fa اثر مصرف عصارۀ زعفران در خلال تمرین مقاومتی بر بیان پروتئین BDNF هایپوکامپ موش‌های دارای دیابت نوع دو عمومى General كاربردي Applicable <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">مقدمه</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">: </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">اثرات سودمند عصارۀ زعفران و تمرین مقاومتی بر بهبود برخی از شاخص</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span cambria="" style="font-family:"><span style="color:black">‌</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">های دیابت مشخص شده است، اما اثر ترکیبی آنها بر سطوح پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">BDNF</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در موش</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span cambria="" style="font-family:"><span style="color:black">‌</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">های دارای دیابت نوع دو بررسی نشده است. بنابراین، هدف مطالعۀ حاضر بررسی اثر مصرف عصارۀ زعفران در خلال تمرین مقاومتی بر بیان پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">BDNF</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> هایپوکمپ موش</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span cambria="" style="font-family:"><span style="color:black">‌</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">های دارای دیابت نوع دو بود.</span></span></span><b><span dir="LTR" lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span b="" lang="AR-SA" lotus="" style="font-family:">روش‌ها: </span></b><span b="" lang="FA" lotus="" style="font-family:">در این مطالعۀ تجربی 30 سر موش صحرایی نر بالغ به</span><b><span cambria="" dir="LTR" lang="X-NONE" style="font-family:">‌</span></b><span b="" lang="FA" lotus="" style="font-family:">طور تصادفی به پنج گروه کنترل (</span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">C</span><span b="" lang="FA" lotus="" style="font-family:">)، دیابت (</span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">D</span><span b="" lang="FA" lotus="" style="font-family:">)، دیابت-تمرین مقاومتی (</span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">DT</span><span b="" lang="FA" lotus="" style="font-family:">)، دیابت-عصارۀ زعفران (</span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">DS</span><span b="" lang="FA" lotus="" style="font-family:">) و دیابت-عصارۀ زعفران-تمرین مقاومتی (</span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">DTS</span><span b="" lang="FA" lotus="" style="font-family:">) تقسیم شدند. تمرین مقاومتی به</span><b><span cambria="" dir="LTR" lang="X-NONE" style="font-family:">‌</span></b><span b="" lang="FA" lotus="" style="font-family:">مدت شش هفته و پنج جلسه در هفته انجام شد. سطوح گلوکز خون و سطوح پروتئین </span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">BDNF</span><span b="" lang="FA" lotus="" style="font-family:"> هایپوکمپ اندازه</span><b><span cambria="" dir="LTR" lang="X-NONE" style="font-family:">‌</span></b><span b="" lang="FA" lotus="" style="font-family:">گیری شد. برای تحلیل داده</span><b><span cambria="" dir="LTR" lang="X-NONE" style="font-family:">‌</span></b><span b="" lang="FA" lotus="" style="font-family:">ها از آنالیز واریانس یک</span><b><span cambria="" dir="LTR" lang="X-NONE" style="font-family:">‌</span></b><span b="" lang="FA" lotus="" style="font-family:">طرفه در سطح معناداری 05/0 ></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">P</span><span b="" lang="FA" lotus="" style="font-family:"> استفاده شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span b="" lang="FA" lotus="" style="font-family:">یافته‌ها: </span></b><span b="" lang="FA" lotus="" style="font-family:">نتایج نشان داد القای دیابت با </span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">STZ</span> <span b="" lang="FA" lotus="" style="font-family:">همراه با رژیم غذایی پُرچرب باعث تفاوت معنادار در میزان بیان ژن آتروژین-1 بین دو گروه </span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">DM</span> <span b="" lang="FA" lotus="" style="font-family:">با گروه </span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">ARDM</span> <span b="" lang="FA" lotus="" style="font-family:">شد (02/0 =</span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">P</span><span b="" lang="FA" lotus="" style="font-family:">)، درحالی‌که سطح تغییرات بیان ژنی </span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">Foxo1</span> <span b="" lang="FA" lotus="" style="font-family:">بین گروه </span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">ARDM</span> <span b="" lang="FA" lotus="" style="font-family:">با دیگر گروه</span><span cambria="" lang="FA" style="font-family:">‌</span><span b="" lang="FA" lotus="" style="font-family:">ها معنادار بود (001/0=</span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt">P</span><span b="" lang="FA" lotus="" style="font-family:">).