Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
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fa
jalali
1403
7
1
gregorian
2024
10
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4
online
1
fulltext
fa
تغییرات بیان ژن miR-17 و شاخص های استرس اکسیداتیو در زنان دارای اضافه وزن و چاق به دنبال تمرین های عملکردی با شدت بالا
Changes in Expression of miR-17 Gene and Oxidative Stress Indices in Overweight and Obese Women Following High-Intensity Functional Training
عمومى
General
پژوهشي
Research
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">مقدمه:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> اثرات ورزش منظم بر پروفایل </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miRNA</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و شاخصهای استرس اکسیداتیو کمتر شناخته شده است. هدف از مطالعۀ حاضر بررسی تغییرات بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">mRNA17</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و شاخصهای استرس اکسیداتیو در زنان دارای اضافه وزن و چاق بهدنبال تمرین</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">های</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> عملکردی با شدت بالا بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">روشها:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> در این مطالعۀ نیمهتجربی،22 زن دارای اضافه وزن و چاق بهصورت تصادفی در دو گروه تمرین عملکردی با شدت بالا (10 نفر) و کنترل (12 نفر) تقسیم شدند. آزمودنیهای گروه تمرینی 3 جلسه در هفته بهمدت 8 هفته به اجرای تمرین</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">های</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> عملکردی با شدت بالا (مقیاس درک فشار بالاتر و مساوی 7) پرداختند. قبل و پس از 8 هفته، نمونههای خونی جهت اندازهگیری بیان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miR-17</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">، مالون دی آلدئید (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MDA</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)، سوپراکسیددیسموتاز، گلوتاتیون پراکسیداز (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">GPx</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) و ظرفیت آنتیاکسیدانی تام (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TAC</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) به عمل آمد. از آزمون تحلیل کواریانس جهت تجزیهوتحلیل دادهها استفاده شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">یافتهها:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">بعد از هشت هفته تمرینهای عملکردی با شدت بالا، بیان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">miR-17</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">در گروه تجربی نسبت به گروه کنترل بهطور معنیداری کاهش یافت</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">(001/0 =</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">. بعد از هشت هفته تمرینهای عملکردی با شدت بالا </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">TAC</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">(0001/0 =</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">GPX</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">(007/0 =</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">SOD</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">(<span style="letter-spacing:-.1pt">001/0 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">) </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">در گروه تجربی نسبت به گروه کنترل بهطور معنیداری افزایش یافت. تغییر معناداری در </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">MDA</span></span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> مشاهده نشد </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">(778/0 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">)</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتیجهگیری:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> نتایج مطالعۀ حاضر نشان داد هشت هفته تمرین عملکردی با شدت بالا ممکن است منجر به کاهش بیان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miR-17</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و بهبود شاخصهای استرس اکسیداتیو و آنزیمهای آنتیاکسیدانی گردد که احتمال دارد در بهبود شاخصهای متابولیک و عوارض چاقی مؤثر باشد.</span></span></span></span></span></span></span></span>
<b><span new="" roman="" style="font-family:" times="">Background:</span></b> <span new="" roman="" style="font-family:" times="">The effects of regular exercise on miRNA profile and oxidative stress indices are less known. The aim of this study was to investigate the changes in expression of miR-17 and oxidative stress indices in overweight and obese women following high-intensity functional training.</span><br>
<b><span new="" roman="" style="font-family:" times="">Methods</span></b><span new="" roman="" style="font-family:" times="">: In this semi-experimental study, 22 overweight and obese women were randomly divided into two groups of high-intensity functional training (n= 10) and control (n= 12). The subjects of the training group performed high-intensity functional exercises 3 times a week for 8 weeks and intensity of </span><span style="background:white"><span new="" roman="" style="font-family:" times="">rating of perceived exertion</span></span><span style="background:white"><span new="" roman="" style="font-family:" times="">≥</span></span><span style="background:white"><span new="" roman="" style="font-family:" times="">7</span></span><span new="" roman="" style="font-family:" times="">. Before and after 8 weeks, blood samples were taken to measure expression of miR-17 gene, malondialdehyde (MDA), superoxide dismutase, glutathione peroxidase (GPx) and total antioxidant capacity (TAC). Analysis of covariance test was used to analyze the data.</span><br>
<b><span new="" roman="" style="font-family:" times="">Results:</span></b> <span new="" roman="" style="font-family:" times="">After eight weeks of high-intensity functional training, expression of miR-17 in the experimental group significantly decreased compared to the control group (P= 0.001). After eight weeks of high-intensity functional training, TAC (P= 0.0001), GPX (P= 0.007) and SOD (P= 0.001) increased significantly in the experimental group compared to the control group. No significant change was observed in MDA (P= 0.778).</span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Conclusion: </span></b><span new="" roman="" style="font-family:" times="">The results of the present study showed that eight weeks of high-intensity functional training may lead to the reduction of miR-17 expression and the improvement of oxidative stress indices and antioxidant enzymes, which may be effective in improving metabolic indices and obesity complications.</span></span></span></span>
miR-17, استرس اکسیداتیو, چاقی, تمرینهای عملکردی
miR-17, Oxidative Stress, Obesity, Functional Training
199
209
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-468-3&slc_lang=fa&sid=1
Hamid Reza
Zolfi
حمیدرضا
زلفی