fa تعدیل فشار اکسایشی از طریق مسیر انتقال پیام TNFα/SIRT1/NRF1 و عملکرد کلیه با تمرین هوازی با دو شیب متفاوت در موش‌های مبتلا به بیماری مزمن کلیوی (CKD) تخصصي Special پژوهشي Research <span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="fa" style="font-size:11.0pt"><span style="font-weight:bold"><span b="" lotus="" style="font-family:">مقدمه: </span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">هدف از پژوهش ، مقایسۀ تأثیر دونوع تمرین هوازی با و بدون شیب ، بر تعدیل فشار اکسایشی و عملکرد کلیۀ موش مبتلا به بیماری </span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">CKD</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> بوده است.</span></span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="fa" style="font-size:11.0pt"><span style="font-weight:bold"><span b="" lotus="" style="font-family:">روش‌ها: </span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">تعداد ۲۴ سر موش مادۀ نژاد </span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">C57bl6</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> دو ماهه با وزن۲۶/۱</span></span><span lang="ar-SA" style="font-size:11.0pt"><span style="font-family:Cambria">&plusmn;</span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">۲۵ گرم تصادفی به چهارگروه: کنترل سالم،</span></span> <span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">موش‌های مبتلا به </span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">CKD</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">، </span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">CKD</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> و تمرین هوازی بدون شیب و </span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">CKD</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> و تمرین هوازی با شیب تقسیم شدند. به‌منظور القای </span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">CKD</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> پودرآدنین ۲/۰ درصد میلی‌گرم بر کیلوگرم به‌مدت ۴ هفته دریافت کردند. گروه‌ تمرین هشت هفته تمرین تردمیل(۵ جلسه درهفته) یک گروه با شیب ۲۰ درجه و گروه دیگر با</span></span> <span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">شیب صفردرجه</span></span> <span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">اجرا کردند. پس</span></span> <span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">از آخرین جلسۀ تمرین، خون‌گیری به‌منظور اندازه‌گیری نیتروژن اورۀ خون، اوره و غلظت گلوتاتیون به روش الایزا انجام شد. بیان ژن‌های </span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">SIRT1, NRF1, TNF-&alpha;</span></span></span> <span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">با تکنیک </span></span><span lang="en-GB" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">qPCR-Real Time</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> اندازه‌گیری شد. برای تجزیه‌وتحلیل آمار از آزمون آنوا یک‌طرفه و آزمون تعقیبی توکی و سطح معنی‌داری </span></span><span lang="en-US" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"><span style="unicode-bidi:embed">۰۵/</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">0 </span></span><span lang="ar-SA" style="font-size:11.0pt"><span cambria="" math="" style="font-family:">&ge;</span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">P</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> استفاده شد.</span></span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="ar-SA" style="font-size:11.0pt"><span style="font-weight:bold"><span b="" lotus="" style="font-family:">یافته‌ها:</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> نتایج نشان داد القای </span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">CKD</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> موجب افزایش معنی‌دار</span></span> <span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">در مقادیر نیتروژن اورۀ خون و اوره و کاهش معنی‌دار غلظت گلوتاتیون نسبت به گروه کنترل سالم شد (</span></span><span lang="en-US" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"><span style="unicode-bidi:embed">۰۵/</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">0 </span></span><span lang="ar-SA" style="font-size:11.0pt"><span style="font-family:Cambria">></span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">P</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">). از سویی در گروه تمرین با شیب غلظت گلوتاتیون افزایش معنی‌دار و مقادیر نیتروژن اورۀ خون و</span></span> <span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">اوره کاهش معنی‌دار وجود دارد (</span></span><span lang="en-US" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"><span style="unicode-bidi:embed">۰۵/</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">0 </span></span><span lang="ar-SA" style="font-size:11.0pt"><span style="font-family:Cambria">></span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">P</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">). میزان بیان </span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">SIRT1</span></span></span> <span lang="ar-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">و</span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">NRF1</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> در گروه تمرین باشیب افزایش معنی‌دار و میزان بیان</span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">TNF-&alpha; </span></span></span>&nbsp;<span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">کاهش معنی‌دار داشت (</span></span> <span lang="en-US" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"><span