Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
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000
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000
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fa
jalali
1403
3
1
gregorian
2024
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24
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online
1
fulltext
fa
تأثیر تمرین تناوبی پُرشدت (HIIT) بر میزان پروتئینهای مرتبط با مسیر میتوفاژی (LC3 و BNIP3L) در بافت عضلانی نعلی رتهای مبتلا به دیابت نوع دو
The Effect of High-Intensity Interval Training (HIIT) on the Content of Proteins Related to the Mitophagy Pathway (LC3 And BNIP3L) in the Muscle Tissue Soleus of Rats with Type 2 Diabetes
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">مقدمه:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> میتوفاژی نوعی مرگ سلولی برای تنظیم کیفیت میتوکندری است که در شرایط بیماری مانند دیابت میتواند منجر به اختلال شود؛ بنابراین، هدف از انجام تحقیق حاضر، تأثیر تمرین تناوبی پُرشدت (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">HIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) بر میزان پروتئینهای مرتبط با مسیر میتوفاژی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">LC3</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">BNIP3L</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) در بافت عضلانی نعلی رتهای مبتلا به دیابت نوع دو است.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">روشها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> در این مطالعۀ تجربی، 18 سر رت نر سه ماهه از نژاد اسپراگداولی با میانگین وزن 30</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">270 گرم انتخاب شدند. رتها از طریق تزریق درونصفاقی محلول استرپتوزوتوسین و نیکوتینآمید مبتلا به دیابت نوع دو شدند. این رتها به روش تصادفی به دو گروه، تمرین دیابتی و کنترل دیابتی تقسیم شدند؛ یک گروه کنترل سالم نیز در نظر گرفته شد. گروه تمرینی، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">HIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> را بهمدت هشت هفته با شدتی معادل 85 تا 95 درصد حداکثر سرعت انجام دادند. تجزیهوتحلیل دادهها از طریق آزمون آنوای یکطرفه در نرمافزار گرافپد پریسم نسخۀ 5/9 انجام شد. سطح معناداری </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">05/0</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">≤</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">در نظر گرفته شد.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">یافتهها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">محتوای </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">پروتئینهای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">LC3</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">BNIP3L</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> افزایش معنیداری را پس از</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">هشت هفته </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">HIIT</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> نسبت به هر دو گروه کنترل دیابتی و کنترل سالم نشان داد (0001/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">= </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">).</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">نتیجهگیری:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> میتوان نتیجه گرفت که </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">HIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> با افزایش عوامل مرتبط با میتوفاژی میتواند سبب پاکسازی میتوکندریهای ناکارآمد در عضلۀ آزمودنیهای دیابتی شود؛ با این وجود میتوفاژی بیش از حد نیز میتواند سبب نقص عملکردی در تنظیم کیفیت میتوکندریها شود.</span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Background:</span></span></b><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""> <span style="background:white">Mitophagy is a type of cell death that regulates the quality of mitochondria and can lead to disorders in diseases such as diabetes. Therefore, the purpose of this study was to investigate the effect of high-intensity interval training (HIIT) on the content of proteins related to the mitophagy pathway (LC3 and BNIP3L) in muscle tissue soleus of rats with type 2 diabetes.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.65pt">Methods:</span></span></span></b> <span style="background:white"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">In this experimental study, 18 three-month-old male Sprague Dawley rats with an average weight of 270±30 g were selected. Rats were infected with type 2 diabetes by intraperitoneal injection of a streptozotocin and nicotinamide solution. Rats were randomly divided into two groups: diabetic and diabetic. A healthy control group was also included. The training group performed HIIT for eight weeks at an intensity of 85-95% of the maximum speed. Data analysis was performed using a one-way ANOVA test in GraphPad Prism version 9.5 software. A significance level of P≤ 0.05 was considered statistically significant.</span></span></span><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Results:</span></span></b> <span style="background:white"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">The levels of LC3 and BNIP3L proteins significantly increase after eight weeks of HIIT compared to both the diabetic and healthy control groups (P= 0.0001).</span></span></span><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b> <span style="background:white"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">It can be concluded that HIIT by increasing the factors related to mitophagy can cause the cleaning of dysfunctional mitochondria in the muscle of diabetic subjects; However, excessive mitophagy can also cause functional defects in regulating the quality of mitochondria.</span></span></span></span></span></span></span>
تمرین تناوبی پُرشدت, عضلۀ نعلی, پروتئین LC3, پروتئین BNIP3L, دیابت نوع دو
High-Intensity Interval Training, Soleus Muscle, LC3 Protein, BNIP3L Protein, Type 2 Diabetes
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-190-4&slc_lang=fa&sid=1
Mahya
Sharifi Rayeni
محیا
شریفیراینی