Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
000
000
000
000
000
fa
jalali
1403
11
1
gregorian
2025
2
1
24
6
online
1
fulltext
fa
تأثیر تمرین تداومی با شدت متوسط (MICT) بر محتوای تام و فسفوریله پروتئین-1- اتصالدهندۀ-4E- فاکتور شروع ترجمۀ یوکاریوتی (4EBP1) در بافت عضلۀ دوقلو رتهای دیابتی نوع یک
The Effect of Moderate-Intensity Continuous Training (MICT) on Total and Phosphorylated Content of Eukaryotic Translation Initiation Factor-4E-Binding Protein-1 (4EBP1) in the Muscle Tissue of Type 1 Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">مقدمه:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> <span style="color:black">دیابت نوع یک با هیپرگلیسمی پایدار مشخص میشود و منجر به اختلال سنتز پروتئین عضلانی و در نهایت تحلیل و کاهش عملکرد عضلانی میشود. </span>بنابراین، این تحقیق با هدف بررسی تأثیر تمرین تداومی با شدت متوسط (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">MICT</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) بر میزان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">4EBP1</span></span></span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">در عضلۀ دوقلو رتهای دیابتی نوع یک انجام شد.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">روشها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> <span style="letter-spacing:-.2pt">پژوهش حاضر از نوع تجربی- بنیادی است که در آن 12 رت نر 2 ماهه از نژاد اسپراگداولی با میانگین وزن 30</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="letter-spacing:-.2pt">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">280 گرم شرکت ‏داشتند. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">برای ایجاد دیابت نوع یک، محلول استرپتوزوتوسین (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">STZ</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">) </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">بهصورت داخل صفاقی </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">با دوز </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">mg/kg</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> 65 </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">تزریق شد. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">سه<span style="color:black"> روز پس از تزریق،</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> قند خون بالای </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">mg/dl</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> 300 بهعنوان شاخص دیابتی شدن نوع یک در نظر گرفته شد. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.4pt">پس از القای دیابت، رتها به روش تصادفی به دو گروه تمرین دیابتی (6 سر) و کنترل دیابتی (6 سر) </span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt">تقسیم شدند.</span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> برنامۀ تمرین تداومی (32 دقیقه با شدت 50-70 درصد حداکثر سرعت) 8 هفته و هر هفته 3 جلسه بود. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">تحلیل دادهها از طریق آزمونهای آماری </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">t</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">-مستقل انجام شد. بررسی دادهها، با استفاده از نرمافزار گرافپد پریسم نسخۀ 2/2/10 انجام گرفت. سطح معناداری پژوهش حاضر، 05/0 </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">≤</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> در نظر گرفته شد.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">یافتهها: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">در گروه تمرین<b> </b></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">پس از 8 هفته </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">MICT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">، محتوای درون سلولی فسفوریله <span style="color:black">(0001/0 </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">=</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">)</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">، تام <span style="color:black">(0001/0 </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">=</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">) </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">و نسبت فسفوریله به تام <span style="color:black">(002/0 </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">=</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">) </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">4EBP1</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">، تغییر معنیداری را نسبت به گروه کنترل در بافت عضلۀ دوقلو نشان داد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">نتیجهگیری: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">بهنظر میرسد</span></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">4EBP1</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">از طریق 8 هفته </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">MICT</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> افزایش مییابند و این سازِکار میتواند سنتز عضله را در بافت دوقلو افزایش دهد.</span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:467.55pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Background: </span></b><span lang="EN" new="" roman="" style="font-family:" times="">Type 1 diabetes is characterized by persistent hyperglycemia and leads to impaired protein synthesis and ultimately muscle breakdown and reduction in muscle function. Therefore, this research was conducted with the aim of investigating the effect of moderate intensity continuous training (MICT) on the amount of 4EBP1 in the biceps muscle of type 1 diabetic rats.</span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods: </span></b><span new="" roman="" style="font-family:" times="">The present study is of experimental-fundamental type in which 12 2-month-old male Sprague Dawley rats with an average weight of 280±30 grams participated. To induce type 1 diabetes, streptozotocin (STZ) solution was injected intraperitoneally at a dose of 65 mg/kg. 3 days after the injection, blood sugar above 300 mg/dl was considered as an indicator of type 1 diabetes. After the induction of diabetes, the rats were randomly divided into 2 diabetic training groups (6 heads) and diabetic control groups (6 heads). The continuous training program (32 minutes with an intensity of 50-70% of maximum speed) was 8 weeks and 3 sessions every week. Data analysis was done through independent t-tests. Data analysis was done using GraphPad Prism software version 10.2.2. The significance level of the present study was considered P≤0.05.</span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:467.55pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results: </span></b><span new="" roman="" style="font-family:" times="">In the training group after 8 weeks of MICT, the intracellular content of phosphorylated (P=0.0001), total (P=0.0001) and the ratio of phosphorylated to total (P=0.002) protein 4EBP1 showed a significant change compared to the control group in the twin muscle tissue.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion: </span></b><span new="" roman="" style="font-family:" times="">4EBP1 protein seems to increase through 8 weeks of MICT and this mechanism can increase muscle synthesis in muscle tissue.</span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span>
تمرین تداومی با شدت متوسط, پروتئین 4EBP1, عضله دوقلو, دیابت نوع یک
Moderate-intensity Continuous Training, 4EBP1 Protein, Gastrocnemius Muscle, Type 1 diabetes
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1699-5&slc_lang=fa&sid=1
Maryamosadat
Miri
مریم السادات
میری