Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
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000
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fa
jalali
1403
7
1
gregorian
2024
10
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24
4
online
1
fulltext
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تأثیرهشت هفته تمرینهای تناوبی شدید (HIIT) و مصرف مکمل ال- کارنیتین بر بیان پروتئین BMP7 بافت چربی احشایی در موشهای نر دیابتی ویستار
The Effect of Eight Weeks of High Intensity Interval Training (HIIT) and L -Carnitine Consumption on the Expression of BMP7 Protein in Visceral Adipose Tissue in Male Wistar Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">مقدمه</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">: آدیپوژنز فرآیندی پویا است که منجر به فنوتیپ سلولهای چربی بالغ میشود و نقش بهسزایی در چاقی و دیابت ایفا میکند. پژوهش حاضر با هدف بررسی اثر هشت هفته تمرین تناوبی شدید (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">HIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) و مصرف مکمل ال-کارنیتین بر بیان پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">BMP7</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> بافت چربی احشایی در موشهای ویستار نر مدل دیابتی انجام شد.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">روشها</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">: </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">در یک مطالعۀ حیوانی بالینی- تجربی 50 سر موش صحرایی نر ویستار به 5 گروه مساوی کنترل(</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">C</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، دیابتی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">D</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، دیابتی با مکمل (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">D+LC</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، دیابتی با تمرین (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">D+T</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) و دیابتی با تمرین و مکمل (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">D+LC+T</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) تقسیم شدند. پروتکل تمرینی شامل هشت هفته، هفتهای 5 جلسه ( 6 تا 12 وهلۀ 2 دقیقهای با شدت 90 -85% سرعت بیشینه) بود. هفتهای پنج روز </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">mg/Kg</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">30 ال-کارنیتین از طریق آب آشامیدنی داده شد. چربی احشایی استخراج و بیان پروتئین</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">BMP7</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">با روش وسترن بلات مورد سنجش قرار گرفت. تحلیل داده</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">ها با آزمون</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">های تحلیل واریانس یکطرفه و تعقیبی توکی در سطح معناداری</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">(</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P< 0.05</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">انجام شد.</span></span></span><span lang="AR-SA" style="font-size:13.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">یافتهها</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">:</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">نتایج نشان داد که در هر دو گروه تمرین و تمرین+مکمل میزان بیان پروتئین (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">BMP7</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) افزایش معناداری (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P< 0.001</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) داشت.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">نتیجهگیری</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">: باتوجه به نتایج این تحقیق، بهنظر میرسد انجام تمرین</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">های</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> تناوبی با شدت بالا به تنهایی و به همراه مکمل ال-کارنیتین در افزایش میزان بیان پروتئین مرتبط با آدیپوژنز بافت چربی احشایی مؤثر بود. هر چند اظهار نظر صریح در این مورد مستلزم انجام تحقیقات بیشتر در این زمینه است.</span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:92%"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Background:</span></span></b><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""> Adipogenesis is a dynamic process that leads to the phenotype of mature fat cells and plays a significant role in obesity and diabetes. The present study investigates the effect of Eight –Weeks of High-Intensity Interval Training (HIIT) and L -carnitine Consumption on the expression of BMP7 protein in visceral adipose tissue in diabetic rats.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></b><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""> In an experimental clinical-intervention study, 50 male Wistar rats were divided into 5 equal groups of control (C), diabetic (D), supplemental diabetic (D + LC), diabetic with exercise (D + T), supplement and exercise (D + LC + T). The training program consisted of eight weeks, 5 sessions per week (6 to 12 2-minute sessions with an intensity of 85-90% of the maximum speed). The subjects received 30 mg/kg body weight L-carnitine through drinking water. Visceral fat was extracted and the expression level of BMP7 protein was assessed using Western Blotting. Data analysis was performed by one-way ANOVA and Tukey post hoc test at a significant level of (P< 0.05).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""> The results showed that the expression of BMP7, the adipogenesis stimulating protein, in (D + T) and (D + LC + T) groups increased significantly (P< 0.001).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""> According to the results, it seems that HIIT exercise alone and with L-carnitine Consumption is more effective in the expression of protein associated with adipogenesis expression in visceral fat. However, a clear statement requires further research in this area.</span></span></span></span></span></span>
دیابت, آدیپوژنز, ال-کارنیتین, تمرینهای تناوبی شدید (HIIT)
Diabetes, Adipogenesis, L -carnitine, High Intensity Interval Training (HIIT) (HIIT)
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-2077-1&slc_lang=fa&sid=1
Somayeh
Madanipour
سمیه
معدنی پور