Volume 20, Issue 2 (1-2021)
ijdld 2021, 20(2): 105-116 |
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Abdi H, Alijani E, Mohsen zadeh M. THE EFFECT OF INTENSE PERIODIC EXERCISE AND CONSUMPTION OF BLACK GRAPE SEED EXTRACT ON BAX AND BCL-2 GENE EXPRESSION IN PANCREATIC TISSUE OF MALE RATS WITH TYPE 2 DIABETES. ijdld 2021; 20 (2) :105-116
URL: http://ijdld.tums.ac.ir/article-1-5966-en.html
URL: http://ijdld.tums.ac.ir/article-1-5966-en.html
1- Department of Physical Education and Sports Sciences, Karaj Branch, Islamic Azad University, Tehran, Iran , homena.abdi@gmail.com
2- Department of Physical Education and Sports Sciences, Islamic Azad University, Karaj Branch, Alborz, Iran
2- Department of Physical Education and Sports Sciences, Islamic Azad University, Karaj Branch, Alborz, Iran
Abstract: (1963 Views)
Background: Although some studies have studied the mechanism of action of beta cells in animal models and more or less in human populations, but so far the role of exercise therapy or exercise exercise HIIT with black grape supplementation on the expression of genes involved in pancreatic beta cells This study investigates the effect of black grape supplementation combined with intense intermittent exercise on Bcl-2 and Bax genes in pancreatic tissue of rats with type 2 diabetes.
Methods: The study was conducted in the form of an experimental design. The subjects of this project were 40 8-month-old male rats with an average weight of 250 grams. After familiarizing the subjects with exercise and induction of diabetes by STZ, the subjects were randomly divided into 5 groups including exercise, supplement, exercise and supplement, diabetic control and basic control. After 8 weeks of training, which was 5 sessions per week for 8 weeks, the activity was on a treadmill with an intensity of 90% Vo2max and supplementation with black grape seed extract. Bcl-2 and Bax genes were measured after RNA extraction from pancreas and cDNA synthesis. Bcl-2 and Bax genes were measured by Time-Real PCR. Data were analyzed using independent t-test and two-factor analysis of variance at the significance level of 0.05 by SPSS software version 24.
Results: The results showed that the main effect of exercise had no significant effect on Bcl-2 gene expression. The main effect of the extract on the expression of this gene was significant. Also, the interaction between exercise and Bcl-2 supplementation was not significant. Regarding Bax gene expression, it was shown that the main effect of exercise on the gene was significant. The main effect of extract and interaction of exercise and supplementation on Bax gene expression was not significant. No significant changes were observed in the effect of exercise and extract on the expression of Bcl-2 and Bax genes.
Conclusion: It seems that regulating the expression of Bcl-2 and Bax genes through exercise and consumption of black grape seed extract is likely to improve and maintain pancreatic beta cell function in diabetic rats.
Methods: The study was conducted in the form of an experimental design. The subjects of this project were 40 8-month-old male rats with an average weight of 250 grams. After familiarizing the subjects with exercise and induction of diabetes by STZ, the subjects were randomly divided into 5 groups including exercise, supplement, exercise and supplement, diabetic control and basic control. After 8 weeks of training, which was 5 sessions per week for 8 weeks, the activity was on a treadmill with an intensity of 90% Vo2max and supplementation with black grape seed extract. Bcl-2 and Bax genes were measured after RNA extraction from pancreas and cDNA synthesis. Bcl-2 and Bax genes were measured by Time-Real PCR. Data were analyzed using independent t-test and two-factor analysis of variance at the significance level of 0.05 by SPSS software version 24.
Results: The results showed that the main effect of exercise had no significant effect on Bcl-2 gene expression. The main effect of the extract on the expression of this gene was significant. Also, the interaction between exercise and Bcl-2 supplementation was not significant. Regarding Bax gene expression, it was shown that the main effect of exercise on the gene was significant. The main effect of extract and interaction of exercise and supplementation on Bax gene expression was not significant. No significant changes were observed in the effect of exercise and extract on the expression of Bcl-2 and Bax genes.
Conclusion: It seems that regulating the expression of Bcl-2 and Bax genes through exercise and consumption of black grape seed extract is likely to improve and maintain pancreatic beta cell function in diabetic rats.
Keywords: Diabetes, Intense Intermittent Exercise (HIIT), Black Grape Seed Extract, Bcl-2 Protein, Bax Protein, Pancreas
Type of Study: Research |
Subject:
Special
Received: 2020/07/15 | Accepted: 2021/03/1 | Published: 2020/12/30
Received: 2020/07/15 | Accepted: 2021/03/1 | Published: 2020/12/30
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