Volume 19, Issue 4 (4-2020)
ijdld 2020, 19(4): 195-204 |
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Jahani M, Matinhomaie H, Farzanegi P. CHANGES OF PERK AND CHOP PROTEINS IN ENDOPLASMIC RETICULUM OF CARDIAC MYOCYTES AND TNF IN DIABETIC WISTAR RATS FOLLOWING CONTINUOUS AND INTERVAL EXERCISE. ijdld 2020; 19 (4) :195-204
URL: http://ijdld.tums.ac.ir/article-1-5949-en.html
URL: http://ijdld.tums.ac.ir/article-1-5949-en.html
1- Department of physiology exercise, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of physiology exercise, Central Tehran Branch, Islamic Azad University, Tehran, Iran ,hasanmatinhomaee@gmail.com
2- Department of physiology exercise, Central Tehran Branch, Islamic Azad University, Tehran, Iran ,
Abstract: (1761 Views)
Background: Physical activity plays a major role in the prevention of cardiovascular disease and diabetes, but the effect of intense activity on endoplasmic reticulum proteins and apoptosis and necroptosis in diabetic conditions is unclear. The aim of the present study was to investigate the changes of PERK and CHOP proteins in endoplasmic reticulum of cardiac myocytes of diabetic Wistar rats following continuous and interval exercise.
Methods: For this purpose, 32 male white wistar were purchased and were randomly divided into 4 groups of hemogenus 8 rats in each group: Healthy control (C), Diabetic control (D), Diabetic with moderate-intensity continuous training intensity at the 55min on 26 m/min speed (D+MICT) and Diabetic with high-intensity interval training intensity at the 85-90% of maximum speed (D+HIIT); 5 days/week for 8 weeks. For evaluate changes in the expression of the proteins associated with apoptosis and necroptotic death in the diabetic heart muscle myocardium, based on Western blot analysis will be used. Also, the one-way analysis of variance (ANOVA) is used to determine differences between the study groups.
Results: The results showed that induction of type 2 diabetes increased apoptotic and necroptosis cell death (P≥0.05). Therefore, both continuous and intermittent aerobic exercise modulate apoptotic cell death. And both intermittent and continuous exercise had a significant effect on cell necroptosis death.
Conclusion: It seems that different levels of aerobic exercise have different effects on cardiac myocytes cell death in diabetic rats. But more research is needed to confirm the death of diabetic necroptics.
Methods: For this purpose, 32 male white wistar were purchased and were randomly divided into 4 groups of hemogenus 8 rats in each group: Healthy control (C), Diabetic control (D), Diabetic with moderate-intensity continuous training intensity at the 55min on 26 m/min speed (D+MICT) and Diabetic with high-intensity interval training intensity at the 85-90% of maximum speed (D+HIIT); 5 days/week for 8 weeks. For evaluate changes in the expression of the proteins associated with apoptosis and necroptotic death in the diabetic heart muscle myocardium, based on Western blot analysis will be used. Also, the one-way analysis of variance (ANOVA) is used to determine differences between the study groups.
Results: The results showed that induction of type 2 diabetes increased apoptotic and necroptosis cell death (P≥0.05). Therefore, both continuous and intermittent aerobic exercise modulate apoptotic cell death. And both intermittent and continuous exercise had a significant effect on cell necroptosis death.
Conclusion: It seems that different levels of aerobic exercise have different effects on cardiac myocytes cell death in diabetic rats. But more research is needed to confirm the death of diabetic necroptics.
Keywords: Pancreas Endoplasmic Reticulum Kinase, CHOP, Apoptosis, Necroptosis, Diabetes, Continuous And Interval Exercise
Type of Study: Research |
Subject:
General
Received: 2020/05/27 | Accepted: 2020/08/19 | Published: 2020/04/29
Received: 2020/05/27 | Accepted: 2020/08/19 | Published: 2020/04/29
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