Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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2345-4008
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jalali
1403
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2024
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تأثیر هشت هفته تمرینهای تناوبی با شدت بالا بر بیان ژن Nrf2، پراکسیداسیون لیپیدی و مقاومت به انسولین در پانکراس موشهای صحرایی مسن تغذیهشده با غذای پُرچرب
The Effects of Eight Weeks of High-Intensity Interval Training (HIIT) on the Nrf2 Gene Expression, Lipid Peroxidation and Insulin Resistance in Pancreas Tissue of Aged Rats Fed a High-Fat Diet
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">مقدمه</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">: افزایش سن و مصرف رژیم غذایی پُرچرب منجر به افزایش آسیب اکسیداتیو بافتهای مختلف میشود، استرس اکسیداتیو یک عامل بحرانی در روند پیری است که میتواند باعث آسیب مستقیم به ساختار سلولی شود هدف این پژوهش بررسی تأثیر هشت هفته تمرین</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">های</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> تناوبی با شدت بالا بر بیان ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Nrf2</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">، پراکسیداسیون لیپیدی و مقاومت به انسولین در بافت پانکراس موشهای صحرایی مسن تغذیهشده با غذای پُرچرب است.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">روشها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">در این تحقیق تجربی، </span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">20 موش صحرایی نر ویستار مسن (سن: 18ماه و میانگین وزن70</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:">±</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">450 گرم) بهطور تصادفی در چهار گروه شامل کنترل غذای نرمال </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">G1</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> (5</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">n</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">=</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، غذای نرمال+تمرین</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">G2</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> (5</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">n=</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، غذای پُرچرب </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">G3</span></span></span> <span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">(5</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">n</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">=</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">و</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> غذای پُرچرب+تمرین</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">G4</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> (5</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">n</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">=</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، قرار گرفتند. برنامۀ تمرین </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">تناوبی با شدت بالا </span></span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">روی تردمیل سه روز در هفته و بهمدت هشت هفته انجام شد. بیان ژن </span></span></span><span class="fontstyle01" serif="" style="font-family:BLotusBold,"><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="font-weight:normal">فاکتور رونویسی</span></span></span></span></span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Nrf2</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> با استفاده از </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Real-time PCR</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> انجام شد و </span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">مالون دیآلدهید، گلوکز و انسولین با استفاده از کیت و به روش الایزا اندازهگیری شد. دادهها با آزمون آماری </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">MANOVA</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> در سطح معنیداری </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P< 0.05</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> تجزیهوتحلیل شد.</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">یافتهها</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">: <span style="letter-spacing:-.1pt">نتایج آزمون آماری </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">MANOVA</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> در خصوص اثر تعاملی تمرین و رژیم غذایی نشان داد که در شاخص مقاومت به انسولین (017/0 =</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> و 17/7 =</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">F</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">) تفاوت معنیدار وجود دارد. اما برای فاکتور انسولین (30/0 =</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> و 13/1 =</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">F</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">)، گلوکز (116/0 =</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> و 75/2 =</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">F</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">)، </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">MDA</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> (87/0 =</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> و 028/0 =</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">F</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">) و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Nrf2</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> (816/0 =</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">P</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> و 056/0 =</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">F</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">) تأثیر معنیداری مشاهده نگردید.</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">نتیجهگیری</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">: بهطورکلی میتوان بیان نمود که تمرین تناوبی با شدت بالا در این تحقیق با تأثیر بر بیان ژن فاکتور رونویسی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Nrf2</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:"> میتواند باعث بهبود در مقاومت انسولینی از طریق کاهش فعالیت اکسیدانی در موشهای صحرایی سالمند باشد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span style="color:black"><span dir="LTR" style="font-size:12.0pt"><span style="background:yellow"><span style="line-height:107%"><span arial="" style="font-family:"><span style="color:#010205"></span></span></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Background:</span></b><span new="" roman="" style="font-family:" times=""> Aging and consuming a high-fat diet lead to increased oxidative damage to various tissues, oxidative stress is a critical factor in the aging process that can cause direct damage to cellular structure. This study aimed to investigate the Effects of 8 Weeks of HIIT Training on the Nrf2 Gene Expression, lipid peroxidation and Insulin resistance in the pancreas tissue of Aged rats fed a high-fat diet.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Methods: </span></b><span new="" roman="" style="font-family:" times="">In this experimental study, 20 aged male Wistar rats (age: 18 months and mean weight: 500±100 gr) were randomly divided into four groups including normal food control G1 (n=5), normal food + training G2 (n=5), high-fat food G3 (n=5) and high-fat food +training G4 (n=5). The high intensity interval training program was performed on a treadmill, three days a week for eight weeks. Nrf2 gene expression was performed using real-time PCR and malondialdehyde levels, glucose and insulin were measured using a kit and ELISA method. Data were analyzed by MANOVA test at the P<0.05.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><span new="" roman="" style="font-family:" times=""> The results of the MANOVA statistical test on the interactive effect of training and diet indicated a significant difference in the insulin resistance index (P = 0.017 and F = 7.17). However, no significant effect was observed for the insulin factor (P = 0.30 and F = 1.13), glucose (P = 0.116 and F = 2.75), MDA (P = 0.87 and F = 0.028), and Nrf2 (P = 0.816 and F = 0.056).</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><span new="" roman="" style="font-family:" times=""> In general, it can be stated that HIIT training in this research can improve insulin resistance by affecting the expression of the Nrf2 transcription factor gene by reducing the oxidant activity in aged rats.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:150%"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span style="color:black"><span dir="RTL" lang="AR-SA" style="font-size:10.5pt"><span style="line-height:150%"><span style="font-family:"Segoe UI","sans-serif""></span></span></span></span></span></span></span></span>
فاکتور رونویسی Nrf2, سیستم آنتیاکسیدان, غذای پُرچرب, سالمندیندی
Nrf2, Antioxidant System, High-Fat Food, Aging
222
231
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-726-2&slc_lang=fa&sid=1
Yeganeh
Golmohammadi samani
یگانه
گل محمدی سامانی