Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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2345-4008
2345-4016
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fa
jalali
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gregorian
2024
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تأثیر یک دوره تمرین استقامتی، بر فیبروز قلبی و آنزیمهای اکسیداتیو موشهای دیابتی نوع دو
The Effect of Endurance Training on Myocardial Fibrosis and Oxidative Enzymes in Type 2 Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">مقدمه:</span></span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"> دیابت نوع دو یک بیماری مزمن متابولیکی </span></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">و</span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> اختلال پیچیده با چندین عارضۀ</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">کوچک و بزرگ</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> عروقی در قسمت</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">های مختلف بدن است که با فیبروز قلبی همراه است<span style="background:white"><span style="color:black">. از طرفی به</span></span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">نظر می</span></span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">رسد تمرین استقامتی با کاهش میزان گلوکز ناشتا و افزایش شاخص</span></span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">های آنتیاکسیدانی موجب پیشگیری از توسعۀ فیبروز قلبی در دیابت میشود.</span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">روشها:</span></span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"> 24 سر موش نر نژاد ویستار پس از آشناسازی با محیط آزمایشگاه بهطور تصادفی به سه گروه سالم کنترل (8 =</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="color:black">NC. n</span></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">) دیابت کنترل </span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">(8 =</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="color:black">DC. n</span></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">) و دیابت ورزش </span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">(8 =</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="color:black">DT. n</span></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">)</span></span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">تقسیم شدند. دیابت از طریق مصرف رژیم غذایی پُرچرب و تزریق </span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">استرپتوزوتوسین<span style="background:white"><span style="color:black"> القا شد. گروههای تمرینی، 8 هفته تمرین تناوبی استقامتی را روی نوارگردان اجرا کردند. </span></span>برای بررسی میزان فیبروز و بههم ریختگی سلولی از رنگ آمیزی هماتوکسیلین ائوزین و ماسون تری کروم استفاده شد. مالون دیآلدهیدسرم (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">MDA</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) توسط اسپکتروفتومتری تیوباربیتوریک اسید اندازهگیری شد. همچنین آنتـیاکـسیدان کل سـرم نیز به روش </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">FRAP</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> سنجیده شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">یافته</span></span></span></b><b> </b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">ها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> موشها در گروه</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تمرین</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">در مقایسه با گروه کنترل دیابتی کاهش در میزان فیبروز، گلوکز ناشتا و همچنین کاهش</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">در تریگلیسرید و کلسترول تام مشاهده شد (05/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">). </span></span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Lotus"">نتیجهگیری:</span></span></span></b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Lotus""> <span style="background:white"><span style="color:black">تمرین استقامتی در بیماران دیابتی با کاهش قند خون ناشتا،</span></span></span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:107%"><span style="font-family:"B Lotus""><span style="color:black">پروفایل لیپیدی و افزایش </span></span></span></span></span><span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:9pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""></span></span></b></span></span></span></span>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Background:</span></b><span style="font-family:"Times New Roman",serif"> Type 2 diabetes is a chronic metabolic disease and a complex disorder with several micro and macro vascular complications in different parts of the body, which is associated with cardiac fibrosis. On the other hand, endurance training seems to prevent the development of cardiac fibrosis in diabetes by reducing fasting glucose levels and increasing antioxidant indices.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Methods:</span></b><span style="font-family:"Times New Roman",serif"> 24 male Wistar rats were randomly divided into three groups: healthy control (NC, n=8), diabetes control (DC, n=8) and exercise diabetes (DT, n=8) after familiarization with the laboratory environment. Diabetes was induced to diabetic animals through streptozotocin injection. Training groups, performed 8 weeks of intermittent endurance training on a treadmill. Hematoxylin-eosin and Masson trichrome staining were used to check the level of fibrosis and cell disorder. Serum malondialdehyde (MDA) was measured by thiobarbituric acid spectrophotometry. Also, total serum antioxidants were measured by FRAP method.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Results:</span></b><span style="font-family:"Times New Roman",serif"> Compared to the diabetic control group, rats in the training group showed a decrease in fibrosis, fasting glucose, and also a decrease in triglyceride and total cholesterol (P<0.05).</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Conclusion:</span></b><span style="font-family:"Times New Roman",serif"> Based on the results, it seems that Endurance training in diabetic Rats prevents the development of cardiac fibrosis caused by diabetes by reducing fasting blood sugar, lipid profile and increasing total antioxidants. However, more studies are needed.</span></span></span></span></span><br>
تمرین استقامتی, فیبروز قلبی, آنزیمهای اکسیداتیو, دیابت نوع دو
Endurance training, Cardiac fibrosis, Oxidative enzymes, Type II diabetes, Rat
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301
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-395-3&slc_lang=fa&sid=1
Mahdi
Faramoushi
مهدی
فراموشی
m.faramoushi@tabriziau.ac.ir