Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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admin
2345-4008
2345-4016
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fa
jalali
1404
1
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gregorian
2025
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online
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fulltext
fa
تأثیر تمرینات مقاومتی بر محور سیگنالینک PI3K/AKT1/mTORc1 و هایپرتروفی قلبی در رتهای چاق دیابتی
The Effect of Resistance Training on PI3K/AKT1/M Torc 1 Signaling Axis and Cardiac Hypertrophy in Diabetic Obese Rats
تخصصي
Special
كاربردي
Applicable
<span style="font-size:11pt"><span style="line-height:90%"><span style="tab-stops:300.45pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span microsoft="" sans="" serif="" style="font-family:"><b><span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">مقدمه:</span></span></b><span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> با توجه به اهمیت ورزش در پیشگیری و درمان بیماریهای مرتبط با چاقی هدف از اجرای مطالعه حاضر تعیین اثر تمرینات مقاومتی بر بیان </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">PI3K,</span></span></span></span> <span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">AKT1</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> و </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">mTORc1</span></span></span></span> <span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">در بافت قلب و هایپرتروفی فیزیولوژیک قلبی رت های دیابتی نوع دو بود.</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span microsoft="" sans="" serif="" style="font-family:"><b><span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">روشها:</span></span></b> <span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">21 رت نر ویستار (10 </span></span><span cambria="" lang="FA" style="font-family:"><span style="color:black">±</span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black"> 220 گرم) توسط 6 هفته رژیم غذایی پُرچرب چاق شدند. بعد از القای چاقی، 14 رت بهواسطۀ تزریق درون صفاقی استرپتوزوتوسین با دوز25میلیگرم بر کیلوگرم (</span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">STZ</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">) دیابتی نوع 2 شدند. نهایتاً رت ها به سه گروه: 1) غیر دیابتی 2) دیابتی کنترل و 3) دیابتی مقاومتی تقسیم شدند. رتهای گروه دیابتی مقاومتی یک دوره تمرینات مقاومتی 6 هفتهای را به تعداد 5 جلسه در هفته کامل کردند. بیان </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">PI3K,</span></span></span></span> <span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">AKT1</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black"> و </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">mTORc1</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black"> در بافت قلب و نسبت وزن بطن چپ به قلب، بطن چپ به بدن و قلب به بدن در همۀ گروهها اندازهگیری و توسط آزمون آنوای یکسویه مقایسه شدند.</span></span><span b="" lang="AR-SA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span microsoft="" sans="" serif="" style="font-family:"><b><span b="" lang="AR-SA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">یافتهها:</span></span></b> <span b="" lang="AR-SA" lotus="" style="font-family:">در پاسخ به القای دیابت، بیان </span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">PI3K, AKT1</span></span></span><span b="" lang="AR-SA" lotus="" style="font-family:"> و </span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">mTORc1</span></span></span><span b="" lang="AR-SA" lotus="" style="font-family:"> نسبت به گروه غیر دیابتی به میزان معنی داری کاهش یافت </span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">(</span></span></span><span dir="LTR" lang="EN" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">P < 0.05</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">)</span></span></span><span b="" lang="AR-SA" lotus="" style="font-family:">. تمرینات مقاومتی به افزایش بیان </span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">PI3K, AKT1</span></span></span><span b="" lang="AR-SA" lotus="" style="font-family:"> و </span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">mTORc1</span></span></span> <span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">(</span></span></span><span dir="LTR" lang="EN" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">P < 0.05</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">)</span></span></span> <span b="" lang="AR-SA" lotus="" style="font-family:">و افزایش نسبت وزن بطن چپ به قلب، بطن چپ به بدن و قلب به بدن در گروه دیابتی مقاومتی نسبت به گروه دیابت کنترل منجر شد</span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">(</span></span></span><span dir="LTR" lang="EN" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">P < 0.05</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times="">) </span></span></span><span b="" lang="AR-SA" lotus="" style="font-family:">.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span microsoft="" sans="" serif="" style="font-family:"><b><span b="" lang="AR-SA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">نتیجهگیری:</span></span></b> <span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">تمرینات مقاومتی با هایپرتروفی فیزیولوژیکی قلب در رت</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">های دیابتی همراه است و این بهبود را می</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">توان به تغییرات در مسیر سیگنالینگ </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">PI3K/AKT1/mTORc1</span></span></span></span> <span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">نسبت داد.</span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:110%"><span microsoft="" sans="" serif="" style="font-family:"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Background:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">The aim of this study was to determine effect of resistance training on the</span></span></span> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">expression of PI3K, AKT1 and mTORc1 in the heart tissue of type 2 diabetes rats and their physiological cardiac hypertrophy.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span microsoft="" sans="" serif="" style="font-family:"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">21 male wistar rats (220</span></span></span><span style="font-size:10.0pt"><span style="line-height:110%">±</span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">20 g) were obese by 6 weeks high fat diet (HFD). After inducing obesity, T2D induced by intraperitoneal injection of streptozotocin (30 mg/kg) in 14 rats. Finally, the rat were randomly assigned to 1) non-diabetes, 2) control T2D, 3) resistance diabetes groups. Rats in the resistance group were completed a resistance</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span microsoft="" sans="" serif="" style="font-family:"><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">training program, 5 sessions weekly for 6 weeks. PI3K, AKT1 and mTORc1 expression and the weight ratio of left ventricular to heart, left ventricular to body and heart to body were compared by ANOVA between groups.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span microsoft="" sans="" serif="" style="font-family:"> <b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">In response to induction of diabetes, the expression of PI3K, AKT1 and mTORc1 in heart tissue decreased significantly compared to the non-diabetic group (p < 0.05). Resistance training resulted in significant increase in the expression of PI3K, AKT1 and mTORc1 and the weight ratio of left ventricular to heart, heart to body and left ventricular to body compared to control diabetes rats (p < 0.05).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span microsoft="" sans="" serif="" style="font-family:"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Resistance training is associated with physiological cardiac hypertrophy in diabetic rats, and this improvement can be attributed to the PI3K/AKT1/mTORc1 signaling pathway.<span style="letter-spacing:-.1pt"></span></span></span></span></span></span></span>
هایپرتروفی فیزیولوژیکی قلب, بیان ژن, تمرین مقاومتی, دیابت
physiological cardiac hypertrophy, Gene expression, Resistance training, Diabetes.
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1905-2&slc_lang=fa&sid=1
Mohammad
Dastyar
محمد
دستیار