Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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2345-4008
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jalali
1403
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2024
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اثر هشت هفته تمرین هوازی و مکمل ویتامین D3 بر غلظت پروتئین GLUT4 و مقاومت به انسولین در عضلۀ اسکلتی موشهای صحرایی دیابتی
The Effect of Eight Weeks Aerobic Training and Vitamin D3 Supplementation on Glut4 Protein Concentration and Insulin Resistance in the Skeletal Muscle of Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">مقدمه</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">: ناقل 4 گلوکز (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">GLUT4</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، مهم</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">ترین انتقال</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">دهندۀ گلوکز در عضلۀ اسکلتی است. اختلال در بیان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">GLUT4</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> نقش مهمی را در اختلالات هومئوستاز گلایسمیک ایفا می</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">کند. هدف پژوهش حاضر بررسی اثر تمرین هوازی و مکمل ویتامین</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">D3</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> بر سطوح پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">GLU</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">T4</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> و مقاومت به انسولین در عضلۀ نعلی موش</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">های صحرایی دیابتی شده با استرپتوزوتوسین و مصرف غذای پُرچرب بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">روشها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> 40 سر موش صحرایی نر نژاد ویستار پس از القای دیابت نوع دو به وسیلۀ رژیم غذایی پُرچرب (6 هفته) و تزریق استرپتوزوتوسین به طور تصادفی به 5 گروه کنترل سالم (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">HC</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، کنترل دیابتی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">DC</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، دیابت+تمرین هوازی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">DAT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، دیابت+ویتامین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">D3</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">(DVD)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> و دیابت+تمرین هوازی+ویتامین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">D3 (DVDAT)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> تقسیم شدند. موش</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">ها به مدت هشت هفته تحت تمرین هوازی و مکمل</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">دهی با ویتامین</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">D3</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> قرار گرفتند. 24 ساعت پس از آخرین جلسۀ تمرین موشها بیهوش شدند و بافت عضلۀ نعلی برای اندازه</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">گیری محتوای پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">GLUT4</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> جدا شد. همچنین سطوح سرمی انسولین، گلوکز و ویتامین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">D3</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> اندازهگیری شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">یافتهها</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">: <span style="letter-spacing:-.1pt">نتایج آزمون تحلیل واریانس یکطرفه نشان داد که سطوح پروتئین </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">GLUT4</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> در گروه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">DC</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> بهطور معنی</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">داری کمتر از گروه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">HC</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> بود (001/0 ></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">). اما در گروه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">DVDAT</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> بهطور معنی</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">داری بالاتر از گروه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">DC</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> (04/0 ></span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">)</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">و</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">DVD</span></span></span></span> <span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> (005/0 ></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">) بود. همچنین در گروه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">DAT</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> بهطور معنی</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">داری بالاتر از گروه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">DVD</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> بود (018/0 ></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">). شاخص مقاومت به انسولین نیز در گروه</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">های</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">DVD</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">، </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">DAT</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">DATVD</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> بهطور معنی</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="letter-spacing:-.1pt"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">داری کمتر از گروه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">DC</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> بود (001/0 ></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">).</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">نتیجهگیری</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">: بهنظر می</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">رسد که هشت هفته تمرین هوازی همراه با مکمل</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">دهی ویتامین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">D3</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> از طریق افزایش پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">GLUT4</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> و بهبود مقاومت به انسولین منجر به بهبود متابولیسم گلوکز در موش</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">های دیابتی می</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">شود.</span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Background:</span></b><span new="" roman="" style="font-family:" times=""> Glucose transporter4 (GLUT4) is the main glucose transporter in skeletal muscle. Impaired GLUT4 expression plays a role in the disorders of glycemic homeostasis. The aim of the present study was to investigate the combined effects of aerobic training and vitamin D3 supplementation on Glut4 protein levels and insulin resistance in the soleus muscle of diabetic rats with STZ and high-fat diet. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In 40 male Wistar rats type 2 diabetes was induced by 6 weeks high-fat diet followed by streptozotocin injection. Then rats were randomly divided into five groups: Healthy control (HC), Diabetic control (DC). Diabetes+Aerobic training (DAT), Diabetes+Vitamin D3 (DVD) and Diabetes+Aerobic training+ Vitamin D3 (DVDAT). The rats underwent eight weeks of aerobic training and vitamin D3 supplementation. 24h after last session of training and, the rats were anesthetized and soleus muscle was isolated for measurement of Glut4 protein concentrations and serum levels of insulin, glucose, vitamin D3 index were measured.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><span new="" roman="" style="font-family:" times=""> One-way ANOVA showed that GLUT4 protein levels in DC group was significantly lower than HC group (P<0.001), but in DVDAT group was significantly higher than DC group (P<0.04) and DVD group (P<0.005). Also in DAT group was significantly higher than DVD (P<0.018). The HOMA-IR index also in DVDAT, DAT and DVD groups was significantly lower than DC group (P<0.001).</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><span new="" roman="" style="font-family:" times=""> It seems that Eight weeks of aerobic training with vitamin D3 supplementation improves glucose metabolism in diabetic rats via increasing Glut4 protein levels and improving insulin resistance index.</span></span></span></span></span>
تمرین هوازی, ویتامین D3, پروتئین GLUT4, مقاومت به انسولین, موش دیابتی
Aerobic Training, Vitamin D3, Glut4 Protein, Insulin Resistance, Diabetic Rat
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