</span><span lang="AR-SA" style="font-family:"B Lotus""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:95%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">نتیجه‌گیری: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">انجام تمرین مقاومتی همچنین مصرف عصارۀ زعفران به</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:">‌</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">تنهایی و همراه با تمرین مقاومتی سطوح گلوکز خون را در موش</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:">‌</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">های دیابتی کاهش می</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:">‌</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">دهد. امّا، مصرف عصارۀ زعفران تنها در خلال تمرین مقاومتی توانست سطوح پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span new="" roman="" style="font-family:" times="">BDNF</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:"> را افزایش دهد. بنابراین، برای بهره</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:">‌</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">وری بیشتر پیشنهاد می</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span cambria="" style="font-family:">‌</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span b="" lotus="" style="font-family:">شود عصارۀ زعفران در خلال تمرین مقاومتی مصرف شود.</span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""></span></span></span></b></span></span></span></span></span></span> <span style="font-size:11pt"><span style="tab-stops:467.55pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Background: </span></b><span lang="EN" new="" roman="" style="font-family:" times="">The beneficial effects of saffron extract and resistance training on the improvement of some diabetes indices have been determined, but their combined effect on BDNF protein levels in rats with type 2 diabetes has not been investigated. Therefore, the aim of the present study was to investigate the effect of saffron extract consumption during resistance training on BDNF protein expression in the hippocampus of rats with type 2 diabetes.</span><span style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods: </span></b><span new="" roman="" style="font-family:" times="">In this experimental study, 30 adult male rats were randomly divided into five groups: control (C), diabetes (D), diabetes-resistance training (DT), diabetes-saffron extract (DS) and diabetes-saffron extract- resistance training (DTS). Resistance training performed five sessions/w for six weeks. Blood glucose levels and hippocampus BDNF protein levels were measured. To analyze the data, one-way ANOVA was used at the significance level of P< 0.05.</span><span style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="tab-stops:467.55pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results: </span></b><span new="" roman="" style="font-family:" times="">Blood glucose levels of all experimental groups were significantly higher than C group (P= 0.001). Blood glucose levels in DT, DS and DTS groups were significantly lower than D group (P= 0.001). Also, the blood glucose levels of the DTS group were significantly lower than that of the DT group (P= 0.014). In addition, BDNF protein levels of all experimental groups were significantly lower than group C (P< 0.05). Also, BDNF protein levels of D, DT and DS groups were significantly lower than DTS group (P< 0.05). However, BDNF protein levels of D, DT and DS groups were not significantly different (P< 0.05).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion: </span></b><span new="" roman="" style="font-family:" times="">Performing resistance training also consuming saffron extract alone and during resistance training reduces blood glucose levels in diabetic rats. However, consumption of saffron extract could increase BDNF protein levels only during resistance training. Therefore, for more efficiency, it is suggested to consume saffron extract during resistance training.</span><span style="font-size:12.0pt"><span style="background:yellow"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> &nbsp; دیابت, تمرین مقاومتی, زعفران, BDNF Diabetes, Resistance Training, Saffron, BDNF 302 309 http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1048-7&slc_lang=fa&sid=1 Vahid Valipour Dehnou وحید ولی پور ده نو valipour.v@lu.ac.ir 0000-0001-7049-8164 Yes Sports Sciences Department, Faculty of Literature and Human Sciences, Lorestan University, Khorramabad, Iran گروه علوم ورزشی، دانشکدۀ ادبیات و علوم انسانی، دانشگاه لرستان، خرم‌آباد، ایران