Volume 1, Issue 1 (18 2001)
ijdld 2001, 1(1): 23-30 |
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Farzami B, Ahmadvand D, Vardasbi S, Majen J, Khaghani S. INDUCTION OF INSULIN BY URTICA DIOICA LEAF EXTRACT IN PERIFUSED ISLETS OF LANGERHANS AND ITS EFFECT IN NORMAL AND DIABETIC RATS. ijdld 2001; 1 (1) :23-30
URL: http://ijdld.tums.ac.ir/article-1-492-en.html
URL: http://ijdld.tums.ac.ir/article-1-492-en.html
Abstract: (21366 Views)
Background: Urtica dioica, or the stinging nettle, is recommended by ancient medical texts for the treatment of high blood sugar.
Methods: We set up a perifusion system, in which an exact number of islets of Langerhans were exposed to an active component of the leaf extract of Urtica dioica, obtained by TLC. The active component was then injected into the peritoneum of both normal and diabetic rats to evaluate response in vivo.
Results: There was a marked increase in insulin secretion in vitro, as determined by ELISA. In vivo, there was an increase in blood insulin content following intraperitoneal injection. The increase in serum insulin observed at 60 minutes was associated with a decrease in blood glucose, checked several times during the observation period. Maximum insulin release over 120 minutes was equal to five times the baseline value. The decrease in blood sugar correlated with both the timing and magnitude of insulin release.
Conclusion: Notwithstanding the magnitude of the changes observed, the results obtained in normal and diabetic rats were similar.
Methods: We set up a perifusion system, in which an exact number of islets of Langerhans were exposed to an active component of the leaf extract of Urtica dioica, obtained by TLC. The active component was then injected into the peritoneum of both normal and diabetic rats to evaluate response in vivo.
Results: There was a marked increase in insulin secretion in vitro, as determined by ELISA. In vivo, there was an increase in blood insulin content following intraperitoneal injection. The increase in serum insulin observed at 60 minutes was associated with a decrease in blood glucose, checked several times during the observation period. Maximum insulin release over 120 minutes was equal to five times the baseline value. The decrease in blood sugar correlated with both the timing and magnitude of insulin release.
Conclusion: Notwithstanding the magnitude of the changes observed, the results obtained in normal and diabetic rats were similar.
Keywords: Urtica dioica, perifusion, insulin secretion, hypoglycemic activity, islets of Langerhans, streptozotocin, diabetes mellitus
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