Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
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fa
jalali
1404
1
1
gregorian
2025
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fulltext
fa
تأثیر مصرف مکمل منیزیم و تمرین ورزشی بر محتوای پروتئینهای CREB2 و پروتئین همولوگ C/EBP در بطن چپ قلب موشهای صحرایی دیابتی نوع دو
The Effect of Magnesium Supplementation and Training on The Content of CREB2 and C/EBP Homologous Proteins in the Left Ventricle of the Heart of Type 2 Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:90%"><span style="tab-stops:300.45pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span microsoft="" sans="" serif="" style="font-family:"><b><span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">مقدمه:</span></span></b><span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> دیابت میتواند در تعادل مرگ سلولی از طریق مسیرهای سلولی متفاوت اختلال ایجاد کند و تمرینهای ورزشی یا مصرف مکملها میتواند در حفظ تعادل انواع مرگ سلولی مؤثر باشد؛ بنابراین هدف از انجام تحقیق حاضر، تأثیر مصرف مکمل منیزیم و تمرین ورزشی بر محتوای پروتئینهای </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">CREB2</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"> و پروتئین همولوگ </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">C/EBP (CHOP)</span></span></span></span> <span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">در بطن چپ قلب موشهای صحرایی دیابتی نوع دو است.</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span microsoft="" sans="" serif="" style="font-family:"><b><span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">روشها:</span></span></b> <span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">در این مطالعۀ تجربی، 24 سر رت نر 2 ماهه از نژاد اسپراگداولی با میانگین وزن 20</span></span><span cambria="" lang="FA" style="font-family:"><span style="color:black">±</span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">180 گرم انتخاب شدند. دیابت نوع دو از طریق تزریق محلولهای نیکوتینآمید و استرپتوزوتوسین القاء شد. رتها به روش تصادفی به 4 گروه، 1) گروه کنترل، 2) گروه مکمل، 3) گروه تمرین و 4) گروه تمرین+مکمل تقسیم شدند؛ تمرین مقاومتی شامل 8 هفته و هفتهای 3 جلسه بالارفتن از یک نردبان بود. مکمل منیزیم بهصورت یک بار در روز به رتها خورانده شد. برای تجزیهوتحلیل دادهها از آزمونهای آنوای-یکطرفه و تعقیبی توکی در نرمافزارهای </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">SPSS</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black"> نسخۀ 29 استفاده‏ شد.</span></span><span b="" lang="AR-SA" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="tab-stops:dotted 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span microsoft="" sans="" serif="" style="font-family:"><b><span b="" lang="AR-SA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">یافتهها:</span></span></b> <span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">هشت هفته مصرف مکمل منیزیم و انجام تمرین مقاومتی منجر به تغییر معنیداری در محتوای پروتئینهای </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">CREB2</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black"> و </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">CHOP</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black"> بین گروهها در بطن چپ قلب شد (001/0</span></span> <span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">=</span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">). کاهش معنیداری در گروههای تمرین مقاومتی+مکمل منیزیم و مکمل منیزیم نسبت به گروه کنترل مشاهده شد (05/0</span></span> <span cambria="" lang="FA" math="" style="font-family:"><span style="color:black">≥</span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">)؛ اما گروه تمرین مقاومتی نسبت به گروه کنترل افزایش یافته بود (05/0</span></span> <span cambria="" lang="FA" math="" style="font-family:"><span style="color:black">≥</span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:90%"><span style="tab-stops:dotted 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span microsoft="" sans="" serif="" style="font-family:"><b><span b="" lang="AR-SA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt">نتیجهگیری:</span></span></b> <span b="" lang="FA" lotus="" style="font-family:"><span style="color:black">افزایش و کاهش پروتئینهای </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">CREB2</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black"> و </span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:black">CHOP</span></span></span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="color:black"> در بطن چپ قلب میتواند مانند یک سازِکار دو جانبه، منجر به بهبود و سازگاری فیزیولوژیکی شود.</span></span><span b="" lang="FA" lotus="" style="font-family:"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:110%"><span microsoft="" sans="" serif="" style="font-family:"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Background: </span></span></span></b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Diabetes can disrupt the balance of cell death through different cell pathways, and exercise or consumption supplements can be effective in maintaining the balance of cell death types; Therefore, the purpose of this research is the effect of magnesium supplementation and exercise training on the content of CREB2 and C/EBP homologous protein (CHOP) in the left ventricle of the heart of type 2 diabetic rats.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span microsoft="" sans="" serif="" style="font-family:"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">In this experimental study, 24 2-month-old male Sprague-Dawley rats with an average weight of 280</span></span></span><span style="font-size:10.0pt"><span style="line-height:110%">±</span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">20 grams were selected. Type 2 diabetes was induced by injecting nicotinamide and streptozotocin solutions. The rats were randomly divided into four groups, 1) control, 2) supplement, 3) training and 4) training+supplement; Resistance training consisted of 8 weeks and 3 weekly sessions of climbing a ladder. Magnesium supplement was given to rats once a day. To analyze the data, one-way ANOVA and Tukey's post hoc tests were used in SPSS version 29.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span microsoft="" sans="" serif="" style="font-family:"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Results: </span></span></span></b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Eight weeks of magnesium supplementation and resistance training led to a significant change in the content of CREB2 and CHOP proteins between groups in the left ventricle of the heart (P= 0.001). A significant decrease was observed in the groups of resistance training + magnesium supplement and magnesium supplement compared to the control group (P</span></span></span><span style="font-size:10.0pt"><span style="line-height:110%">≥</span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times=""> 0.05); But the resistance training group had increased compared to the control group (P</span></span></span><span style="font-size:10.0pt"><span style="line-height:110%">≥</span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times=""> 0.05).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span microsoft="" sans="" serif="" style="font-family:"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></span></b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">The increase and decrease of CREB2 and CHOP proteins in the left ventricle of the heart can lead to improvement and physiological adaptation, like a bilateral mechanism.</span></span></span><span lang="AR-SA" dir="RTL" style="line-height:110%"></span></span></span></span>
مکمل منیزیم, تمرین ورزشی, دیابت نوع دو, بطن چپ قلب
Magnesium Supplementation, Exercise Training, Type 2 Diabetes, Left Heart Ventricle
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74
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1167-12&slc_lang=fa&sid=1
Fatemeh
Solhdoust
فاطمه
صلحدوست