style="unicode-bidi:embed">۰۵/</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">0 </span></span><span lang="ar-SA" style="font-size:11.0pt"><span style="font-family:Cambria">></span></span><span lang="en-US" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">P</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">)</span></span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="ar-SA" style="font-size:11.0pt"><span style="font-weight:bold"><span b="" lotus="" style="font-family:">نتیجه‌گیری:</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> تمرینات با شیب سبب تعدیل فشار اکسایشی از طریق مسیرانتقال پیام </span></span><span lang="en-GB" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="unicode-bidi:embed">TNF&alpha;/SIRT1/NRF1</span></span></span><span lang="ar-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> و بهبود دهندۀ غلظت گلوتاتیون و مقادیر نیتروژن اورۀ خون و اوره در موش مبتلا به بیماری مزمن کلیوی می‌شود و اثر تمرین با شیب نسبت به بدون شیب بهتر است.</span></span></span></span><br> &nbsp; <span style="font-size:10.0pt"><span style="font-weight:bold"><span new="" roman="" style="font-family:" times="">Background:</span></span><span new="" roman="" style="font-family:" times=""> The purpose of the research was to compare the effect of two types of aerobic exercise</span><span microsoft="" sans="" serif="" style="font-family:">&mdash;</span><span new="" roman="" style="font-family:" times="">with and without incline</span><span microsoft="" sans="" serif="" style="font-family:">&mdash;</span><span new="" roman="" style="font-family:" times="">on the modulation of oxidative stress and kidney function in chronic kidney disease (CKD) mice.</span></span><br> <span style="font-size:10.0pt"><span style="font-weight:bold"><span new="" roman="" style="font-family:" times="">Methods:</span></span><span new="" roman="" style="font-family:" times=""> A total of 24 two-month-old female C57b16 mice weighing 25</span><span microsoft="" sans="" serif="" style="font-family:">&plusmn;</span><span new="" roman="" style="font-family:" times="">26.1 grams, were randomly divided into four groups: healthy control, CKD-affected mice, CKD mice undergoing aerobic exercise without incline, and CKD mice undergoing aerobic exercise with incline. To induce CKD, they received adenine powder at a concentration of 0.2% mg/kg for four weeks. The training groups performed eight weeks of treadmill training (five sessions per week), whit one group exercising on a 20-degree incline and the other on a zero-degree incline. After the last training session, blood samples were collected to measure blood urea nitrogen, urea and glutathione concentration using the ELISA method. The expression levels of the SIRT1, NRF1 and TNF-</span><span microsoft="" sans="" serif="" style="font-family:">&alpha;</span><span new="" roman="" style="font-family:" times=""> genes were measured using qPCR (Real-Time PCR) technique. For statistical analysis, a one-way ANOVA test and Tukey&#39;s post hoc test were employed, with a significance level set at P</span><span microsoft="" sans="" serif="" style="font-family:">&le;</span><span new="" roman="" style="font-family:" times=""> 0.05.</span></span><br> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold">Results:</span> The results showed that the induction of CKD caused a significant increase in blood urea nitrogen and a significant decrease in glutathione concentration compared to the healthy control group (P< 0.05). Conversely, in the exercise groups, there was a significant increase in glutathione concentration and a significant decrease in blood urea nitrogen (P< 0.05). The expression level of SIRT1 and NRF1 in the slope training group increased significantly, while the expression level of TNF-&alpha; decreased significantly (P< 0.05).</span></span><br> <span style="font-size:10.0pt"><span style="font-weight:bold"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span><span new="" roman="" style="font-family:" times=""> Exercises performed on an incline moderates oxidative stress through the TNF</span><span microsoft="" sans="" serif="" style="font-family:">&alpha;</span><span new="" roman="" style="font-family:" times="">/SIRT1/NRF1 signaling pathway, improving glutathione concentration and blood urea nitrogen levels in mice with chronic kidney disease. Furthermore, the effects of incline exercise were found to be superior to those of exercise without incline.</span></span><br> &nbsp; بیماری مزمن کلیوی (CKD), فشار اکسایشی, تمرین هوازی بدون شیب, تمرین هوازی با شیب, مسیرانتقال پیام TNFα/SIRT1/NRF1 Chronic kidney disease (CKD), Oxidative stress, Non-inclined aerobic exercise, Aerobic exercise with incline, TNFα/SIRT1/NRF1 signaling pathway 118 128 http://ijdld.tums.ac.ir/browse.php?a_code=A-10-704-13&slc_lang=fa&sid=1 Zahra Goli زهرا گلی 0009-0006-4557-9177 No Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran گروه فیزیولوژی ورزشی، دانشکدۀ علوم ورزشی، واحد اصفهان (خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران Farzaneh Taghian فرزانه تقیان ft.taghian@gmail.com 0000-0001-9531-2952 Yes Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran گروه فیزیولوژی ورزشی، دانشکدۀ علوم ورزشی، واحد اصفهان (خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران Khosro Jalali خسرو جلالی khoosro.jalali@gmail.com 0000-0003-0210-5984 No Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran گروه فیزیولوژی ورزشی، دانشکدۀ علوم ورزشی، واحد اصفهان (خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران