Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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2345-4008
2345-4016
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fa
jalali
1403
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gregorian
2024
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تغییرات سطح تیوردوکسین احیاء شده/ تیوردوکسین اکسید شده و مقادیر گلوتاتیون و ظرفیت آنتی اکسیدانی کل متعاقب هشت هفته تمرین تناوبی شدید با و بدون مصرف مکمل سیترات در رتهای دیابتی
Changes in Trx-Sh/Trx-S2 Levels and Glutathione Values and Total Antioxidant Capacity Following 8 Weeks of High Intensity Interval Training with and Without Sodium Citrate Supplementation in Diabetic Rats
عمومى
General
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">مقدمه:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> هدف از پژوهش حاضر بررسی </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""><span style="color:black">تغییرات سطح </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">TRx-sh/ TRx-s2</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> و مقادیر </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">GSH</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> و ظرفیت آنتی اکسیدانی کل متعاقب 8 هفته تمرین تناوبی شدید با و بدون مصرف مکمل سیترات در رتهای دیابتی </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">روشها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">دراین مطالعۀ نیمهتجربی 50 سر موش نر نژاد ویستار بهطور تصادفی به 5گروه: کنترل سالم، کنترل دیابتی، دیابتی - تمرین، دیابتی- مکمل دهی سیترات سدیم و دیابتی-مکمل دهی سیترات سدیم -تمرین تقسیم شدند. پروتکل تمرین </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">شامل 8 هفته تمرین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">HIIT</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> با شدت ۹۰</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">درصد سرعت بیشینه بود. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">گروه های مکمل و تمرین </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"Arial","sans-serif"">–</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> مکمل، سه ساعت پیش از تمرین، روزانه (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">mg/kg</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> 764) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">مکمل سیترات سدیم را به شکل محلول در آب دریافت کردند.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> ۷۲ ساعت پس از آخرین جلسۀ تمرین خون گیری انجام و </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">متغیرهای تحقیق به روش الایزا بررسی شدند. برای مقایسه گروههای مطالعه از تحلیل واریانس یک راهه و از آزمون تعقیبی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">LSD</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> در نرمافزار </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">SPSS</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> استفاده شد (05/0 </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"Arial","sans-serif"">≥</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">).</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">یافتهها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">سطوح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">TRx-sh/ TRx-s2</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">GSH</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">TAC</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">بین گروه</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">های پنج</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">گانه تحقیق با توجه به سطح معنی داری (001/0 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">) اختلاف معنیداری داشت (001/0 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">). همچنین نتایج نشان داده سطوح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">TRx-sh/ TRx-s2, TAC, GSH</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">در گروه کنترل-دیابتی (001/0 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">)، دیابتی-تمرین (001/0 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">)، دیابتی-مکمل (001/0 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">) و دیابتی-مکمل-تمرین (001/0 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">) در مقایسه با گروه کنترل سالم کاهش معنی</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">داری داشتند. سطح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">TRx-sh/ TRx-s2</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">GSH</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">TAC</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> در گروه دیابتی-مکمل و دیابتی-مکمل-تمرین در مقایسه با گروه کنترل-دیابتی افزایش معنی</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">داری داشت (001/0 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">). </span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">نتیجه</span></span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif""></span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">گیری:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">با توجه به نتایج تحقیق می</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus"">توان احتمال داد تمرینهای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">HIIT</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> همزمان با مکمل یاری سیترات سدیم بر بهبود دیابت مؤثر باشد و احتمالاً این تأثیر بهواسطۀ افزایش </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">TRx-sh/ TRx-s2, TAC, GSH</span></span></span><span style="font-size:12.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""> اعمال میشود.</span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:90%"><span style="font-family:Calibri,"sans-serif""><b><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">Background:</span></span></b><span style="line-height:90%"><span style="font-family:"Times New Roman","serif""> The present study aimed to investigate the changes in TRx-sh/TRx-s2 levels, GSH values, and total antioxidant capacity following 8 weeks of intense interval training with and without sodium citrate supplementation in diabetic rats.</span></span><b><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""></span></span></span></b></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="font-family:Calibri,"sans-serif""><b><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">Methods:</span></span></b><span style="line-height:90%"><span style="font-family:"Times New Roman","serif""> In this semi-experimental and fundamental study, 50 male Wistar rats were randomly divided into 5 groups: healthy control, control control, selective - exercise, consumption-sodium citrate supplementation, and drink-sodium citrate supplementation. The training protocol included 8 weeks of HIIT training with an intensity of 90% of the maximum speed. The supplement and exercise-supplement groups received daily (764 mg/kg) sodium citrate supplement in the form of a solution in water, three hours before the exercise. 72 hours after the last training session, blood sampling was done and the ELISA method analyzed research variables. To compare study groups, one-way analysis of variance and LSD post hoc test were used in SPSS software (P≥ 0.05).</span></span><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="font-family:Calibri,"sans-serif""><b><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">Results:</span></span></b><span style="line-height:90%"><span style="font-family:"Times New Roman","serif""> The results showed that the levels of TRx-sh/TRx-s2, GSH, and TAC were significantly different among the five research groups (P= 0.001). Also, the results showed the levels of TRx-sh/TRx-s2, TAC, and GSH in the control-diabetic group (P= 0.001), diabetic-exercise (P= 0.001), and diabetic-supplement group (P= 0.001). and diabetic-supplement-exercise (P= 0.001) had a significant decrease compared to the healthy control group. The levels of TRx-sh/TRx-s2, GSH, and TAC in the diabetic-supplement and diabetic-supplement-exercise groups were significantly increased compared to the diabetic control group (P= 0.001). According to the difference in means, the increase of TRx-sh/TRx-s2, TAC, and GSH was higher in the diabetic-supplemented group.</span></span><b><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""></span></span></span></b></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="font-family:Calibri,"sans-serif""><b><span style="line-height:90%"><span style="font-family:"Times New Roman","serif"">Conclusion:</span></span></b><span style="line-height:90%"><span style="font-family:"Times New Roman","serif""> According to the results of the research, it can be assumed that training and sodium citrate supplementation are effective in improving diabetes and this effect is probably exerted by increasing TRx-sh/TRx-s2, TAC, GSH.</span></span><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="line-height:90%"><span style="font-family:"B Lotus""></span></span></span></span></span></span>
سیترات سدیم, آنتی اکسیدان تام, گلوتاتیون, تیوردوکسین, دیابتیابت
Sodium Citrate, Total Antioxidant, Glutathione, Thioredoxin, Diabetes
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-2071-1&slc_lang=fa&sid=1
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Kashfi moghadam
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