Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
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Medical Sciences
http://ijdld.tums.ac.ir
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2024
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تأثیر تمرین تناوبی شدید بر بیان TCF7L2 کبدی و کنترل گلایسمیک در رتهای دیابتی نوع دو
The Effect of High Intensity Interval Training on Hepatic TCF7L2 Expression and Glycemic Control in Type 2 Diabetes Rats
عمومى
General
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">مقدمه:</span></span></span></span></b> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">TCF7L2</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">یکی از قویترین عوامل خطر ژنتیکی مرتبط با دیابت نوع دو است. هدف این مطالعه بررسی تأثیر 10 هفته تمرین تناوبی شدید بر بیان ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">TCF7L2</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> در سلولهای کبدی موشهای صحرایی دیابتی نوع دو بود.</span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">روشها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> در این مطالعۀ تجربی 14 سر موش صحرایی نر نژاد ویستار </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">با تزریق </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نیکوتین آمید-استرپتوزوتوسین به دیابت نوع دو مبتلا شدند و تصا</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">د</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">فی در دو گروه تمرین (7 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">n=</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> و کنترل (7</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">n= </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">قرار گرفتند. گروه تمرین، برنامۀ تمرینهای تناوبی شدید را </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">بهمدت10 هفته و 5 روز در هفته با دویدن روی تردمیل اجرا کردند.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> 48 ساعت پس از آخرین جلسۀ تمرینی، بیان ژن</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">TCF7L2</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">در کبد،</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">سطح </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">گلوکز</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> خون، </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">انسولین، هموگلوبین گلیکوزیله و مقاومت انسولین در هر دو گروه اندازه گیری شدند. تجزیهوتحلیل دادهها با استفاده از آزمون تی مستقل در سطح 05/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P<</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> انجام شد.</span></span></span><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">یافتهها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> پس از مداخلۀ ورزشی، بیان ژن</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">TCF7L2</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">و سطح سرمی انسولین در گروه تمرین نسبت به گروه کنترل به میزان معنیداری بالاتر بودند (05/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P<</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">).<b> </b>سطح گلوکز خون، هموگلوبین گلیکوزیله و مقاومت انسولین در گروه تمرین به میزان معنیداری پایینتر از گروه کنترل بودند (05/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P<</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">).</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتیجهگیری:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> بر اساس این یافتهها، بهنظر میرسد بهبود کنترل گلایسمیک در پاسخ به تمرینهای تناوبی شدید تا اندازهای به کاهش گلوکونئوژنز ناشی از افزایش بیان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">TCF7L2</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> کبدی وابسته است.</span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Background:</span></b><span style="font-family:"Times New Roman","serif""> Transcription factor 7-like 2 (TCF7L2) is one of the strongest genetic risk factors associated with type 2 diabetes mellitus (T2DM). The purpose of this study was to investigate the effect of 10 weeks of high intensity interval training (HIIT) on hepatic TCF7L2 expression in T2DM rats. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Methods:</span></b><span style="font-family:"Times New Roman","serif""> In this experimental study, fourteen males Wistar rats with T2DM induced by streptozotocin-nicotinamide injection were randomly divided into exercise (n=7) and control (n=7) groups. The exercise group performed the HIIT protocol on a treadmill for 10 weeks and 5 days per week. Hepatic TCF7L2 expression, blood glucose level, hemoglobin A1c (HbA1c), insulin and insulin resistance were measured 48 hours after the last training session. Data were analyzed by independent T test at P< 0.05.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Results:</span></b><span style="font-family:"Times New Roman","serif""> Hepatic TCF7L2 expression and serum insulin were significantly higher in the exercise group compared to the control group (P˂0.05). Blood glucose level, HbA1c and insulin resistance were significantly lower in the exercise group than in the control group (P˂0.05). </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Conclusion:</span></b><span style="font-family:"Times New Roman","serif""> Based on these findings, it seems that the improved glycemic control in response to HIIT is partially dependent on the inhibition of gluconeogenesis caused by increased hepatic TCF7L2 expression. </span></span></span></span></span>
تمرین تناوبی, دیابت نوع دو, گلوکونئوژنز, TCF7L2
Interval Training, Type 2 Diabetes, Gluconeogenesis, TCF7L2
170
178
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1681-2&slc_lang=fa&sid=1
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Parsayyrad
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