetes-exercise group was insignificantly lower than in the diabetes-sedentary group.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:92%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Conclusion:</span></b><span style="font-family:"Times New Roman","serif""> The findings of the present study show the important role of exercise in improving the local renin-angiotensin system in skeletal muscle. Eight weeks of aerobic training can reduce muscular insulin resistance and improve type 2 diabetes by decreasing the expression of angiotensin 2, At1 receptor and increasing the Mass receptor.</span></span></span></span></span></span>
تمرین هوازی, سیستم رنین-آنژیوتانسین, مقاومت به انسولین, عضله دوقلو, دیابت نوع دو
Aerobic Exercise, Renin-Angiotensin System, Insulin Resistance, Gastrocnemius Muscle, Type 2 Diabetes
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1864-3&slc_lang=fa&sid=1
Fatemeh
Sabaghian
فاطمه
صباغیان