1- University of Guilan
2- University of Guilan ,Mohebbi_h@yahoo.com
3- University of Mohaghegh Ardabili
2- University of Guilan ,
3- University of Mohaghegh Ardabili
Abstract: (462 Views)
Background: Adipose tissue fibrosis is involved in glucose metabolism disorder and insulin resistance in obesity, but the effect of exercise on the progression of adipose tissue fibrosis is still unknown.
This study aimed to investigate the effect of high intensity interval training (HIIT) simultaneously with high-fat diet on TGF-β1 , MMP-9 and MMP-2 in the subcutaneous adipose tissue of male rats.
Method: 24 male rats were randomly divided into 4 groups: normal diet (ND) , high fat diet (HFD) , Normal diet + high intensity interval training(ND+HIIT), high fat diet+ high intensity interval training(HFD+HIIT).
The HIIT protocol, including 8 bouts of intense activity at 90% of maximum running capacity (MRC) for 2.5 minutes, with active rest periods at 50% of maximum running capacity for 2.5 minutes for 12 weeks(5 sessions per week).
48 hours after the last training session, blood was taken and subcutaneous fat was removed. Western blot method was used evaluate the TGF-β1 and ELISA method was used to measure levels of MMP-9, MMP-2, insulin.
Results: Induction of obesity was associated with a significant increase in TGF-β1,MMP-9 and MMP-2 and insulin resistance (P˂0.0001). In contrast, high-intensity interval with high fat diet compared to the high fat diet group causes a significant decrease in the amount of TGF-β1, MMP-9, MMP-2, and insulin resistance (P˂0.0001).
Conclusion: In conclusion, our data indicate that High-intensity interval training may weaken the progression of adipose tissue fibrosis and have a preventive effect on the increase in glucose metabolism disorders caused by a high-fat diet.
This study aimed to investigate the effect of high intensity interval training (HIIT) simultaneously with high-fat diet on TGF-β1 , MMP-9 and MMP-2 in the subcutaneous adipose tissue of male rats.
Method: 24 male rats were randomly divided into 4 groups: normal diet (ND) , high fat diet (HFD) , Normal diet + high intensity interval training(ND+HIIT), high fat diet+ high intensity interval training(HFD+HIIT).
The HIIT protocol, including 8 bouts of intense activity at 90% of maximum running capacity (MRC) for 2.5 minutes, with active rest periods at 50% of maximum running capacity for 2.5 minutes for 12 weeks(5 sessions per week).
48 hours after the last training session, blood was taken and subcutaneous fat was removed. Western blot method was used evaluate the TGF-β1 and ELISA method was used to measure levels of MMP-9, MMP-2, insulin.
Results: Induction of obesity was associated with a significant increase in TGF-β1,MMP-9 and MMP-2 and insulin resistance (P˂0.0001). In contrast, high-intensity interval with high fat diet compared to the high fat diet group causes a significant decrease in the amount of TGF-β1, MMP-9, MMP-2, and insulin resistance (P˂0.0001).
Conclusion: In conclusion, our data indicate that High-intensity interval training may weaken the progression of adipose tissue fibrosis and have a preventive effect on the increase in glucose metabolism disorders caused by a high-fat diet.
Article number: 11
Keywords: adipose tissue fibrosis, matrix metalloproteinase, high intensity interval training, insulin resistance
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