Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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2345-4008
2345-4016
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fa
jalali
1403
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2024
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تأثیر تمرین تناوبی با شدت متوسط (MIIT) بر محتوای تام و فسفریله پروتئین AMPKα1/2 در عضلۀ اسکلتی نعلی موشهای صحرایی دیابتی
The Effect of Moderate Intensity Interval Training (MIIT) on the Total and Phosphorylated Content of Ampkα1/2 Protein in the Soleus Skeletal Muscle of Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:300.45pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">مقدمه:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> پروتئین کیناز فعال شده با آدنوزین مونوفسفات (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">AMPK</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) یک تنظیمکنندۀ کلیدی متابولیسم سلولی است و اختلال در تنظیم آن با بیماریهای متابولیک مانند چاقی، التهاب، دیابت و سرطان مرتبط است. هدف از انجام تحقیق حاضر، تأثیر تمرین تناوبی با شدت متوسط (</span></span></span><span dir="LTR" lang="EN-GB" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">MIIT</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) بر محتوای تام و فسفریله پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">AMPKα1/2</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">در عضلۀ اسکلتی </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">موشهای صحرایی دیابتی است.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">روشها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> <span style="letter-spacing:-.2pt">در این مطالعۀ تجربی، 12 سر رت نر دو ماهه از نژاد اسپراگداولی با میانگین وزن 30</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria",serif"><span style="letter-spacing:-.2pt">±</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">280 گرم انتخاب شدند. دیابت از طریق تزریق درونصفاقی </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">با </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">محلول </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">استرپتوزوتوسین (با دوز 65 میلی</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">گرم به ازای هر کیلوگرم از وزن بدن) به رتها القاء <span style="letter-spacing:-.2pt">شد. این رتها به روش تصادفی به گروه تمرین دیابتی و کنترل دیابتی</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">تقسیم شدند؛ گروه تمرین،</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt">MIIT</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> را به مدت 6 هفته با شدتی معادل 55 تا 75 درصد </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">حداکثر سرعت انجام دادند. تجزیه</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">و</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">تحلیل دادهها از طریق آزمون </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt">t</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">-مستقل </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">در نرمافزار گرافپد پریسم نسخۀ 10 </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">انجام </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">شد.</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">سطح معناداری پژوهش 05/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">≤ </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> در نظر گرفته شد.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:dotted 0cm"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">یافتهها: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">محتوای درون سلولی تام پروتئین</span></span></span><span dir="LTR" lang="EN-GB" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">AMPKα1/2</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تغییر معنیداری را در گروه تمرین نسبت به گروه کنترل در عضلۀ اسکلتی نعلی نشان نداد (96/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">= </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">). در مقابل محتوای درون سلولی فسفریله (0001/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">= </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) و نسبت فسفریله به تام (002/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">= </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) پروتئین</span></span></span><span dir="LTR" lang="EN-GB" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">AMPKα1/2 </span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">افزایش معنیداری را نشان داد.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتیجهگیری:</span></span></span></b> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">MIIT</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> محتوای پروتئین </span></span></span><span dir="LTR" lang="EN-GB" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">AMPKα1/2</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">را در بافت عضلانی نعلی موشهای دیابتی افزایش داد و این میتواند منجر به افزایش تولید و مصرف انرژی و بهبود سطوح گلوکز در آزمودنیهای دیابتی شود.</span></span></span></span></span></span></span></span>
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<div id="ftn1"><span style="font-size:10pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span lang="FA" dir="RTL" style="font-family:"Arial",sans-serif"></span></span></span></span></div>
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<span style="font-size:11pt"><span style="line-height:92%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Background:</span></b><span style="font-family:"Times New Roman",serif"> Adenosine monophosphate-activated kinase (AMPK) is a key regulator of cellular metabolism, and its dysregulation is associated with metabolic diseases such as obesity, inflammation, diabetes, and cancer. Therefore, the purpose of this research is the effect of moderate intensity interval training (MIIT) on the total and phosphorylated content of AMPKα1/2 protein in the skeletal muscle of diabetic rats.</span><span dir="RTL" lang="AR-SA" style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:dotted 0cm"><span style="text-autospace:none"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Methods</span></b><span style="font-family:"Times New Roman",serif">: In this experimental study, 12 two-month-old male Sprague Dawley rats with an average weight of 280±30 grams were selected. Diabetes was induced to rats through intraperitoneal injection of streptozotocin solution (with a dose of 65 mg per kg of body weight). These rats were randomly divided into two groups, diabetic training and diabetic control; The training group performed MIIT for 6 weeks at an intensity equal to 55-75% of maximum speed. Data analysis was done through independent t-test in GraphPad Prism version 10 software. The significance level of the current research is P≤ 0.05.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:dotted 0cm"><span style="text-autospace:none"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Results</span></b><span style="font-family:"Times New Roman",serif">: Total intracellular content of AMPKα1/2 protein did not show significant changes in the training group compared to the control group in the soleus skeletal muscle (P= 0.96). In contrast, the phosphorylated intracellular content (P= 0.0001) and the ratio of phosphorylated to total form (P= 0.002) of AMPKα1/2 protein showed a significant increase.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Conclusion</span></b><span style="font-family:"Times New Roman",serif">: MIIT increased the protein content of AMPKα1/2 in soleus muscle tissue of diabetic rats, and this could lead to increased energy production and consumption and improved glucose levels in diabetic subjects.</span></span></span></span><br>
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<span style="font-size:11pt"><span style="line-height:92%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Keywords:</span></b><span style="font-family:"Times New Roman",serif"> Adenosine Monophosphate-activated Kinase (AMPKα1/2), Moderate Intensity Interval Training, Soleus Muscle, Diabetes</span><span lang="FA" dir="RTL" style="font-family:"Times New Roman",serif"></span></span></span></span><br>
پروتئین کیناز فعال شده با آدنوزین مونوفسفات (AMPKα1/2), تمرین تناوبی با شدت متوسط, عضله نعلی, دیابت
Adenosine Monophosphate-activated Kinase (AMPKα1/2), Moderate Intensity Interval Training, Soleus Muscle, Diabetes
310
318
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1699-2&slc_lang=fa&sid=1
Sajad
Mirzaei
سجاد
میرزایی