Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
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fa
jalali
1402
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gregorian
2024
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fulltext
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تأثیر هشت هفته تمرین هوازی بر بیان ژن MuRF1 در سلولهای عضلۀ قلب موشهای صحرایی نژاد ویستار با دیابت القایی
The Effect of Eight Weeks' Aerobic Exercise Protocol on Murf1 Gene Expression in Cardio-Myocyte of Diabetic Wistar Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><a name="_Hlk142578572"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">مقدمه:</span></span></span></span></b></a> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">کاردیومیوپاتی دیابتی یکی از عوامل خطرزای اصلی برای عوارض قلبی- عروقی دیابت محسوب می</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">گردد که می</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">تواند ناشی از آتروفی سلول</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">های عضلۀ بافت قلب باشد. ژن </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">MuRF1</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> بهعنوان یکی از عوامل اصلی در آتروفی بافت قلب معرفی شده است. این مطالعه به منظور تعیین اثر تمرین هوازی بر بیان ژن </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">MuRF1</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> در سلول</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">های عضلۀ قلب موش</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">های صحرایی نژاد ویستار با دیابت القایی انجام شد.</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">روشها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> در این مطالعۀ تجربی 30 سر موش صحرایی نر نژاد ویستار با سن هشت هفته و میانگین وزنی 288 گرم بهطور تصادفی به سه گروه 10 تایی شامل کنترل سالم، کنترل دیابتی وگروه تمرین هوازی تقسیم شدند. پروتکل تمرینی بهمدت 8 هفته تمرین هوازی تداومی شامل دویدن روی نوارگردان مخصوص جوندگان با شدت 60 تا 75 درصد </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Vo2max</span></span></span><span style="font-size:10.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> و</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> به تعداد 5 روز در هر هفته اجرا شد. بیان ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">MuRF1</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> با استفاده از روش </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Real-Time</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">PCR</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> بررسی گردید.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">یافتهها:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">در ابتدا القاء دیابت موجب افزایش آماری معنی</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">دار گلوکز سرم و بیان ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">MuRF1</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> در گروه کنترل دیابتی و گروه تمرین هوازی شد (05/0> </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، اما پس از هشت هفته تمرین منظم هوازی منجر به کاهش معنادار بیان ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">MuRF1</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> گروه تمرین دیابتی در مقایسه با گروه کنترل دیابتی شد (05/0> </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">).</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">نتیجهگیری:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">بهنظر می</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">رسد تمرین هوازی منظم با شدت متوسط که منجر به کاهش گلوکز خون و نیز کاهش بیان ژنی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">MuRF1</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> گردید احتمالاً روندآتروفی بافت قلبی را نیز کند کرده است. هرچند مطالعات بیشتر برای تأیید این نظریه احساس می</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">گردد.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span sans-serif="" style="font-family:Calibri,"><b><span advp46413a="" style="font-family:">Background: </span></b><span advp46413a="" lang="EN" style="font-family:">Diabetic cardiomyopathy is one of the main risk factors related to diabetes, which can be caused by atrophy of the cardiomocytes.</span><span advp46413a="" style="font-family:"> It is supposed</span><span advp46413a="" lang="EN" style="font-family:"> that the MuRF1 gene intermediate as an agent for heart atrophy.</span><span advp46413a="" style="font-family:"> Hence, the purpose of current study was to investigate the effect of eight weeks' aerobic exercise protocol on murf1 gene expression in cardio-myocyte of diabetic Wistar rats.</span><span lang="FA" dir="RTL" style="font-family:"B Zar""></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span sans-serif="" style="font-family:Calibri,"><b><span advp46413a="" style="font-family:">Methods: </span></b><span advp46413a="" lang="EN" style="font-family:">In this study, 30 male Wistar rats with an age of eight weeks and an average weight of 288 grams were randomly divided into three groups of 10 including healthy control, diabetic control and aerobic exercise group after two weeks of adaptation to the environment.</span><span advp46413a="" style="font-family:"> The exercise groups went under aerobic training programs using treadmill (5 days/wks., for 8 wks.) 60%-75% Vo2max. MuRF1 mRNA level was measured in cardio myocyte using Real-Time PCR. The results were compared by statistical methods. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span sans-serif="" style="font-family:Calibri,"><b><span advp46413a="" style="font-family:">Results:</span></b><span advp46413a="" style="font-family:"> The changes in the expression of Murf1 genes expression in cardio-myocyte of diabetes group were significantly higher than the other groups (P</span><span new="" roman="" style="font-family:" times="">< .05</span><span advp46413a="" style="font-family:">). The expression of Murf1 gene in continuous training group reduced significantly after eight weeks' aerobic exercise training (P󠇟</span><span new="" roman="" style="font-family:" times="">< .05</span><span advp46413a="" style="font-family:">).</span><b> </b></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:92%"><span sans-serif="" style="font-family:Calibri,"><b><span advp46413a="" style="font-family:">Conclusion: </span></b><span advp46413a="" style="font-family:">This study demonstrated that eight weeks' aerobic exercise training can lead to reduction of Murf1 genes expression level and might be a good prescription for diabetic persons. However, further studies are needed to confirm this theory. </span></span></span></span></span>
عضلۀ انگشت حلقهای-1, تمرین ورزش تداومی, سلول عضلۀ قلب, دیابت
Murf1, Continues Training, Cardio myocyte, Diabetes
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1917-2&slc_lang=fa&sid=1
Masoumeh
Azizi
معصومه
عزیزی
scienceinsport@yahoo.com