Sharifi Rayeni M, Daryanoosh F, Salesi M, Kooshki Jahromi M. The Effect of High-Intensity Interval Training (HIIT) on the Content of Proteins Related to the Mitophagy Pathway (LC3 And BNIP3L) in the Muscle Tissue Soleus of Rats with Type 2 Diabetes. ijdld 2024; 24 (2) :124-132
URL:
http://ijdld.tums.ac.ir/article-1-6296-en.html
1- Department of Sport Sciences, School of Education and Psychology, Shiraz university, Shiraz, Iran
2- Department of Sport Sciences, School of Education and Psychology, Shiraz university, Shiraz, Iran , daryanoosh@shirazu.ac.ir
Abstract: (586 Views)
Background: Mitophagy is a type of cell death that regulates the quality of mitochondria and can lead to disorders in diseases such as diabetes. Therefore, the purpose of this study was to investigate the effect of high-intensity interval training (HIIT) on the content of proteins related to the mitophagy pathway (LC3 and BNIP3L) in muscle tissue soleus of rats with type 2 diabetes.
Methods: In this experimental study, 18 three-month-old male Sprague Dawley rats with an average weight of 270±30 g were selected. Rats were infected with type 2 diabetes by intraperitoneal injection of a streptozotocin and nicotinamide solution. Rats were randomly divided into two groups: diabetic and diabetic. A healthy control group was also included. The training group performed HIIT for eight weeks at an intensity of 85-95% of the maximum speed. Data analysis was performed using a one-way ANOVA test in GraphPad Prism version 9.5 software. A significance level of P≤ 0.05 was considered statistically significant.
Results: The levels of LC3 and BNIP3L proteins significantly increase after eight weeks of HIIT compared to both the diabetic and healthy control groups (P= 0.0001).
Conclusion: It can be concluded that HIIT by increasing the factors related to mitophagy can cause the cleaning of dysfunctional mitochondria in the muscle of diabetic subjects; However, excessive mitophagy can also cause functional defects in regulating the quality of mitochondria.
Type of Study:
Research |
Subject:
Special Received: 2023/10/7 | Accepted: 2024/01/10 | Published: 2024/06/30