Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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2345-4008
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jalali
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2025
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تأثیر تمرین تناوبی با شدت متوسط (MIIT) بر محتوای تام و فسفوریله پروتئین ریبوزومی S6K1 در بافت قلب رتهای دیابتی نوع دو
Effect of Moderate-Intensity Interval Training (MIIT) on Total and Phosphorylated Content of Ribosomal Protein S6K1 in Heart Tissue of Type 2 Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">مقدمه:</span></span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"> دیابت نوع 2 با مقاومت به انسولین و قند خون مشخص میشود و میتواند منجر به بیماری قلبی شود. بنابراین، هدف از مطالعه حاضر بررسی تأثیر </span></span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="color:black">MIIT</span></span></span></span></span><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"> بر مسیر </span></span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="color:black">S6K1</span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"> در میوکارد است که مربوط به کنترل رشد و تکثیر سلول است.</span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:dotted 0cm"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">روشها: </span></span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">در این مطالعه، 12 موش صحرایی نر دو ماهه با نژاد اسپراگوداولی و وزن متوسط </span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"Arial",sans-serif"><span style="color:black"></span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">30 </span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="color:black">±</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"> 280 گرم شرکت کردند. برای القاء دیابت، محلولهای نیکوتین آمید و استرپتوزوتوسین به ترتیب با دوز 110 میلی گرم بر کیلوگرم و 60 میلی گرم بر کیلوگرم تزریق شدند. قند خون موش بین 126-260 میلی گرم در دسی لیتر به عنوان شاخص دیابت نوع 2 تعیین شد. پس از القای دیابت، موشها به طور تصادفی به گروه تمرین دیابتی (6 سر) و گروه کنترل دیابتی (6 سر) تقسیم شدند. گروه تمرین دیابتی هر هفته به مدت 4 هفته و 4 جلسه تمرین کردند. 24 ساعت پس از آخرین جلسه تمرین، بطن چپ قلب جدا شد و میزان پروتئین با روش وسترن بلات اندازهگیری شد. متغیرها از طریق آزمونهای </span></span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"><span style="color:black">T</span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black"> مستقل تحلیل شدند. سطح معنیداری مطالعه 05/0 در نظر گرفته شد.<b> </b></span></span></span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:dotted 0cm"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">یافته</span></span></span></span></span></b><b> </b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="color:black">ها:</span></span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> بررسی دادهها نشان داد محتوای درون سلولی تام (62/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">=</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)، فسفوریله (85/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">=</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) و نسبت تام به فسفوریله (77/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">=</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">S6K1</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">پس از 4 هفته </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">MIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">، تغییر معنیداری نشان نمیدهد.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:dotted 0cm"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتیجهگیری:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> بهنظر میرسد پس از 4 هفته </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">MIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">، پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">S6K1</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">تغییر معنیداری نمیکند. از اینرو باید مدت و شدت تمرین و شرایط تغذیه برای افزایش فسفوریلاسیون </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif">S6K1</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> در تحقیقات آینده مدنظر قرار گیرد. </span></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Background:</span></b><span style="font-family:"Times New Roman",serif"> Type 2 diabetes is characterized by insulin resistance and hyperglycemia and can lead to heart disease. Therefore, the aim of the present study is to investigate the effect of MIIT on the S6K1 pathway in the myocardium, which is related to the control of cell growth and proliferation.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Methods:</span></b><span style="font-family:"Times New Roman",serif"> In this study, 12 two-month-old male Sprague Dawley rats with an average weight of 280±30 grams participated. To induce diabetes, nicotinamide and streptozotocin solutions were injected with a dose of 110 mg/kg and 60 mg/kg, respectively. The blood sugar of rats was determined between 126-260 mg/dL as an indicator of type 2 diabetes. After the induction of diabetes, the rats were randomly divided into diabetic training group (6 heads) and diabetic control group (6 heads). The diabetic training group trained for 4 weeks and 4 sessions every week. 24 hours after the last training session, the left ventricle of heart was isolated and the amount of protein was measured by western blotting method. Variables were analyzed through independent t-tests. The significance level of study was considered P≤0.05.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Results: </span></b><span style="font-family:"Times New Roman",serif">Data analysis showed that the intracellular content of total (P=0.62), phosphorylated (P=0.85), and total to phosphorylated (P=0.77) S6K1 protein did not show significant changes after 4 weeks of MIIT. </span></span></span></span></span><br>
<b><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif">Conclusion:</span></span></span></b><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif"> It seems that after 4 weeks of MIIT, S6K1 protein does not change significantly, so it seems that the duration and intensity of training and nutritional conditions to increase S6K1 phosphorylation should be considered in future research</span></span></span>
تمرین تناوبی با شدت متوسط (MIIT), پروتئین ریبوزومی S6K1, قلب, دیابت نوع دو
Moderate-intensity interval training (MIIT), Ribosomal protein S6K1, Heart, Type 2 diabetes
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1699-3&slc_lang=fa&sid=1
Hamed
Alizadeh pahlavani
حامد
علیزاده پهلوانی
ha.alizadeh@cfu.ac.ir