Volume 24, Issue 6 (2-2025)
ijdld 2025, 24(6): 397-404 |
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Alizadeh pahlavani H. Effect of Moderate-Intensity Interval Training (MIIT) on Total and Phosphorylated Content of Ribosomal Protein S6K1 in Heart Tissue of Type 2 Diabetic Rats. ijdld 2025; 24 (6) :397-404
URL: http://ijdld.tums.ac.ir/article-1-6341-en.html
URL: http://ijdld.tums.ac.ir/article-1-6341-en.html
Department of Physical Education, Farhangian University, Tehran, Iran , ha.alizadeh@cfu.ac.ir
Abstract: (625 Views)
Background: Type 2 diabetes is characterized by insulin resistance and hyperglycemia and can lead to heart disease. Therefore, the aim of the present study is to investigate the effect of MIIT on the S6K1 pathway in the myocardium, which is related to the control of cell growth and proliferation.
Methods: In this study, 12 two-month-old male Sprague Dawley rats with an average weight of 280±30 grams participated. To induce diabetes, nicotinamide and streptozotocin solutions were injected with a dose of 110 mg/kg and 60 mg/kg, respectively. The blood sugar of rats was determined between 126-260 mg/dL as an indicator of type 2 diabetes. After the induction of diabetes, the rats were randomly divided into diabetic training group (6 heads) and diabetic control group (6 heads). The diabetic training group trained for 4 weeks and 4 sessions every week. 24 hours after the last training session, the left ventricle of heart was isolated and the amount of protein was measured by western blotting method. Variables were analyzed through independent t-tests. The significance level of study was considered P≤0.05.
Results: Data analysis showed that the intracellular content of total (P=0.62), phosphorylated (P=0.85), and total to phosphorylated (P=0.77) S6K1 protein did not show significant changes after 4 weeks of MIIT.
Conclusion: It seems that after 4 weeks of MIIT, S6K1 protein does not change significantly, so it seems that the duration and intensity of training and nutritional conditions to increase S6K1 phosphorylation should be considered in future research
Methods: In this study, 12 two-month-old male Sprague Dawley rats with an average weight of 280±30 grams participated. To induce diabetes, nicotinamide and streptozotocin solutions were injected with a dose of 110 mg/kg and 60 mg/kg, respectively. The blood sugar of rats was determined between 126-260 mg/dL as an indicator of type 2 diabetes. After the induction of diabetes, the rats were randomly divided into diabetic training group (6 heads) and diabetic control group (6 heads). The diabetic training group trained for 4 weeks and 4 sessions every week. 24 hours after the last training session, the left ventricle of heart was isolated and the amount of protein was measured by western blotting method. Variables were analyzed through independent t-tests. The significance level of study was considered P≤0.05.
Results: Data analysis showed that the intracellular content of total (P=0.62), phosphorylated (P=0.85), and total to phosphorylated (P=0.77) S6K1 protein did not show significant changes after 4 weeks of MIIT.
Conclusion: It seems that after 4 weeks of MIIT, S6K1 protein does not change significantly, so it seems that the duration and intensity of training and nutritional conditions to increase S6K1 phosphorylation should be considered in future research
Keywords: Moderate-intensity interval training (MIIT), Ribosomal protein S6K1, Heart, Type 2 diabetes
Type of Study: Research |
Subject:
Special
Received: 2024/04/13 | Accepted: 2023/05/31 | Published: 2025/02/28
Received: 2024/04/13 | Accepted: 2023/05/31 | Published: 2025/02/28
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