fa تأثیر هم‌زمان تمرینهای تناوبی با شدت بالا (HIIT) و رژیم غذایی پُرچرب بر TGF-β1 و MMP-9 و MMP-2 در بافت چربی زیرپوستی رت‌های نر تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> فیبروز بافت چربی در اختلال متابولیسم گلوکز و مقاومت به انسولین در چاقی نقش دارد؛ اما تأثیر ورزش بر پیشرفت فیبروز بافت چربی هنوز ناشناخته است. این مطالعه با هدف بررسی تأثیر هم‌زمان تمرین</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="color:black">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">های تناوبی با شدت بالا و رژیم غذایی پُرچرب بر </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">TGF-&beta;1</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MMP-9</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MMP-2</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در بافت چربی زیرپوستی رت</span></span></span><span lang="FA" style="font-size:12.0pt"><span cambria="" style="font-family:"><span style="color:black">‌</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">های نر انجام شد.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">روش‌ها:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">سر رت نر نژاد ویستار به‌صورت تصادفی به 4 گروه، رژیم غذایی معمولی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ND</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)، رژیم غذایی پُرچرب (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HFD</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">)، رژیم غذایی معمولی+ تمرین‌های تناوبی با شدت بالا (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ND+HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) و رژیم غذایی پُرچرب‌+تمرین‌های تناوبی با شدت بالا (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HFD+HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) تقسیم شدند. پروتکل </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> شامل هشت مرحله فعالیت با شدت معادل 90 درصد حداکثر ظرفیت دویدن و دورۀ استراحت فعال 5/2 دقیقه‌ای با شدت معادل 50درصد </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MRC</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> به‌مدت 12 هفته (5 جلسه در هفته) بود. 48 ساعت پس از آخرین جلسه تمرین، نمونه</span></span><span lang="FA" style="font-size:12.0pt"><span cambria="" style="font-family:">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">های چربی زیرپوستی و خون جمع‌آوری شدند. برای اندازه‌گیری میزان بیان پروتئین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TGF-&beta;1</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> از روش وسترن بلات و برای اندازه‌گیری </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MMP-9</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MMP-2</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">، انسولین از روش الایزا استفاده شد.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">یافته‌ها:</span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> نتایج نشان داد القای چاقی با افزایش معنی‌دار میزان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">TGF-&beta;1</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">،</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MMP-9 </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">، </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MMP-2</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">، مقاومت به انسولین همراه بود (0001/0</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">< </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">) و در مقابل تمرین تناوبی با شدت بالا به همراه رژیم غذایی پُرچرب نسبت به گروه رژیم غذایی پُرچرب موجب کاهش معنی‌دار میزان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">TGF-&beta;1</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">،</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MMP-9 </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">، </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MMP-2</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">، مقاومت به انسولین می</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="color:black">‌</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">شود (0001/0</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">< </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">).</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">نتیجه‌گیری:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">تمرین تناوبی با شدت بالا، ممکن است پیشرفت فیبروز بافت چربی را تضعیف و اثر پیشگیرانه بر افزایش اختلالات متابولیسم گلوکز ناشی از رژیم غذایی پُرچرب داشته باشد.</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Background:</span></b><span new="" roman="" style="font-family:" times=""> Adipose tissue fibrosis is involved in glucose metabolism disorder and insulin resistance in obesity, but the effect of exercise on the progression of adipose tissue fibrosis is still unknown.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><span new="" roman="" style="font-family:" times="">This study aimed to investigate the effect of high intensity interval training (HIIT) simultaneously with high-fat diet on TGF-&beta;1, MMP-9 and MMP-2 in the subcutaneous adipose tissue of male rats.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">24 male rats were randomly divided into 4 groups: normal diet (ND), high fat diet (HFD), Normal diet + high intensity interval training (ND+HIIT), high fat diet+ high intensity interval training (HFD+HIIT). The HIIT protocol includes 8 bouts of intense activity at 90% of maximum running capacity (MRC) for 2.5 minutes, with active rest periods at 50% of maximum running capacity for 2.5 minutes for 12 weeks (5 sessions per week). 48 hours after the last training session, blood was taken, and subcutaneous fat was removed. Western blot method was used evaluate the TGF-&beta;1 and ELISA method was used to measure levels of MMP-9, MMP-2, insulin.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Results:</span></b> <span new="" roman="" style="font-family:" times="">Induction of obesity was associated with a significant increase in TGF-&beta;1, MMP-9 and MMP-2 and insulin resistance (P˂ 0.0001). In contrast, high-intensity interval with high fat diet compared to the high fat diet group causes a significant decrease in the amount of TGF-&beta;1, MMP-9, MMP-2, and insulin resistance (P˂ 0.0001).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b> <span new="" roman="" style="font-family:" times="">In conclusion, our data indicate that High-intensity interval training may weaken the progression of adipose tissue fibrosis and have a preventive effect on the increase in glucose metabolism disorders caused by a high-fat diet.</span></span></span></span></span> فیبروز بافت چربی, ماتریکس متالوپروتئیناز, تمرینهای تناوبی با شدت بالا, مقاومت به انسولیننسولین Adipose Tissue Fibrosis, Matrix Metalloproteinase, High Intensity Interval Training, Insulin Resistance 245 256 http://ijdld.tums.ac.ir/browse.php?a_code=A-10-2088-1&slc_lang=fa&sid=1 Vida Hakimi ویدا حکیمی 0009-0004-5545-666X No Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran گروه فیزیولوژی ورزشی، دانشکده ی تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران Hamid Mohebbi حمید محبی Mohebbi_h@yahoo.com 0000-0001-9858-5964 Yes Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran گروه فیزیولوژی ورزشی، دانشکده ی تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران Bahman Mirzaei بهمن میرزایی 0000-0003-3723-7434 No Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran گروه فیزیولوژی ورزشی، دانشکده ی تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران